University of Michigan 2006

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(Advisors)
(From Design to Manufacturing)
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  '''2. Recursive Landing Pads''' <br>
  '''2. Recursive Landing Pads''' <br>
-
     An innovative method for inserting genes and DNA sequences onto E. coli's chromosome.
+
     A super-duper-awesomely innovative method for inserting genes and DNA sequences onto E. coli's chromosome  
 +
      (that Steve can explain much better than I can, and hopefully he will as soon as he gets back from class).
  '''3. Modeling Oscillatory Behavior of Clocks '''<br>
  '''3. Modeling Oscillatory Behavior of Clocks '''<br>
Line 64: Line 65:
     primarily examining the ideal conditions for generating oscillations.
     primarily examining the ideal conditions for generating oscillations.
-
  '''4. Increasing Kinetic Order Through the Use of Sinks '''<br>
+
  '''4. Experimental Manipulation of Lac Operon Kinetic Order '''<br>
-
     A promising method for raising kinetic order in genetic systems to increase chances for oscillations.
+
     [[Image:XsomesOp.gif]]
 +
    Experimental assays of strains and plasmids with Op sinks were performed.
 +
    Strains were created by inserting sinks on chromosome.
 +
    Landing pads included rbs, glnK and trp regions.
 +
    Results showed the following:
 +
      - Sinks on plasmid raise basal level of lacZ expression;
 +
      - Sinks on chromosome raise kinetic order;
 +
      - One strain in particular was of significant interest.
 +
    Findings can be used for "tuning" genetic systems (clocks, oscillators, etc).
=Links=
=Links=

Revision as of 22:03, 30 October 2006

We're the iGEM team from the [http://www.umich.edu/ University of Michigan].


Contents

News

  • 2006.03.15 We opened our wiki page!

Organisation

Mailing Address

Peter Woolf
Department of Chemical Engineering
2300 Hayward St.
3074 H.H. Dow Building
Ann Arbor, MI 48109-2136

People

Students

Not pictured here:

Advisors

timeline

tasks

  • Update Wiki

Our Project

From Design to Manufacturing

1. Paul-Tron 2k 
An integrative prgramming environment for designing and testing genetic systems.
2. Recursive Landing Pads 
A super-duper-awesomely innovative method for inserting genes and DNA sequences onto E. coli's chromosome (that Steve can explain much better than I can, and hopefully he will as soon as he gets back from class).
3. Modeling Oscillatory Behavior of Clocks 
A look at the parameter space of clocks and genetic oscillating devices, primarily examining the ideal conditions for generating oscillations.
4. Experimental Manipulation of Lac Operon Kinetic Order 
XsomesOp.gif Experimental assays of strains and plasmids with Op sinks were performed. Strains were created by inserting sinks on chromosome. Landing pads included rbs, glnK and trp regions. Results showed the following: - Sinks on plasmid raise basal level of lacZ expression; - Sinks on chromosome raise kinetic order; - One strain in particular was of significant interest. Findings can be used for "tuning" genetic systems (clocks, oscillators, etc).

Links

[http://synthbio.engin.umich.edu/wiki/ University of Michigan Team Wiki]

Miscellani

UMlogo.gif
Personal tools
Past/present/future years