Phase 2: Plasmid Construction and Bacterial Transformations
From 2006.igem.org
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'''3. Significance: | '''3. Significance: | ||
::*We now have all the fundamental components for the project ahead | ::*We now have all the fundamental components for the project ahead | ||
| - | ::*Can proceed in constructing the | + | ::*Can proceed in constructing the hybrid quorumtaxis receptor using ComP and Tsr genes |
::*Will need to perform quick-change mutagenesis for ComA to remove internal SpeI site | ::*Will need to perform quick-change mutagenesis for ComA to remove internal SpeI site | ||
Revision as of 19:17, 30 October 2006
1. Isolated ptetRBS and pSB1A3 plasmids from BioBricks
- Attached tet promoter with Ribosome Binding Site to GFP gene, transformed XL-1 E. coli and observed positive expression
2. Ligated ComQX/P/A and Tsr fragments from Phase 1 into pSB1A3 plasmids
- Used SpeI and XbaI restriction sites
- Verified transformation for all fragments and sequenced genes (results positive in all cases).
3. Significance:
- We now have all the fundamental components for the project ahead
- Can proceed in constructing the hybrid quorumtaxis receptor using ComP and Tsr genes
- Will need to perform quick-change mutagenesis for ComA to remove internal SpeI site
