From 2006.igem.org

Revision as of 16:28, 12 July 2006 (view source) Fizzle6821 (Talk | contribs) (→RE and Hix) ← Older edit		Revision as of 18:37, 12 July 2006 (view source) Fizzle6821 (Talk | contribs) (→Cloning) Newer edit →
Line 7:		Line 7:
	*Tet-forward		*Tet-forward
	* Tet-backwards		* Tet-backwards
-	* <font color="red"> '''Cmr-backwards'''</font color>	+	* Cmr-backwards
-	** We are currently unsure if this is actually cloned due to the faint band of the insert. We are doing further studies of this to be sure that the insert is actually present. We have sent this to the Mammoths.	+	** The sample contains the insert but it is very faint as can be seen here[[Image:SNR_CB8_and Kan.jpg]]
	=RE and Hix=		=RE and Hix=

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Revision as of 18:37, 12 July 2006

Cloning

We have been able to successfully clone the following antibiotic pancakes

• Hin
• Hin with LVA degredation tag
• Cmr- forward
• Kan-forward
• Tet-forward
• Tet-backwards
• Cmr-backwards
  • The sample contains the insert but it is very faint as can be seen here

RE and Hix

We have been able to clone RE into cells. The RE that we are using has two point mutations but they should not compromise the activity of the Fis-binding sites please see the page for part Part BBa_J3101 [http://partsregistry.org/Part:BBa_J3101] for further details.

• As per the [http://2006.igem.org/Davidson_Assembly_Plan| assembly plan] we will be attaching the RE downstream of the double terminator BBa_B0015 [http://partsregistry.org/Part:BBa_B0015]

• We also have two cultures with the correct sequences for Hix C.
• We have sent a "Master Plate" with cultures of all of the things that we have cloned to the Mammoths.