Penn State University 2006

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__NOTOC__
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<font color="#FFFFFF" size="2">The bacterial relay race takes advantage of an ability to control cellular motility using inducible promoters such as those involved in nutrient catabolism or quorum sensing. “Receiver” bacteria move in response to small-molecule signals either added to the system or originating from motile, “sender” strains. The most significant challenges relating to this project stem from difficulties of tightly controlling the target motility gene motB. Low levels of motB expression result in system failure (constitutive motility), and resolving this issue is essential to developing reliable modular systems that are the hallmark of synthetic biology.</font>
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<center><h3 style="color: white">Team members</h3></center>
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*[[user:jbad|<font color="#99cccc">Jon Bad</font>]] 
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*[[user:cbuck|<font color="#99cccc">Chris Buck</font>]]
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*[[user:jgrim|<font color="#99cccc">Joel Q. Grim</font>]] 
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*[[user:RSand|<font color="#99cccc">Ralphy</font>]]
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*[[user:ttasc|<font color="#99cccc">Tony</font>]] 
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*[[user:ljw|<font color="#99cccc">LJ</font>]]
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*[[user:luw|<font color="#99cccc">Luc</font>]]
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<center><h3 style="color: white">Faculty</h3></center>
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*[[user:Pcir|<font color="#99cccc">Pat Cirino</font>]] 
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<font face="Tahoma" color="#FFFFFF">
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*[[user:Whan|<font color="#99cccc">Will Hancock</font>]]
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<font color="#B0C8AE" size="6">>People</font>
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*[[user:TRich|<font color="#99cccc">Tom Richard</font>]] 
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*[[user:Dfarb|<font color="#99cccc">Darryl Farber</font>]]
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*[[user:MTien|<font color="#99cccc">Ming Tien</font>]] 
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*[[user:PW|<font color="#99cccc">Paul Weiss</font>]]|
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<center><h3 style="color: white">Affiliated Grad Students</h3></center>
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<h2 style="color: #C5D1D7">'''Undergraduate Team'''</h2>
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*[[user:Pcir|<font color="#99cccc">Steve Walker</font>]]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Jon Badalamenti]
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*[[user:Whan|<font color="#99cccc">Deepti Tanjore</font>]]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Lucien Weiss]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Chris Buckno]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Joel Q. Grim</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Ralph Sandoval</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Barbara Andersen</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Jen Dunning</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Natalie Shewski</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">John Bigus</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Aleksandr Lewicki</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Elizabeth Morin</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Yan Shipelskiy</font>]
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<h2 style="color: #C5D1D7">'''Affiliated Grad Students'''</h2>
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Steve Walker</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Deepti Tanjore</font>]
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<h2 style="color: #C5D1D7">'''Faculty'''</h2>
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Tom Richard</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Pat Cirino</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Will Hancock</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Darryl Farber</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Ming Tien</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Paul Weiss</font>]
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<center><h3 style="color: white">Project</h3></center>
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<h2 style="color: #C5D1D7">'''Graduated Team Members'''</h2>
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*[http://2006.igem.org/wiki/index.php/Penn_State_2005 Penn State iGEM 2005]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Lane J. Weaver</font>]
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*[[Penn_State_University_2006:PSUprotocols|<font color="#99cccc">Protocols</font>]]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Tony Tascone</font>]
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**[[Penn_State_University_2006:Cloning|<font color="#99cccc">Cloning</font>]]  
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Steve Neuman</font>]
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***[[Penn_State_University_2006:Preparing chemically competent cells|<font color="#99cccc">Preparing chemically competent cells</font>]] 
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***[[Penn_State_University_2006:Restriction|<font color="#99cccc">Restriction</font>]]
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***[[Penn_State_University_2006:Ligation|<font color="#99cccc">Ligation</font>]]
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***[[Penn_State_University_2006:Transformation|<font color="#99cccc">Transformation</font>]]
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**[[Penn_State_University_2006:Plate-Reading|<font color="#99cccc">Plate-reading</font>]]
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**[[Penn_State_University_2006:Microscopy|<font color="#99cccc">Microscopy</font>]]  
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**[[Penn_State_University_2006:Motility-assays|<font color="#99cccc">Motility-assays</font>]]
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**[[Penn_State_University_2006:Microchannel|<font color="#99cccc">Microchannel</font>]]
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**[[Penn_State_University_2006:Strain Construction|<font color="#99cccc">Strain Construction</font>]] 
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*[[Penn_State_University_2006:PSUinventory|<font color="#99cccc">Inventory</font>]]
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**[[Penn_State_University_2006:PSUinventory_Registry parts|<font color="#99cccc">Registry parts</font>]]
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**[[Penn_State_University_2006:PSUinventory_Constructed parts|<font color="#99cccc">Constructed parts</font>]]
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**[[Penn_State_University_2006:PSUinventory_Cell strains|<font color="#99cccc">Cell strains</font>]]
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**[[Penn_State_University_2006:PSUinventory_Enzymes|<font color="#99cccc">Enzymes</font>]]
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**[[Penn_State_University_2006:PSUinventory_Equipment|<font color="#99cccc">Equipment</font>]]   
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<center><h3 style="color: white">Schedule/Progress</h3></center>
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<h2 style="color: #C5D1D7">'''Photos'''</h2>
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*<font color="#99cccc">Progress</font>
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Lab Photos</font>]  
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**[[Penn_State_University_2006:Progress_cloning|<font color="#99cccc">Cloning results</font>]]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Team Member Photos</font>]
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**[[Penn_State_University_2006:Progress_motility|<font color="#99cccc">Motility results</font>]]
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**[[Penn_State_University_2006:Progress_plate-reading|<font color="#99cccc">Plate-reading</font>]]
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**[[Penn_State_University_2006:Progress_microchannels|<font color="#99cccc">Microchannels results</font>]]
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*<font color="#99cccc">Schedule</font>
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**[[Penn_State_University_2006:trivia|<font color="#99cccc">Wed Night trivia results</font>]]
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*[[Penn_State_University_2006:calendar|<font color="#99cccc">Calendar</font>]]
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<center>[[Image:Psulogo.JPG]]</center>
 
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<font face="Tahoma" color="#FFFFFF">
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<font color="#646B86" size="6">>Project</font>
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<center><h3 style="color: white">Background/Links</h3></center>
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<center>[[Image:Psulogo.JPG]]</center>
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<h2 style="color: #D16349">'''Publications'''</h2>
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#000000">Synthetic Sports: A Bacterial Relay Race</font>]
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<h2 style="color: #D16349">'''Protocols'''</h2>
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#000000">Cloning</font>]
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**[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#000000">Preparing chemically competent cells</font>]
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**[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#000000">Growing Parts</font>]
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**[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#000000">Restriction</font>]
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**[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#000000">Ligation</font>]
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**[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#000000">Transformation</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#000000">Plate-reading</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#000000">Motility-assays</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#000000">Microchannel</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#000000">Strain Construction</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#000000">Inventory</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#000000">DNA Gel</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#000000">Growth Media</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#000000">LB Plates</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#000000">Eiken Agar Plates</font>]
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<h2 style="color:#D16349">'''Progress'''</h2>
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#000000">Submitted Registry Parts</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#000000">Strain-Construction Progress</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#000000">Microchannels results</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#000000">Motility results</font>]
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<h2 style="color: #D16349">'''Background'''</h2>
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*[[http://openwetware.org/wiki/IGEM:PennState/2006|<font color="#000000">Development of the Ecoli Relay Race</font>]]
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*[http://en.wikipedia.org/wiki/Relay_race Wikipedia: Relay Race]
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<h2 style="color: #D16349">'''Links'''</h2>
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*[[iGEM|iGEM on OpenWetWare]]
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*[http://partsregistry.org Parts registry]
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*[http://2007.igem.org iGEM wiki]
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*[http://2006.igem.org/wiki/index.php/Penn_State_2005 Penn State iGEM 2005]
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*[http://2006.igem.org/wiki/index.php/Penn_State_University_2006 Penn State iGEM 2006]

Latest revision as of 20:07, 9 March 2007

PSU banner-logo1.jpg Oldmain3.jpg

The bacterial relay race takes advantage of an ability to control cellular motility using inducible promoters such as those involved in nutrient catabolism or quorum sensing. “Receiver” bacteria move in response to small-molecule signals either added to the system or originating from motile, “sender” strains. The most significant challenges relating to this project stem from difficulties of tightly controlling the target motility gene motB. Low levels of motB expression result in system failure (constitutive motility), and resolving this issue is essential to developing reliable modular systems that are the hallmark of synthetic biology.

>People

Undergraduate Team

Affiliated Grad Students

Faculty

Graduated Team Members

Photos

>Project

Publications

Protocols

Progress

Background

Links

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