The What's and How's

From 2006.igem.org

Revision as of 20:16, 13 September 2006

• What is the list of DNA parts that we will need for the first stage, second stage, entire project?
  
• ERIN and SABRIYA Which DNA parts exist in the registry?Answer1More Parts
  
• ERIN and SABRIYAWhich DNA parts will have to be designed? Will they be synthesized or produced by PCR?
  

loxP sites Hin/Hix CRE gene Degradation Tags

• Which plasmids will be used from the registry?
  
• Where would biobricks be located?
  
• MALCOLM What is the protocol for assembly for the first stage (restriction digestions, ligations, transformations)?
  

1. [Common molecular reagents]
2. [Building dsDNA with Oligos]
3. [Setting up PCR mixtures]
4. [PCR and Mg²⁺ concentration]
5. [Pouring an agarose gel]
6. [Calculate MWs]
7. [Digest DNA with restriction enzymes]
8. Double Digest Guide
9. [Clean DNA (short protocol)]
10. [1kb MW markers]
11. [Shrimp Alkaline Phosphatase]
12. [Gel Purification]
13. [Ligation Protocol]
14. [Transformation] [Short version]
15. [Promega miniprep]