Swimmy Bacteria : Chiba 2006

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=How it works=
=How it works=
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its kind of simple...<br />
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--under construction--
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to stop the e.coli by light, we needed a part that stop the e.coli, and combine it with a light receptor that UCSF made it last year.
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We've found out, from the paper(..see refrences) the Chemoreceptor that fix the ''E.coli'' tumble.
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[[Image:Devices.jpg]]
[[Image:Devices.jpg]]
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Revision as of 15:04, 29 October 2006

Contents

Concept

Swimmy Bacteria -- the E.coli freeze and gather when they are caught by red light.
conceptSwimmy
red light on -> stop
red light off -> move
Control the E.coli's movement with light!

How it works

--under construction--

to stop the e.coli by light, we needed a part that stop the e.coli, and combine it with a light receptor that UCSF made it last year.


We've found out, from the paper(..see refrences) the Chemoreceptor that fix the E.coli tumble.

Devices.jpg
pathway

Members

Chiba 2006 team あ

Experiments

light cannon

  • 10/15 we wrote a picture at the solid media.(not finished yet) also we changed the arabinose concentration at the liquid media.
  • 10/14 the Fluorescent lamp was the best light. we could see the on/off clearly in the solid media. we found that the thin media is better than the thicker one.
  • 10/11 we changed the arabinose & S-gal's concentration & the light sorce to find the best condition.

swimmy

  • 10/15 tried the capiraly assay to see chemotaxis. also we washed the e.coli and observed it with the microscope(but it was kind of hard)
  • 10/11 we're finding the suitable media to observe the E.coli's movement(chemotaxis).
  • 8/23 we made the tsr gene parts by PCR & discussed about the locked Tsr mutants.

Todo List

Referenses

Locked Tsr Mutants

  • A.Bren and M.Eisenbach (2000) -- How Signals Are Heard during Bacterial Chemotaxis: Protein-Protein Interactions in Sensory Signal Propagation (J.Bacteriol.)
  • P.Ames and J.S.Parkinson (1988) -- Transmembrane Signaling by Bacterial Chemoreceptors: E.coli Transducers with Locked Signal Output (Cell)
  • P.Ames and J.S.Parkinson (1994) -- Constitutively Signaling Fragments of Tsr, the Escherichia coli Serine Chemoreceptor (J.Bacteriol.)


Experiments

  • Microscope -- Real-time imaging of fluorescent flagellar filaments.J.bacteriol.2000
  • Chemotaxis Assay -- J.Adler -- A Method for Measuring Chemotaxis and Use of the Method to Determine Optimum Conditions for Chemotaxis by Escherichia coli
  • M.K.Slocum and J.S.Parkinson (1985) -- Genetics of Methyl-Accepting Chemotaxis Proteins in Escherichia coli: Null Phenotypes of the tar and tap Genes (J.Bacteriol.)
  • J.S.Parkinson (1976) --- cheA, cheB, and cheC of Escherichia coli and Their Role in Chemotaxis (J.Bacteriol.)

Last Update

  • 10/13 todo list
  • 10/11 swimmy & gene pathway
  • 8/23 refrences
  • 8/7 concept&members --maiko


note

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