Testing Results
From 2006.igem.org
Overview
We decided to integrate check-points into our construction plan, in order to test intermediary part functionality.
There are three basic modules:
- Module 1: Arabinose inducible pBad/araC promoter (I0500) produces temperature-sensitive LacI (LacI ts), which then, upon dimerization, represses the IPTG inducible LacI+ pL promoter (R0011) resulting in a decrease in GFP (E0240)/mRFP (I13507)
- Module 2: In Module 1, insert DNA sequence coding for 434 cI protein instead of the reporters (GFP/mRFP) and then add Prm+ promoter (I12006), which is repressed by 434 cI and add GFP
- Module 3: Take Module 3 and add mRFP (J04450)
