From 2006.igem.org
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| - | # Thaw the compentent cells by warming in between hands. Cells should be used immediately after being thawed.
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| - | # Add 1μg of DNA immediately to the tube containing the cells.
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| - | #Gently swirl to mix.
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| - | #Place DNA on ice for 10 minutes.
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| - | #Heat shock cells by placing into a 42°C water bath for two minutes.
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| - | # Add 1mL LB no amp medium to each tube.
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| - | # Incubate for one hour at 37°C.
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| - | #Spin cells for 2 min and resuspend the cells in 100μl of LBamp medium.
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| - | # Place μl of transformation on LB/ampicillin-containing plates.
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| - | # Spread over the surface of the plate until dry.
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| - | # Once the plate is dry incubate for 12-16 hours at 37°C.
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| - | # Any remaining transformation mixture can be stored at 4°C and used for subsequent plating.
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Latest revision as of 17:14, 8 June 2006
