Phase 1: PCR Amplification of Genes

From 2006.igem.org

A. Isolating genes for the quorum sensing mechanism from genomic B. subtilis DNA

1. ComX - pheromone, 168bp

• Primers: Fwd = CGGAATTCGCTCTAGATGCAAGACCTAATTAACTACTTTTTAAATTATCC

                     Rev = GACTGCAGCTACTAGTTAATCACCCCATTGACGGGTTATTGG

• Notes:

Forward primer contains EcoRI and XbaI cut sites; Reverse primer contains SpeI and PstI cut sites

Amplification of ComX alone was unsuccessful. However, ComQ and ComX are adjacent on the genome so we subsequently amplified them together