Brown:Journal club/5.02.06

From 2006.igem.org

You do not have permission to edit this page, for the following reason:

You can view and copy the source of this page:

Minutes Below is a list of the team members and what each of them is responsible for doing. Those in bold are new additions from the old to-do list. Victoria – faculty liaison, '''faculty mentoring group (when to meet), alumni database (biotech), contact entrepreneurship program''' Kara – MCB, Navartis, Industry funding Meghan – Pres. Funding, website, future planning Angela – funding, minutes John – lead, wiki, funding, department fundraising, future planning Jesse – funding, equipment Brendan – PR, CS, MCB, Sorin (contact), goldman sachs? Peter G – wiki, CS, redo to-do list Annie – research, courses and workshops (modeling, PCR, cloning, bio bricks, etc.) Jamie G – magnetic bacteria Hiyato – free radical reporter Ana – magnetic bacteria, contact Sheldon about alumni mag. Azeem – research, cell counter, monthly planning Jason – free radical reporter, equipment needed Jamie L – alumni data base (biotech), contact entrepreneurship program - we will have a meeting the weekend after this one CS funding update: - they wanted to know if we had contacted MCB - go and meet (John and Peter) Sri presents on BacteriophageT7 - phage goes into a cell, copied and the exits - T7 RNA polymerase encoded by gene 1 - 19 mapped essential genes, new ones named under existing structure, about 50 total - self contained during infection - host’s RNA polymerase is the motor which brings in the phage’s DNA - process takes 10-15 min, infection completion takes 15-20 mins - investigate if there is reason for genome’s layout - T7 gene expression - create model for gene expression - measure mRNA levels - track polymerases as they transcribe DNA - take 100’s to get population data - data collected by real time PCR - can see when PCR products come up - results: model is completely wrong - early mRNA is degraded - adjust model, rates fit better - try to encode model onto DNA - see paper from last week - problems with physical rearrangement - overlaps – how important are they? - cloned as much as could with ecoli, then used PCR fusion techniques - produce a phage that can survive - then clone within the phage - alternative cloning vehicles? - Thinking of yeast - only weak promoters see point mutations - open reading frames: some known, unknown/unimportant - T7.1 - Better control, inference about data easier - Eukaryotes have fewer overlaps b/c of larger genome - Being done with yeast

Return to Brown:Journal club/5.02.06.