The What's and How's

From 2006.igem.org

• What is the list of DNA parts that we will need for the first stage, second stage, entire project?
  
• ERIN and SABRIYA Which DNA parts exist in the registry?Answer1More Parts
  
• ERIN and SABRIYAWhich DNA parts will have to be designed? Will they be synthesized or produced by PCR?
  

loxP sites Hin/Hix CRE gene Degradation Tags

• Which plasmids will be used from the registry?
  
• Where would biobricks be located?
  
• MALCOLM What is the protocol for assembly for the first stage (restriction digestions, ligations, transformations)?
  

1. [Common molecular reagents]
2. [PCR and Mg²⁺ concentration]
3. [Pouring an agarose gel]
4. [Calculate MWs]
5. [Digest DNA with restriction enzymes]
6. [1kb MW markers]
7. Shrimp alkaline phosphatase URL to come
8. Ligation URL to come
9. [Transformation] [Short version]
10. Promega miniprep URL to come