PSB1A7
From 2006.igem.org
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| - | To prevent uncontrolled expression of parts within our device, we built insulated cloning vector pSB1A7 (<partinfo>BBa_J31009</partinfo>). pSB1A7 was built using the pre-existing <partinfo>pSB1A3</partinfo> vector. The terminators in pSB1A3 are designed to prevent read-through from inside the device out into the vectr (purple arrows) but the vector is not designed to stop read-through into the vector (red arrows). We added double terminators (<partinfo>BBa_B0015</partinfo>) in opposite orientations at each end of the BioBrick cloning site region to shield the insert from transcription read-through. When the pancakes are cloned into pSB1A7, tetracycline resistance is dependent on the proper orientation of the two DNA pancakes. | + | To prevent uncontrolled expression of parts within our device, we built insulated cloning vector '''pSB1A7''' (<partinfo>BBa_J31009</partinfo>). pSB1A7 was built using the pre-existing <partinfo>pSB1A3</partinfo> vector. The terminators in pSB1A3 are designed to prevent read-through from inside the device out into the vectr (purple arrows) but the vector is not designed to stop read-through into the vector (red arrows). We added double terminators (<partinfo>BBa_B0015</partinfo>) in opposite orientations at each end of the BioBrick cloning site region to shield the insert from transcription read-through. When the pancakes are cloned into pSB1A7, tetracycline resistance is dependent on the proper orientation of the two DNA pancakes. |
See [http://partsregistry.org/Part:BBa_J31009:Design the part design page] for details on how pSB1A7 was built. | See [http://partsregistry.org/Part:BBa_J31009:Design the part design page] for details on how pSB1A7 was built. | ||
Revision as of 22:27, 30 October 2006
Our new pSB1A7 cloning vector blocks read-through transcription
To prevent uncontrolled expression of parts within our device, we built insulated cloning vector pSB1A7 (). pSB1A7 was built using the pre-existing vector. The terminators in pSB1A3 are designed to prevent read-through from inside the device out into the vectr (purple arrows) but the vector is not designed to stop read-through into the vector (red arrows). We added double terminators () in opposite orientations at each end of the BioBrick cloning site region to shield the insert from transcription read-through. When the pancakes are cloned into pSB1A7, tetracycline resistance is dependent on the proper orientation of the two DNA pancakes.
See the part design page for details on how pSB1A7 was built.
