Davidson Assembly Plan
From 2006.igem.org
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[[Image:TT-REJPEG.jpg]] | [[Image:TT-REJPEG.jpg]] | ||
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| + | The Davidson cohort will also construct SuperParts necessary for the 1-pancake constructs in which the Kan resistance coding region is both forward and backward. | ||
| + | Note: Davidson will need to send the forward KanR to Western for use in their assembly. | ||
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| + | [[Image:SuperPartCJPEG.jpg]] | ||
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| + | [[Image:SuperPartD1JPEG.jpg]] | ||
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| + | [[Image:SuperPartD2JPEG.jpg]] | ||
Revision as of 01:11, 30 June 2006
Parts List
pLac promoter -- BBa_R0010 DNA-1 7K
pAra promoter -- BBa_I13453 DNA-2 13D
RBS -- BBa_B0030 DNA-1 3G
HixC – BBa_B?????
double forward terminator -- BBa_B0015 DNA-1 1I
double backward terminator – BBa_B0025 DNA-1 3E
KanR – BBa_B?????
Recombination enhancer -- BBa_J3101 (Davidson)
Hin -- BBa_J31000 (Davidson)
pAra promoter reverse – BBa_B????? (Western)
RBS reverse – BBa_B????? (Western)
Davidson will complete the TT-RE assembly below and send to Western.
The Davidson cohort will also construct SuperParts necessary for the 1-pancake constructs in which the Kan resistance coding region is both forward and backward. Note: Davidson will need to send the forward KanR to Western for use in their assembly.
