Meeting Minutes for July 7, 2006
From 2006.igem.org
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Minutes for Weekly meeting (7/7) | Minutes for Weekly meeting (7/7) | ||
- | Indiv. Member Responsibilities: still underway | + | '''Indiv. Member Responsibilities: still underway''' |
- | + | '''Freeze tag:''' | |
- | + | ||
- | Freeze tag: | + | |
Problems: | Problems: | ||
-MotB flagellar machinery is intact, not structurally changing bacteria, just the motor (good) | -MotB flagellar machinery is intact, not structurally changing bacteria, just the motor (good) | ||
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-LVA: is it made individually? Cell recognizes it as mRNA | -LVA: is it made individually? Cell recognizes it as mRNA | ||
- | Sender cell: all parts grown up, minipreps on all parts. Colonies didn’t grow | + | '''Sender cell:''' all parts grown up, minipreps on all parts. Colonies didn’t grow |
run another gel, digest it, get it sequenced and ligate the 3 parts together | run another gel, digest it, get it sequenced and ligate the 3 parts together | ||
-will sequence after first digestion (Brendan) | -will sequence after first digestion (Brendan) | ||
-designing the primers: Annie and Prof. Wessel | -designing the primers: Annie and Prof. Wessel | ||
Receiver cell: need to run digest of the two parts (Victoria and Megan) | Receiver cell: need to run digest of the two parts (Victoria and Megan) | ||
- | “Freeze Machine”: need to incubate cells, do minipreps | + | '''“Freeze Machine”:''' need to incubate cells, do minipreps |
- | Unfreeze: do miniprep tonight (Jason and Azeem) | + | '''Unfreeze:''' do miniprep tonight (Jason and Azeem) |
- | Modeling Mechanism | + | '''Modeling Mechanism''' |
-put a gene then a protein with a line connecting it, RBS and primer is an effector of that pathway | -put a gene then a protein with a line connecting it, RBS and primer is an effector of that pathway | ||
- | + | Problems: can’t inhibit the rate the promoter outputs | |
-talks about having a metabox | -talks about having a metabox | ||
-website: bio tapestry | -website: bio tapestry | ||
- | What are hopes of model? | + | '''What are hopes of model?''' |
-10 internal signaling pathways, worth running time courses seeing how stable system is | -10 internal signaling pathways, worth running time courses seeing how stable system is | ||
-does system break down if we vary concentrations? | -does system break down if we vary concentrations? | ||
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-look into buying AHL | -look into buying AHL | ||
- | Magnetotacticbacteria | + | '''Magnetotacticbacteria''' |
-still alive | -still alive | ||
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-look at article with Mag A, | -look at article with Mag A, | ||
- | Tasks that members have taken on: | + | '''Tasks that members have taken on:''' |
-Megan will look at options to synthesize avadin from scratch. Costs ? Practicality ? | -Megan will look at options to synthesize avadin from scratch. Costs ? Practicality ? | ||
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you can update the particular parts for “the Freeze Tag” | you can update the particular parts for “the Freeze Tag” | ||
- | -General overview of Project (Azeem) | + | -'''General overview of Project (Azeem)''' |
- | Outreach Coordinator: Megan | + | '''Outreach Coordinator: Megan''' |
- | Industry Liaison: Victoria | + | '''Industry Liaison: Victoria''' |
- | Future Planning: Jason, Brendan, John Cumbers | + | '''Future Planning: Jason, Brendan, John Cumbers''' |
Revision as of 19:01, 7 July 2006
Minutes for Weekly meeting (7/7)
Indiv. Member Responsibilities: still underway
Freeze tag: Problems: -MotB flagellar machinery is intact, not structurally changing bacteria, just the motor (good) - it bridges into to the cell, shouldn’t cause a quantum increase -LVA: is it made individually? Cell recognizes it as mRNA
Sender cell: all parts grown up, minipreps on all parts. Colonies didn’t grow run another gel, digest it, get it sequenced and ligate the 3 parts together -will sequence after first digestion (Brendan) -designing the primers: Annie and Prof. Wessel Receiver cell: need to run digest of the two parts (Victoria and Megan) “Freeze Machine”: need to incubate cells, do minipreps Unfreeze: do miniprep tonight (Jason and Azeem)
Modeling Mechanism -put a gene then a protein with a line connecting it, RBS and primer is an effector of that pathway
Problems: can’t inhibit the rate the promoter outputs -talks about having a metabox -website: bio tapestry
What are hopes of model? -10 internal signaling pathways, worth running time courses seeing how stable system is -does system break down if we vary concentrations? -is there a way to do positive control, repressalator -To check that logical sensible -want to see if signaling cell will actually stop the receiver cell, if not, can we figure out the strength of the RBS necessary to get the job done -focus of small steps: once we get the sensor and sender of AHL working we can then test it out to define rate laws (do so by putting GFP at end of sequences-to test the POPs)
How do we test AHL concentration (it is a lactose analog-a simple carbohydrate)?
Right now we are using TetR promoter, will that be strong enough? Not that well characterisized. -Brendan suggesting making a biobrick with T4 promoter (wouldn’t be that hard) -Think we need 0.2 nanomoles of AHL to activate the AHL receiver
-look into buying AHL
Magnetotacticbacteria
-still alive -looked at most concentrated vile, 3 cells in each visual field 200microliters, 50 microliters of food -divided to some extent -mixed up another vile to grow in anerobic bacterial incubator -Hyato requested 4 types of plasmid, that Luciferase expresses much better, email has been sent out -Plamids modified in D5Halpha cells -can electroborate into bacterial cells and the other to conjugate them - before we can do anything we need to get a stable stock of the bacteria -will try plating the bacteria now that we have access to anerobic chamber
-can a biobick could be created for the plasmids?
-we should get a hold of avadin part, then optimize codon usage, find restriction sites -look at article with Mag A,
Tasks that members have taken on:
-Megan will look at options to synthesize avadin from scratch. Costs ? Practicality ? -end goal to make a standard shuttle vector that can be used
-take origin of replication from plasmids that we are looking for and insert them into the shuttle vector as a contribution to the biobricks registry. Important to make it compatible with most bacteria used in the project (Jason)
-wiki has been updated by Annie and Jamie you can update the particular parts for “the Freeze Tag”
-General overview of Project (Azeem)
Outreach Coordinator: Megan
Industry Liaison: Victoria
Future Planning: Jason, Brendan, John Cumbers