Brown:Equipment needed

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{| border=1 cellspacing=0 cellpadding=5
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{| border=1 cellspacing=0 cellpadding=1
 +
|-
 +
|
 +
| type/size
 +
| quantity
 +
| Have(x)
 +
| Location
 +
| Comments
|-
|-
| '''Equipment'''
| '''Equipment'''
-
|  
+
|
-
|-
+
|
 +
|-  
| freezers/fridges
| freezers/fridges
|  
|  
|  
|  
 +
|-
|  
|  
-
|-
+
| -80°C
-
| incubator  
+
| 1
-
|-
+
| we have space in a -80
 +
| JWW basement
 +
|-
 +
|
 +
| -20°C
 +
| 1
 +
| yes
 +
| JWW228
 +
|-
 +
|
 +
| 4°C
 +
| 1
 +
| yes
 +
| JWW228
 +
|-  
 +
| incubator (what kind?)
 +
|
 +
| 1
 +
| might work (heh)
 +
| JWW228
 +
|-
 +
| pH meter
 +
|
 +
| 1
 +
|
 +
|-  
| hot bath
| hot bath
-
|-
+
|
 +
| 1
 +
| we have access
 +
| JWW218
 +
|-  
| cold bath
| cold bath
-
|-
+
|
 +
| 1
 +
|-  
| autoclave
| autoclave
-
|-
+
|
-
| cell shaker/incubator
+
| 1
-
|-
+
| we have access
 +
|JWW 4th Floor
 +
|
 +
 
 +
|-  
 +
| cell shaker inside 37 degree incubator
 +
|
 +
| 1
 +
| we have acess
 +
| JWW 3rd floor
 +
|-  
| source of ddH2O
| source of ddH2O
-
|-
+
|
 +
| 1
 +
|-  
| hot plates w/ stirring
| hot plates w/ stirring
-
|-
+
|
 +
| 2
 +
|-  
| stir bars (set)
| stir bars (set)
-
|-
+
|
 +
| 1
 +
|-  
| PCR thermocycler
| PCR thermocycler
-
|-
+
|
 +
| 1
 +
|-  
| microcentrifuge (w/temp cntrl.)
| microcentrifuge (w/temp cntrl.)
-
|-
+
| temp. cntrl not essential
 +
| 1
 +
|-  
| centrifuge  
| centrifuge  
-
|-
+
| 50mL and 15mL
 +
| 1
 +
| yes - 15 mL
 +
| very old, JWW228
 +
|-  
| Electrophoresis gel set
| Electrophoresis gel set
-
|-
+
|
 +
| 4
 +
|-  
| Gel Power source
| Gel Power source
-
|-
+
|
 +
| 1
 +
|-  
| UV Gel viewer
| UV Gel viewer
-
|-
+
|
 +
| 1
 +
|-  
| UV Gel camera
| UV Gel camera
-
|-
+
|
 +
| 1
 +
|-  
| Pipettemen
| Pipettemen
|  
|  
|  
|  
 +
|-
|  
|  
-
|-
+
| 20μL
 +
| 4
 +
|-
 +
|
 +
| 200μL
 +
| 4
 +
|-
 +
|
 +
| 1000μL
 +
| 4
 +
|-  
| Electronic pipettor
| Electronic pipettor
 +
| can use 1-25mL pipettes
 +
| 1
 +
|-
 +
| Manual pipettors / bulbs
 +
|
 +
| 2
|-
|-
-
| bunsen burners
+
| bunsen burner
-
|-
+
|
 +
| 2
 +
|-  
| sparkers
| sparkers
-
|-
+
|
 +
| 2
 +
|-  
| wire loop
| wire loop
-
|-
+
| to pick a bac clone
 +
| 3
 +
|-  
| metal loop
| metal loop
 +
| to spread bacteria
 +
| 3
 +
|- 
 +
| '''Glassware'''
 +
|
 +
|
 +
|-
 +
| Bottles
|  
|  
-
|-
 
-
| Glassware
 
|  
|  
-
|-
+
|-  
-
| Bottles
+
|  
|  
 +
| 1L
 +
| 4
 +
|-
|  
|  
 +
| 500mL
 +
| 8
 +
|-
|  
|  
-
|-
+
| 100mL
 +
| 8
 +
|-  
| Beakers
| Beakers
|  
|  
|  
|  
-
|-
+
|-
 +
|
 +
| 1L
 +
| 2
 +
|-
 +
|
 +
| 250mL
 +
| 8
 +
|-  
| Centrifuge tubes
| Centrifuge tubes
|  
|  
|  
|  
-
|-
+
|-
 +
|
 +
| 50mL
 +
| 100
 +
|-
 +
|
 +
| 15mL
 +
| 100
 +
|-  
| Graduated cylinders
| Graduated cylinders
|  
|  
|  
|  
-
|-
+
|-
 +
|
 +
|
 +
500mL
 +
| 2
 +
|-
 +
|
 +
| 100mL
 +
| 2
 +
|-  
| Electronic pipette tips (packages)
| Electronic pipette tips (packages)
|  
|  
|  
|  
 +
|-
 +
|
 +
| 1mL
 +
| 2
 +
|-
 +
|
 +
| 5mL
 +
| 2
 +
|-
|  
|  
 +
| 10mL
 +
| 2
 +
|-
|  
|  
 +
| 25mL
 +
| 2
 +
|-
 +
| pasteur pipettes
 +
|
 +
| 1 box
|-
|-
-
| Gloves (boxes)
+
| '''Gloves (boxes)'''
 +
|
 +
|
 +
|-
|  
|  
 +
| small
 +
| 1
 +
|-
|  
|  
 +
| medium
 +
| 4
 +
|-
|  
|  
 +
| large
 +
| 4
 +
|-
 +
| '''Plasticware'''
 +
|
 +
|
 +
|-
 +
| eppendorf tubes
|  
|  
-
|-
 
-
| Plasticware
 
|  
|  
-
|-
+
|-  
-
| centrifuge tubes
+
|  
|  
 +
| 1.5mL
 +
| 2000
 +
|-
|  
|  
-
|-
+
| 200μL (thin wall for PCR)
 +
| 1000
 +
|-  
| Pipette tips
| Pipette tips
|  
|  
|  
|  
 +
|-
|  
|  
-
|-
+
| 20μL
 +
| a lot
 +
|-
 +
|
 +
| 200μL
 +
| a lot
 +
|-
 +
|
 +
| 1000μL
 +
| a lot
 +
|-  
| Bacterial cell culture plates
| Bacterial cell culture plates
-
|-
+
|
 +
| 200
 +
|-  
| bacterial cell culture tubes
| bacterial cell culture tubes
 +
| (polypropylene)
 +
| 100
 +
|-
 +
| tube racks/holders
|  
|  
 +
|
 +
|-
 +
|
 +
| for 1.5mL tubes
 +
| 4
 +
|-
 +
|
 +
| for 200μL tubes
 +
| 2
 +
|-
 +
| '''Reagents'''
 +
|
 +
|
 +
|-
 +
| pH standard buffers for meter
 +
|
 +
| 1
|-
|-
-
| tube racks/holders
+
| 70% ethanol
|  
|  
|  
|  
 +
|-
 +
| anitibiotics
|  
|  
 +
|
 +
|-
 +
|
 +
| ampicillin
 +
|
 +
|-
 +
|
 +
| kanamycin
 +
|
 +
|-
 +
|
 +
| puromycin
 +
|
 +
|-
 +
| Agarose
 +
|
 +
|
 +
|-
 +
| Ethidium Bromide
 +
|
 +
|
 +
|-
 +
| tris[hydroxymethyl]aminomethane
 +
|
 +
|
 +
|-
 +
| Acetate
 +
|
 +
|
 +
|-
 +
| EDTA
 +
|
 +
|
 +
|-
 +
| Gel Loading Dye
 +
|
 +
|
 +
|-
 +
| DNA ladder
 +
|
 +
|
 +
|-
 +
| Restriction enzymes and Buffers
 +
|
 +
|
 +
|-
 +
|
 +
| EcoRI
 +
|
 +
|-
 +
|
 +
| SpeI
 +
|
 +
|-
 +
|
 +
| XbaI
 +
|
 +
|-
 +
|
 +
| PstI
 +
|
 +
|-
 +
| Taq Polymerase (Master Mix)
 +
|
 +
|
 +
|-
 +
| 100% ethanol
 +
|
 +
|
 +
|-
 +
| Socium Acetate
 +
|
 +
|
 +
|-
 +
| NaCl
 +
|
 +
|
 +
|-
 +
| T4 DNA ligase
 +
|
 +
|
 +
|-
 +
| T4 DNA ligase Buffer
 +
|
 +
|
 +
|-
 +
| liquid LB Broth
 +
|
 +
|
 +
|-
 +
| LB with agar
 +
|
 +
|
 +
|-
 +
| TSS Buffer
 +
|
 +
|
 +
|-
 +
| Mini-Prep Kit
 +
|
 +
|
 +
|-
 +
| Maxi-Prep Kit
 +
|
 +
|
 +
|-
|}
|}
-
 
-
      $ 400.00  Primers for PCR ($16 x 25bp) Invitrogen.com <http://invitrogen.com/> <http://invitrogen.com/>  custom primers 
 
-
      $ 800.00  Antibodies for identifying cell division proteins 4 x $200 
 
-
      $ 250.00  disposable lab supplies (pipette tips, ependorf tubes, falcon tubes, plates) 
 
-
      $ 600.00  Gel kit: Agarose gel aparatus, power source 
 
-
          TBE reagents to make cell buffer, Tris, Ethydium bromide, Agros 
 
-
          Taq polymerase kit, DNTP - Free bases (actg ready mixed) 
 
-
      $ 600.00  Restriction enzymes  $60 x 10 
 
-
      $ 300.00  Thermocycler 
 
-
      $ 500.00  misc. equipment and supplies 
 
-
      $ 4,000.00  DNA synthesis costs $1.40 per base. 
 
-
        Protein coding regions are about 700 to 1000 bases long  This budget is for 3 - 4 new proteins.
 

Latest revision as of 16:50, 19 June 2006

type/size quantity Have(x) Location Comments
Equipment
freezers/fridges
-80°C 1 we have space in a -80 JWW basement
-20°C 1 yes JWW228
4°C 1 yes JWW228
incubator (what kind?) 1 might work (heh) JWW228
pH meter 1
hot bath 1 we have access JWW218
cold bath 1
autoclave 1 we have access JWW 4th Floor
cell shaker inside 37 degree incubator 1 we have acess JWW 3rd floor
source of ddH2O 1
hot plates w/ stirring 2
stir bars (set) 1
PCR thermocycler 1
microcentrifuge (w/temp cntrl.) temp. cntrl not essential 1
centrifuge 50mL and 15mL 1 yes - 15 mL very old, JWW228
Electrophoresis gel set 4
Gel Power source 1
UV Gel viewer 1
UV Gel camera 1
Pipettemen
20μL 4
200μL 4
1000μL 4
Electronic pipettor can use 1-25mL pipettes 1
Manual pipettors / bulbs 2
bunsen burner 2
sparkers 2
wire loop to pick a bac clone 3
metal loop to spread bacteria 3
Glassware
Bottles
1L 4
500mL 8
100mL 8
Beakers
1L 2
250mL 8
Centrifuge tubes
50mL 100
15mL 100
Graduated cylinders

500mL

2
100mL 2
Electronic pipette tips (packages)
1mL 2
5mL 2
10mL 2
25mL 2
pasteur pipettes 1 box
Gloves (boxes)
small 1
medium 4
large 4
Plasticware
eppendorf tubes
1.5mL 2000
200μL (thin wall for PCR) 1000
Pipette tips
20μL a lot
200μL a lot
1000μL a lot
Bacterial cell culture plates 200
bacterial cell culture tubes (polypropylene) 100
tube racks/holders
for 1.5mL tubes 4
for 200μL tubes 2
Reagents
pH standard buffers for meter 1
70% ethanol
anitibiotics
ampicillin
kanamycin
puromycin
Agarose
Ethidium Bromide
tris[hydroxymethyl]aminomethane
Acetate
EDTA
Gel Loading Dye
DNA ladder
Restriction enzymes and Buffers
EcoRI
SpeI
XbaI
PstI
Taq Polymerase (Master Mix)
100% ethanol
Socium Acetate
NaCl
T4 DNA ligase
T4 DNA ligase Buffer
liquid LB Broth
LB with agar
TSS Buffer
Mini-Prep Kit
Maxi-Prep Kit
Retrieved from "http://2006.igem.org/wiki/index.php/Brown:Equipment_needed"
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