The What's and How's
From 2006.igem.org
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* <u>MALCOLM</u> What is the protocol for assembly for the first stage (restriction digestions, ligations, transformations)? <br> | * <u>MALCOLM</u> What is the protocol for assembly for the first stage (restriction digestions, ligations, transformations)? <br> | ||
# [[http://www.bio.davidson.edu/courses/Molbio/Protocols/reagents.html Common molecular reagents]] | # [[http://www.bio.davidson.edu/courses/Molbio/Protocols/reagents.html Common molecular reagents]] | ||
| + | # [[http://www.bio.davidson.edu/courses/Molbio/Protocols/anneal_oligos.html Building dsDNA with Oligos]] | ||
# [[http://www.bio.davidson.edu/courses/Molbio/Protocols/pcr.html Setting up PCR mixtures]] | # [[http://www.bio.davidson.edu/courses/Molbio/Protocols/pcr.html Setting up PCR mixtures]] | ||
# [[http://www.bio.davidson.edu/courses/Molbio/Protocols/magnesium.html PCR and Mg<sup>2+</sup> concentration]] | # [[http://www.bio.davidson.edu/courses/Molbio/Protocols/magnesium.html PCR and Mg<sup>2+</sup> concentration]] | ||
Revision as of 18:58, 7 September 2006
- What is the list of DNA parts that we will need for the first stage, second stage, entire project?
- ERIN and SABRIYA Which DNA parts exist in the registry?Answer1More Parts
- ERIN and SABRIYAWhich DNA parts will have to be designed? Will they be synthesized or produced by PCR?
loxP sites Hin/Hix CRE gene Degradation Tags
- Which plasmids will be used from the registry?
- Where would biobricks be located?
- MALCOLM What is the protocol for assembly for the first stage (restriction digestions, ligations, transformations)?
- http://www.bio.davidson.edu/courses/Molbio/Protocols/reagents.html Common molecular reagents
- http://www.bio.davidson.edu/courses/Molbio/Protocols/anneal_oligos.html Building dsDNA with Oligos
- http://www.bio.davidson.edu/courses/Molbio/Protocols/pcr.html Setting up PCR mixtures
- [[http://www.bio.davidson.edu/courses/Molbio/Protocols/magnesium.html PCR and Mg2+ concentration]]
- http://www.bio.davidson.edu/courses/Molbio/Protocols/pourgel.html Pouring an agarose gel
- http://www.bio.davidson.edu/courses/Molbio/Protocols/molwt.html Calculate MWs
- http://www.bio.davidson.edu/courses/Molbio/Protocols/digestion.html Digest DNA with restriction enzymes
- http://www.bio.davidson.edu/courses/Molbio/Protocols/clean_short.html Clean DNA (short protocol)
- http://www.bio.davidson.edu/courses/Molbio/Protocols/gels2002/1kbladder.pdf 1kb MW markers
- http://www.bio.davidson.edu/courses/Molbio/Protocols/SAP.html Shrimp Alkaline Phosphatase
- http://www.bio.davidson.edu/courses/Molbio/Protocols/gelpure.html Gel Purification
- http://www.bio.davidson.edu/courses/Molbio/Protocols/ligation.html Ligation Protocol
- http://www.bio.davidson.edu/courses/Molbio/Protocols/Promegacompcells.pdf Transformation http://www.bio.davidson.edu/courses/Molbio/Protocols/transformation.html Short version
- http://www.bio.davidson.edu/courses/Molbio/Protocols/miniprepPrmega.html Promega miniprep
