University of Texas 2006
From 2006.igem.org
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==Light Controlled Chemotaxis== | ==Light Controlled Chemotaxis== | ||
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==Photopolymerization== | ==Photopolymerization== | ||
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+ | ==Favorite Parts== | ||
+ | *[http://partsregistry.org/Part:BBa_I15008 I15008]<br> | ||
+ | *[http://partsregistry.org/Part:BBa_I15009 I15009]<br> | ||
+ | *[http://partsregistry.org/Part:BBa_I15010 I15010]<br> | ||
+ | *[http://partsregistry.org/Part:BBa_I15022 I15022]<br> | ||
Revision as of 03:55, 2 November 2006
The UT Austin team is:
- Aaron Chevalier
- Eric Davidson
- Jeff Tabor
- Laura Lavery
- Matt Levy
- Zack Booth Simpson
- Bryan Kaehr
Advisors:
Contents |
Previous work: Bacterial Photography
An obvious hurdle in the implementation of this system was genetically encoding light detection in the naturally blind E.coli. To accomplish this, we used a synthetic part engineered in the Voigt lab. This part, Cph8, (I15010) is an engineered fusion between a cyanobacterial light sensing phytochrome (Cph1) and an E.coli transmembrane histidine kinase, (EnvZ). 660nm light causes an isomerization in the Cph1 domain of the chimera which inactivates the histidine kinase acitity of EnvZ. When EnvZ is inhibited, a phosphorelay cascade which activates transcription from the OmpC promoter R0082) and inhibits transcription from the OmpF promoter (R0084). We then demonstrated that when this system is expressed in E.coli, it is possible to transform each cell on an agar surface into a decision making pixel capable of reporting whether it is in the light or dark. The community of cells is therefore capable of genetically reproducing a light image.
Current work: Edge detector
Light Controlled Chemotaxis
Photopolymerization
Favorite Parts