Presentation

From 2006.igem.org

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	== Presentation Script ==		== Presentation Script ==
-	Script:	+	====Slide 1====
		+	* Introduce the team (i.e. collaborative effort between UofT and UW) and mention the project we're working on.
-	Slide 1:	+	====Slide 2====
-	- Introduce the team (i.e. collaborative effort between UofT and UW) and mention the project we're working on.	+	* Explain what makes this thermometer unique from all other existing models as in other models may limitations to where/how they can be used
		+	** size of the species of interest may be too small
		+	** research experiments where chemicals, radiation may be toxic for a colony but temperature readings are needed
-	Slide 2:	+	* A bio-synthetic thermometer may be a possible solution to overcome these obstacles.
-	- Explain what makes this thermometer unique from all other existing models as in other models may limitations to where/how they can be used	+
-	- size of the species of interest may be too small	+
-	- research experiments where chemicals, radiation may be toxic for a colony but temperature readings are needed	+
-	- A bio-synthetic thermometer may be a possible solution to overcome these obstacles.	+	* Applications:
		+	** use to detect small changes in temperature in very small organisms- we can do this by measuring the intensity of the colour emitted
		+	** experiments or places where a quantitative reading cannot be obtained (i.e a person can not physically be there to take the reading (in anaerobic conditions?)) or where air pressure
		+	** may also use this to monitor temperature of aquariums using zebra fish for example
-	- Applications:	+	====Slide 3====
-	- use to detect small changes in temperature in very small organisms- we can do this by measuring the intensity of the colour emitted	+	* Explain diagrams
-	- experiments or places where a quantitative reading cannot be obtained (i.e a person can not physically be there to take the reading (in anaerobic conditions?)) or where air pressure	+
-	- may also use this to monitor temperature of aquariums using zebra fish for example	+
-	Slide 3:	+	====Slide 4====
-	- Explain diagrams	+	* Explain our approach: how we decided to ligate parts separately and build up to the final product
		+	* Constructed many new parts
		+	* Replaced our cI repressor system with tetracycline repressor (tetR), an analogous system to cI but less leaky and can control externally
-	Slide 4:	+	====Slide 5====
-	- Explain our approach: how we decided to ligate parts separately and build up to the final product	+	* Testing Phase
-	- Constructed many new parts	+	** Intro: - Testing was split into 3 modules.
-	- Replaced our cI repressor system with tetracycline repressor (tetR), an analogous system to cI but less leaky and can control externally	+	*** Each module acts as a checkpoint for a particular milestone in the construction process.
		+	*** After each module we can ensure a vital component of the construct is functional.
		+	** State the goal/purpose of each module and how it was executed.
-	Slide 5:	+	====Slide 6====
-	- Testing Phase	+	* Test Results
-	- Intro: - Testing was split into 3 modules.	+	** Tested the intermediary pBad/araC + LacI ts coding sequence (R0011) + reporter gene thoroughly
-	- Each module acts as a checkpoint for a particular milestone in the construction process.	+	** We ensured that module 1 tests passed before continuing construction.
-	- After each module we can ensure a vital component of the construct is functional.	+
-	- State the goal/purpose of each module and how it was executed.	+
-	Slide 6:	+	====Slide 7====
-	- Test Results	+	* Conclusions/Results!
		+	** Does it work/or not?
		+	** What we learned from constructing the device
		+	*** at the beginning, we tested all enzymes for functionality via test plasmid + running a gel - while doing this we learned that Xba1 works slower than EcoRI, SpeI, and PstI
		+	*** Team communication, allocate more time for testing in addition to construction/designate a test team
		+	** Limitations to the device
		+	*** can only detect temperatures in a small range
		+	*** does fluorescent protein stay? or decay quickly? ask Charles/Andy
		+	*** Experiments involving radiation - this thermometer cannot be used as the radiation will damage the DNA
		+	*** Expression of the reporter gene must be as sensitive as the activation of the transcription factor (for accuracy in timing events dependent on temperature and time)
		+	*** Future work that can be done to improve the device/design for next year
		+	*** investigate repressors etc that would be good for larger temperature ranges
		+	*** this would increase the application of the device allowing it to be another alternative to detecting temperature changes in biological systems.
-	- Tested the intermediary pBad/araC + LacI ts coding sequence (R0011) + reporter gene thoroughly	+	====Slide 8====
-	- We ensured that module 1 tests passed before continuing construction.	+	* Team Members
-		+
-	Slide 7	+	====Slide 9====
-	Conclusions/Results!	+	* Acknowledgements
-	- Does it work/or not?	+
-	- What we learned from constructing the device	+
-	- at the beginning, we tested all enzymes for functionality via test plasmid + running a gel - while doing this we learned that Xba1 works slower than EcoRI, SpeI, and PstI	+
-	- Team communication, allocate more time for testing in addition to construction/designate a test team	+
-	- Limitations to the device	+
-	- can only detect temperatures in a small range	+
-	- does fluorescent protein stay? or decay quickly? ask Charles/Andy	+
-	- Experiments involving radiation - this thermometer cannot be used as the radiation will damage the DNA	+
-	- Expression of the reporter gene must be as sensitive as the activation of the transcription factor (for accuracy in timing events dependent on temperature and time)	+
-	- Future work that can be done to improve the device/design for next year	+
-	- investigate repressors etc that would be good for larger temperature ranges	+
-	- this would increase the application of the device allowing it to be another alternative to detecting temperature changes in biological systems.	+
-		+
-		+
-	Slide 8:	+
-	- Team Members	+
-		+
-	Slide 9:	+
-	- Acknowledgements	+

---

Revision as of 04:01, 2 November 2006

The Powerpoint can be found here: Presentation

University_of_Toronto_2006

Contents 1 Presentation Script 1.1 Slide 1 1.2 Slide 2 1.3 Slide 3 1.4 Slide 4 1.5 Slide 5 1.6 Slide 6 1.7 Slide 7 1.8 Slide 8 1.9 Slide 9

Presentation Script

Slide 1

• Introduce the team (i.e. collaborative effort between UofT and UW) and mention the project we're working on.

Slide 2

• Explain what makes this thermometer unique from all other existing models as in other models may limitations to where/how they can be used
  • size of the species of interest may be too small
  • research experiments where chemicals, radiation may be toxic for a colony but temperature readings are needed

• A bio-synthetic thermometer may be a possible solution to overcome these obstacles.

• Applications:
  • use to detect small changes in temperature in very small organisms- we can do this by measuring the intensity of the colour emitted
  • experiments or places where a quantitative reading cannot be obtained (i.e a person can not physically be there to take the reading (in anaerobic conditions?)) or where air pressure
  • may also use this to monitor temperature of aquariums using zebra fish for example

Slide 3

• Explain diagrams

Slide 4

• Explain our approach: how we decided to ligate parts separately and build up to the final product
• Constructed many new parts
• Replaced our cI repressor system with tetracycline repressor (tetR), an analogous system to cI but less leaky and can control externally

Slide 5

• Testing Phase
  • Intro: - Testing was split into 3 modules.
    • Each module acts as a checkpoint for a particular milestone in the construction process.
    • After each module we can ensure a vital component of the construct is functional.
  • State the goal/purpose of each module and how it was executed.

Slide 6

• Test Results
  • Tested the intermediary pBad/araC + LacI ts coding sequence (R0011) + reporter gene thoroughly
  • We ensured that module 1 tests passed before continuing construction.

Slide 7

• Conclusions/Results!
  • Does it work/or not?
  • What we learned from constructing the device
    • at the beginning, we tested all enzymes for functionality via test plasmid + running a gel - while doing this we learned that Xba1 works slower than EcoRI, SpeI, and PstI
    • Team communication, allocate more time for testing in addition to construction/designate a test team
  • Limitations to the device
    • can only detect temperatures in a small range
    • does fluorescent protein stay? or decay quickly? ask Charles/Andy
    • Experiments involving radiation - this thermometer cannot be used as the radiation will damage the DNA
    • Expression of the reporter gene must be as sensitive as the activation of the transcription factor (for accuracy in timing events dependent on temperature and time)
    • Future work that can be done to improve the device/design for next year
    • investigate repressors etc that would be good for larger temperature ranges
    • this would increase the application of the device allowing it to be another alternative to detecting temperature changes in biological systems.

Slide 8

• Team Members

Slide 9

• Acknowledgements