Penn State University 2006

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The bacterial relay race takes advantage of an ability to control cellular motility using inducible promoters such as those involved in nutrient catabolism or quorum sensing. “Receiver” bacteria move in response to small-molecule signals either added to the system or originating from motile, “sender” strains. The most significant challenges relating to this project stem from difficulties of tightly controlling the target motility gene motB. Low levels of motB expression result in system failure (constitutive motility), and resolving this issue is essential to developing reliable modular systems that are the hallmark of synthetic biology.

>People

Undergraduate Team

  • [http://openwetware.org/wiki/IGEM:PennState/2006Jon Badalamenti]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Lucien Weiss]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Chris Buckno]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Joel Q. Grim]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Ralph Sandoval]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Barbara Andersen]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Jen Dunning]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Natalie Shewski]
  • [http://openwetware.org/wiki/IGEM:PennState/2006John Bigus]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Aleksandr Lewicki]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Elizabeth Morin]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Yan Shipelskiy]

Affiliated Grad Students

  • [http://openwetware.org/wiki/IGEM:PennState/2006Steve Walker]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Deepti Tanjore]

Faculty

  • [http://openwetware.org/wiki/IGEM:PennState/2006Tom Richard]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Pat Cirino]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Will Hancock]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Darryl Farber]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Ming Tien]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Paul Weiss]

Graduated Team Members

  • [http://openwetware.org/wiki/IGEM:PennState/2006Lane J. Weaver]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Tony Tascone]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Steve Neuman]

Photos

  • [http://openwetware.org/wiki/IGEM:PennState/2006Lab Photos]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Team Member Photos]



>Project

Publications

  • [http://openwetware.org/wiki/IGEM:PennState/2006Synthetic Sports: A Bacterial Relay Race]

Protocols

  • [http://openwetware.org/wiki/IGEM:PennState/2006Cloning]
    • [http://openwetware.org/wiki/IGEM:PennState/2006Preparing chemically competent cells]
    • [http://openwetware.org/wiki/IGEM:PennState/2006Growing Parts]
    • [http://openwetware.org/wiki/IGEM:PennState/2006Restriction]
    • [http://openwetware.org/wiki/IGEM:PennState/2006Ligation]
    • [http://openwetware.org/wiki/IGEM:PennState/2006Transformation]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Plate-reading]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Motility-assays]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Microchannel]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Strain Construction]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Inventory]
  • [http://openwetware.org/wiki/IGEM:PennState/2006DNA Gel]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Growth Media]
  • [http://openwetware.org/wiki/IGEM:PennState/2006LB Plates]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Eiken Agar Plates]

Progress

  • [http://openwetware.org/wiki/IGEM:PennState/2006Submitted Registry Parts]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Strain-Construction Progress]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Microchannels results]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Motility results]

Background

Links

  • iGEM on OpenWetWare
  • [http://partsregistry.org Parts registry]
  • [http://2007.igem.org iGEM wiki]
  • [http://2006.igem.org/wiki/index.php/Penn_State_2005 Penn State iGEM 2005]
  • [http://2006.igem.org/wiki/index.php/Penn_State_University_2006 Penn State iGEM 2006]

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