The What's and How's
From 2006.igem.org
- What is the list of DNA parts that we will need for the first stage, second stage, entire project?
- ERIN and SABRIYA Which DNA parts exist in the registry?Answer1More Parts
- ERIN and SABRIYAWhich DNA parts will have to be designed? Will they be synthesized or produced by PCR?
loxP sites Hin/Hix CRE gene Degradation Tags
- Which plasmids will be used from the registry?
- Where would biobricks be located?
- MALCOLM What is the protocol for assembly for the first stage (restriction digestions, ligations, transformations)?
- [Common molecular reagents]
- [Building dsDNA with Oligos]
- [Setting up PCR mixtures]
- [PCR and Mg2+ concentration]
- [Pouring an agarose gel]
- [Calculate MWs]
- [Digest DNA with restriction enzymes]
- Double Digest Guide
- [Clean DNA (short protocol)]
- [1kb MW markers]
- [Shrimp Alkaline Phosphatase]
- [Gel Purification]
- [Ligation Protocol]
- [Transformation] [Short version]
- [Promega miniprep]