From 2006.igem.org

Contents 1 Concept 2 How it works 3 Members 4 Experiments 4.1 light cannon 4.2 swimmy 5 Todo List 6 Referenses 6.1 Locked Tsr Mutants 6.2 Experiments 7 Last Update

Concept

Swimmy Bacteria -- the E.coli freeze and gather when they are caught by red light.

red light on -> stop
red light off -> move
Control the E.coli's movement with light!

How it works

its kind of simple...

Members

Chiba 2006 team あ

• Youhei Tashiro
• Tomoki Morijiri
• Toru Karasawa
• Maiko Furubayashi

Experiments

light cannon

• 10/15 we wrote a picture at the solid media.(not finished yet) also we changed the arabinose concentration at the liquid media.
• 10/14 the Fluorescent lamp was the best light. we could see the on/off clearly in the solid media. we found that the thin media is better than the thicker one.
• 10/11 we changed the arabinose & S-gal's concentration & the light sorce to find the best condition.

swimmy

• 10/15 tried the capiraly assay to see chemotaxis. also we washed the e.coli and observed it with the microscope(but it was kind of hard)
• 10/11 we're finding the suitable media to observe the E.coli's movement(chemotaxis).
• 8/23 we made the tsr gene parts by PCR & discussed about the locked Tsr mutants.

Todo List

• memo
• 10/18
• 10/16
• 10/15
• 10/14
• 10/13

Referenses

Locked Tsr Mutants

• A.Bren and M.Eisenbach (2000) -- How Signals Are Heard during Bacterial Chemotaxis: Protein-Protein Interactions in Sensory Signal Propagation (J.Bacteriol.)
• P.Ames and J.S.Parkinson (1988) -- Transmembrane Signaling by Bacterial Chemoreceptors: E.coli Transducers with Locked Signal Output (Cell)
• P.Ames and J.S.Parkinson (1994) -- Constitutively Signaling Fragments of Tsr, the Escherichia coli Serine Chemoreceptor (J.Bacteriol.)

Experiments

• Microscope -- Real-time imaging of fluorescent flagellar filaments.J.bacteriol.2000
• Chemotaxis Assay -- J.Adler -- A Method for Measuring Chemotaxis and Use of the Method to Determine Optimum Conditions for Chemotaxis by Escherichia coli
• M.K.Slocum and J.S.Parkinson (1985) -- Genetics of Methyl-Accepting Chemotaxis Proteins in Escherichia coli: Null Phenotypes of the tar and tap Genes (J.Bacteriol.)
• J.S.Parkinson (1976) --- cheA, cheB, and cheC of Escherichia coli and Their Role in Chemotaxis (J.Bacteriol.)

Last Update

• 10/13 todo list
• 10/11 swimmy & gene pathway
• 8/23 refrences
• 8/7 concept&members --maiko