Plasmid Prep

From 2006.igem.org

You do not have permission to edit this page, for the following reason:

You can view and copy the source of this page:

Do plasmid prep on pellets in three tubes labelled "GFP TE" in bottom of left fridge. They are from overnight culture of plasmid I13522 (pTet-GFP). Combine the eluted plasmid from all the preps into one tube and measure the yield as follows: # turn on UV spec and blank with H2O # measure A260 of 4 ul plasmid + 76 ul H2O # this measurement gives the concentration in ug/ul, multiply by 1000 to give ng/ul Plasmid prep was done by Brad and Eric (Followed plasmid prep protocol), and I (Kelly) jumped in to help with the A260 readings. We obtained 71 ng/uL.

Return to Plasmid Prep.