PSB1A7
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| - | To prevent uncontrolled expression of parts within our device, we built insulated cloning vector pSB1A7 (<partinfo>BBa_J31009</partinfo>). pSB1A7 was built using the pre-existing <partinfo>pSB1A3</partinfo> vector. The terminators in pSB1A3 are designed to prevent read-through from inside the device out into the vectr (purple arrows) but the vector is not designed to stop read-through into the vector (red arrows). We added double terminators (<partinfo>BBa_B0015</partinfo>) in opposite orientations at each end of the BioBrick cloning site region to shield the insert from transcription read-through. When the pancakes are cloned into pSB1A7, tetracycline resistance is dependent on the proper orientation of the two DNA pancakes. | + | To prevent uncontrolled expression of parts within our device, we built insulated cloning vector '''pSB1A7''' (<partinfo>BBa_J31009</partinfo>). pSB1A7 was built using the pre-existing <partinfo>pSB1A3</partinfo> vector. The terminators in pSB1A3 are designed to prevent read-through from inside the device out into the vectr (purple arrows) but the vector is not designed to stop read-through into the vector (red arrows). We added double terminators (<partinfo>BBa_B0015</partinfo>) in opposite orientations at each end of the BioBrick cloning site region to shield the insert from transcription read-through. When the pancakes are cloned into pSB1A7, tetracycline resistance is dependent on the proper orientation of the two DNA pancakes. |
See [http://partsregistry.org/Part:BBa_J31009:Design the part design page] for details on how pSB1A7 was built. | See [http://partsregistry.org/Part:BBa_J31009:Design the part design page] for details on how pSB1A7 was built. | ||
Revision as of 22:27, 30 October 2006
Our new pSB1A7 cloning vector blocks read-through transcription
To prevent uncontrolled expression of parts within our device, we built insulated cloning vector pSB1A7 (). pSB1A7 was built using the pre-existing vector. The terminators in pSB1A3 are designed to prevent read-through from inside the device out into the vectr (purple arrows) but the vector is not designed to stop read-through into the vector (red arrows). We added double terminators () in opposite orientations at each end of the BioBrick cloning site region to shield the insert from transcription read-through. When the pancakes are cloned into pSB1A7, tetracycline resistance is dependent on the proper orientation of the two DNA pancakes.
See [http://partsregistry.org/Part:BBa_J31009:Design the part design page] for details on how pSB1A7 was built.
