Toronto

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== Project Overview ==
== Project Overview ==
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[http://individual.utoronto.ca/nazareth/wiki.html iGEM Project Overview]
 
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Here’s an overview of the project.  Currently the write up is an external link to an html, eventually it will be converted to wiki form to allow easier modification.
 
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[http://individual.utoronto.ca/nazareth/Model.pdf Cell-See-Us Thermometer Model]
 
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Here’s a model of the Cell-See-Us Thermometer, including the governing system of differential equations.
 
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[http://individual.utoronto.ca/nazareth/ModelResults.pdf Cell-See-Us Thermometer Model Results]
 
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And here are the results of the model, after values for the constants were plugged in.
 
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The original designs for the temperature sensor, parts BBa_J11014 and BBa_J11018 are available below, along with modeling data. (These files are jpegs, and considerably smaller than the pdfs above).
 
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[http://www.math.uwaterloo.ca/~mscott/BBa_J11014.jpg Temperature sensor]
 
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[http://www.math.uwaterloo.ca/~mscott/BBa_J11018.jpg Temperature sensor with positive feedback]
 
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The design has since been modified to accommodate minimal parts, called BBa_J11021 and BBa_J11022. The model results for these new designs will be posted shortly.
The design has since been modified to accommodate minimal parts, called BBa_J11021 and BBa_J11022. The model results for these new designs will be posted shortly.
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== Message Board==
== Message Board==

Revision as of 20:59, 5 November 2005

Welcome to the Blue Water Wiki


Project Overview

The design has since been modified to accommodate minimal parts, called BBa_J11021 and BBa_J11022. The model results for these new designs will be posted shortly.

Message Board

Good news, there is an E coli strain availabe that contains Tn10 inserted into LacZ, meaning it is lacZ- and Tetracycline sensitive.

about the Tet operon and Tn10 http://www.biomedcentral.com/1471-2180/5/1 http://www.biomedcentral.com/1471-2199/3/13

-Hannah

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UPdate ~ 12 Oct 2005

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15:54, 3 Oct 2005 (EDT)15:54, 3 Oct 2005 (EDT)~~ Hi everyone, I made a biobrick version of my system, http://parts2.mit.edu/r/parts/partsdb/view.cgi?part_id=6153 It is part # BBa_J11030 I hope all the parts are available... Also very important NOTE that this system MUST MUST MUST be in LacZ- bugs! So lets try and get a hold of those if we want to make my system

Hannah

[http://pubs.acs.org/cgi-bin/archive.cgi/chreay/2000/100/i07/pdf/cr990115p.pdf Genetically engineered whole-cell sensing systems: coupling biological recognition with reporter genes.]

Chem Rev. 2000 Jul 12;100(7):2705-38. Daunert S, Barrett G, Feliciano JS, Shetty RS, Shrestha S, Smith-Spencer W.

Discusses important biosensing systems, mainly in E. coli.

On another note, currently if anyone wants to post files (Powerpoint presentations, papers, etc.) the file must be available online somewhere and linked to from this Wiki. If you need a file to be posted online, feel free to email it to me at emanuelnazareth@hotmail.com and I'll get back to you with how to link to it.

~ Emanuel

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