Plasmids and Bacteria

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* Create RE biobrick (completed)
* Create RE biobrick (completed)
-
*Isolating genomic DNA from Salmonella typhimurium
+
*Isolating genomic DNA from Salmonella typhimurium (completed)
*Ordered oligos for recombinational enhancer and primers for the hin gene
*Ordered oligos for recombinational enhancer and primers for the hin gene
 +
 +
These sequences will be transformed into RFP and possibly GRP plasmids

Revision as of 13:12, 7 June 2006

  • pSB1A3
      • amp resistant
      • copy number:100-300
      • size:2157 empty
  • pSB1AC3
      • part-BBAp1000
      • copy number:100-3000
      • amp and CmR resistant
      • contains device that provides CmR
      • size:3844 ( with device) 3005 (without device)
  • pSB1AK3
      • part BBa_|13535
      • copy number:100-300
      • amp and kan resistance
      • contains a device for screening test
      • size: 3457 (with device) 3189 (without device)
  • Transform bacteria We are using Salmonella typhimurium for the Hin protein as it was the referenced species in all of the literature.
  • Create RE biobrick (completed)
  • Isolating genomic DNA from Salmonella typhimurium (completed)
  • Ordered oligos for recombinational enhancer and primers for the hin gene

These sequences will be transformed into RFP and possibly GRP plasmids

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