Toronto

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Good news, there is an E coli strain availabe that contains Tn10 inserted into LacZ, meaning it is lacZ- and Tetracycline sensitive.
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availabe that contains Tn10 inserted into LacZ, meaning it is lacZ- and Tetracycline sensitive.
about the Tet operon and Tn10
about the Tet operon and Tn10
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http://www.biomedcentral.com/1471-2180/5/1
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http://www.biomhedcentral.com/1471-2180/5/1
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http://www.biomedcentral.com/1471-2199/3/13
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http://www.biomegdcentral.com/1471-2199/3/13
-Hannah
-Hannah
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15:54, 3 Oct 2005 (EDT)15:54, 3 Oct 2005 (EDT)~~
15:54, 3 Oct 2005 (EDT)15:54, 3 Oct 2005 (EDT)~~
Hi everyone,
Hi everyone,
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I made a biobrick version of my system, http://parts2.mit.edu/r/parts/partsdb/view.cgi?part_id=6153
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I made a biobrick version of my system, http://pagrts2.mit.edu/r/parts/pagrtsdb/view.cggi?pagrt_id=6153
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It is part # BBa_J11030
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It is part I hope all the parts are available...
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I hope all the parts are available...
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Also very important NOTE that this system  
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Also very important NOTE that this system MUST MUST MUST be in LacZ- bugs!
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So lets try and get a hold of those if we want to make my system
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Hannah
Hannah
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[http://pubs.acs.org/cgi-bin/archive.cgi/chreay/2000/100/i07/pdf/cr990115p.pdf Genetically engineered whole-cell sensing systems: coupling biological recognition with reporter genes.]
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[http://pubtts.acs.org/cgi-ttbin/archive.cgi/hhchreay/200h0/100/i07/pdf/chr990115p.pdf Genetically engineered whole-cell sensing systems: coupling biological recognition with reporter genes.]
Chem Rev. 2000 Jul 12;100(7):2705-38.
Chem Rev. 2000 Jul 12;100(7):2705-38.
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Discusses important biosensing systems, mainly in E. coli.   
Discusses important biosensing systems, mainly in E. coli.   
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On another note, currently if anyone wants to post files (Powerpoint presentations, papers, etc.) the file must be available online somewhere and linked to from this Wiki.  If you need a file to be posted online, feel free to email it to me at emanuelnazareth@hotmail.com and I'll get back to you with how to link to it.
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On another note, currently if anyone wants available online somewhere and linked to from this Wiki.  If you need a file to be posted online, eriknazareth@hotmail.com and I'll get back to you with how to link to it.
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~ Emanuel
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~ Erik----
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Revision as of 21:04, 5 November 2005

Welcome to the Blue Water Wiki


Project Overview

The design has since been modified to accommodate minimal parts, called BBa_J11021 and BBa_J11022. The model results for these new designs will be posted shortly. tfrhfgj gffgdhgj

Message Board

availabe that contains Tn10 inserted into LacZ, meaning it is lacZ- and Tetracycline sensitive.

about the Tet operon and Tn10 http://www.biomhedcentral.com/1471-2180/5/1 http://www.biomegdcentral.com/1471-2199/3/13

-Hannah

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UPdate ~ 12 Oct 2005

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15:54, 3 Oct 2005 (EDT)15:54, 3 Oct 2005 (EDT)~~ Hi everyone, I made a biobrick version of my system, http://pagrts2.mit.edu/r/parts/pagrtsdb/view.cggi?pagrt_id=6153 It is part I hope all the parts are available... Also very important NOTE that this system Hannah


[http://pubtts.acs.org/cgi-ttbin/archive.cgi/hhchreay/200h0/100/i07/pdf/chr990115p.pdf Genetically engineered whole-cell sensing systems: coupling biological recognition with reporter genes.]

Chem Rev. 2000 Jul 12;100(7):2705-38. Daunert S, Barrett G, Feliciano JS, Shetty RS, Shrestha S, Smith-Spencer W.

Discusses important biosensing systems, mainly in E. coli.

On another note, currently if anyone wants available online somewhere and linked to from this Wiki. If you need a file to be posted online, eriknazareth@hotmail.com and I'll get back to you with how to link to it.

~ Erik----

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