Davidson Parts

'''''Hin gene: Part BBa_J31000'''''

Primers for Hin gene the 72nd base was mutated from a "T" to a "C" in order to remove a naturally occuring SpeI site. The mutatation is in '''bold'''.

'''Forward primer'''

5’ gcattctagaatggctactattgggtatattcgggtgtcaacaattgaccaaaatatcgatttacagcgtaatgcgcttac'''C'''agtgcaaattg 3’

'''Reverse primer'''

5’ atgcctgcaggcggccgcactagtttaattcattcgtttttttatac  3’

'''''Hin gene with LVA degredation tag'''''

Will have the following primers synthesized so that the Hin gene has a LVA degredation tag.

'''Forward Primer'''
*Consists of a <font color='green'>BioBrick prefix</font color>, <font color='blue'>four base pairs from plasmid pSB1A2 [http://partsregistry.org/Part:pSB1A2]</font color>, and<font color='orange'> the first three base pairs of the Hin gene</font color>
<font face="courier new"><font color='blue'>TCTG</font color><font color='green'>GAATTCGCGGCCGCATCTAGAG</font color><font color='orange'>ATG</font color></font face>

'''''Reverse Primer'''''
*Consists of the <font color='green'>Bio Brick suffix</font color>,<font color='red'> Stop codon</font color>, <font color='purple'>LVA tag</font color> and the ,<font color='orange'>last 20 base pairs of the Hin gene</font color> (without the stop codon)

<font face="courier new"><font color='green'>CTGCAGGCGGCCGCTACTAGT</font color><font color='red'>ATT</font color><font color='purple'>AAGCTACTAAAGCGTAGTTTTCGTCGTTTGCAGC</font color><font color='orange'>ATTCATTCGTTTTTTTATAC</font color></font face>


[[Recombinational Enahancer: Part BBa_J3101]]

'''''Recombinational Enhancer: Part BBa_J3101'''''

These are the oligos that we ordered to make the recombinational enhancer.

AATTCGCGGCCGCTTCTAGATTCGGGTGTCAACAATTGACCAAAATAT

CGATTTACAGCGTAATGCGCTTTCTAGTGCAAATTGTGACCGC

ATTTTACTAGTTGCGGCCGCCTGCA

GGCGGCCGCAACTAGTAAAATGCGGTCACAATTTGCACTAGAA

AGCGCATTACGCTGTAAATCGATATTTTGGTCAATTGTTGACACCCGAATCTAGAAGCGGCCGCG