Presentation
From 2006.igem.org
The Powerpoint can be found here: Presentation
Contents |
Presentation Script
Slide 1
- Introduce the team (i.e. collaborative effort between UofT and UW) and mention the project we're working on.
Slide 2
- Explain what makes this thermometer unique from all other existing models as in other models may limitations to where/how they can be used
- size of the species of interest may be too small
- research experiments where chemicals, radiation may be toxic for a colony but temperature readings are needed
- A bio-synthetic thermometer may be a possible solution to overcome these obstacles.
- Applications:
- use to detect small changes in temperature in very small organisms- we can do this by measuring the intensity of the colour emitted
- experiments or places where a quantitative reading cannot be obtained (i.e a person can not physically be there to take the reading (in anaerobic conditions?)) or where air pressure
- may also use this to monitor temperature of aquariums using zebra fish for example
Slide 3
- Explain diagrams
Slide 4
- Explain our approach: how we decided to ligate parts separately and build up to the final product
- Constructed many new parts
- Replaced our cI repressor system with tetracycline repressor (tetR), an analogous system to cI but less leaky and can control externally
Slide 5
- Testing Phase
- Intro: - Testing was split into 3 modules.
- Each module acts as a checkpoint for a particular milestone in the construction process.
- After each module we can ensure a vital component of the construct is functional.
- State the goal/purpose of each module and how it was executed.
- Intro: - Testing was split into 3 modules.
Slide 6
- Test Results
- Tested the intermediary pBad/araC + LacI ts coding sequence (R0011) + reporter gene thoroughly
- We ensured that module 1 tests passed before continuing construction.
Slide 7
- Conclusions/Results!
- Does it work/or not?
- What we learned from constructing the device
- at the beginning, we tested all enzymes for functionality via test plasmid + running a gel - while doing this we learned that Xba1 works slower than EcoRI, SpeI, and PstI
- Team communication, allocate more time for testing in addition to construction/designate a test team
- Limitations to the device
- can only detect temperatures in a small range
- does fluorescent protein stay? or decay quickly? ask Charles/Andy
- Experiments involving radiation - this thermometer cannot be used as the radiation will damage the DNA
- Expression of the reporter gene must be as sensitive as the activation of the transcription factor (for accuracy in timing events dependent on temperature and time)
- Future work that can be done to improve the device/design for next year
- investigate repressors etc that would be good for larger temperature ranges
- this would increase the application of the device allowing it to be another alternative to detecting temperature changes in biological systems.
Slide 8
- Team Members
Slide 9
- Acknowledgements
