Phase 2: Plasmid Construction and Bacterial Transformations

From 2006.igem.org

You do not have permission to edit this page, for the following reason:

You can view and copy the source of this page:

'''1. Isolated ptetRBS and pSB1A3 plasmids from BioBricks''' ::*Attached tet promoter with Ribosome Binding Site to GFP gene, transformed XL-1 ''E. coli'' and observed positive expression <br> <br> '''2. Ligated ComQX/P/A and Tsr fragments from Phase 1 into pSB1A3 plasmids''' ::*Used SpeI and XbaI restriction sites ::*Verified transformation for all fragments and sequenced genes (results positive in all cases). <br> <br> '''3. Significance: ::*We now have all the fundamental components for the project ahead ::*Can proceed in constructing the hybrid quorumtaxis receptor using ComP and Tsr genes ::*Will need to perform quick-change mutagenesis for ComA to remove internal SpeI site

Return to Phase 2: Plasmid Construction and Bacterial Transformations.