Construction Intermediates

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'''pBad Promoter- RBS BBa_J3102''' [http://partsregistry.org/Part:BBa_J3102]
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Nicknames for the parts are in <font color='blue'>blue</font color>
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'''Recombinational Enhancer- Double Forward terminator'''- BBa_S03512 [http://partsregistry.org/Part:BBa_S03512]
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'''pBad Promoter- RBS''' BBa_J3102 [http://partsregistry.org/Part:BBa_J3102]
 +
* Used culture #3
 +
 
 +
'''pBad Promoter-Hix''' : BBa_S03529 [http://partsregistry.org/Part:BBa_S03529]
 +
*Used the Midi-prepped DNA that we recieved from the Mammoths
 +
 
 +
'''pLac Promoter-RBS''' : BBa_S03511 [http://partsregistry.org/Part:BBa_S03511]
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* Used culture #4
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 +
'''pBad Promotor-Hix-RBS''' <font color='blue'>(P-H-R)</font color> : BBa_S03530 [http://partsregistry.org/Part:BBa_S03530]
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* Used culture #3
 +
 
 +
'''Recombinational Enhancer- Double Forward terminator'''<font color='blue'>(RE-TT)</font color>: BBa_S03512 [http://partsregistry.org/Part:BBa_S03512]
* This part has a twin, S03513, that was made accidentally
* This part has a twin, S03513, that was made accidentally
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* This part was created on the registry by accident. It wsa never made in the laboratory.
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* Both parts were created on the registry by accident. It was never made in the laboratory.
 +
 
 +
'''Double Forward Terminator- Recombinational Enhancer'''<font color='blue'> (TT-RE) </font color>: BBa_S03516 [http://partsregistry.org/Part:BBa_S03516]
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*Used culture #1
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'''HinLVA-Double Forward Terminator'''<font color='blue'>(HLVA-TT)</font color>: BBa_S03514 [http://partsregistry.org/Part:BBa_S03514]
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* Used culture #1
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 +
'''Hin-Double Forward Terminator'''<font color='blue'>(H-TT)</font color>:  BBa_S03515 [http://partsregistry.org/Part:BBa_S03515]
 +
* Used culutre #3
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 +
'''Hix-Double Forward Terminator-RE'''<font color='blue'>(H-TT-RE)</font color> : BBa_S03522 [http://partsregistry.org/Part:BBa_S03522]
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*Used culture #1
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 +
'''TetR Forwards-HixC'''<font color='blue'>(TF-H)</font color>: BBa_S03523 [http://partsregistry.org/Part:BBa_S03523]
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* Used culutre #2
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'''Double Forward Terminator- Recombinational Enhancer''': BBa_S03516 [http://partsregistry.org/Part:BBa_S03516]
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'''TetR Forwards-Hix- Double Forward Terminator-RE'''<font color='blue'>(TF-H-TT-RE)</font color>: BBa_S03524 [http://partsregistry.org/Part:BBa_S03524]
 +
* Used culture #2
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'''HinLVA-Double Forward Terminator''': BBa_S03514 [http://partsregistry.org/Part:BBa_S03514]
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'''Hix-TetR Forwards-Hix-Double Forward Terminator-RE'''<font color='blue'>(H-TF-H-TT-RE) </font color>: BBa_S03527 [http://partsregistry.org/Part:BBa_S03527]
 +
* Used culture # 2
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'''Hin-Double Forward Terminator''': BBa_S03515 [http://partsregistry.org/Part:BBa_S03515]
+
'''TetR Backwards-Hix'''<font color='blue'>(TB-H)</font color> : BBa_S03525 [http://partsregistry.org/Part:BBa_S03525]
 +
* Used culture #4
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'''Hix-Double Forward Terminator-RE''' : BBa_S03522 [http://partsregistry.org/Part:BBa_S03522]
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'''TetR Backwards-Hix- Double Forward Terminator-RE'''<font color='blue'>(TB-H-TT-RE) </font color>: BBa_S03526 [http://partsregistry.org/Part:BBa_S03526]
 +
* Used culture # 3
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'''TetR Forwards-HixC''': BBa_S03523 [http://partsregistry.org/Part:BBa_S03523]
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'''Hix-TetR Backwards-Hix-Double Forward Terminator-RE'''<font color='blue'>(H-TB-H-TT-RE)</font color>: BBa_S03528 [http://partsregistry.org/Part:BBa_S03528]
 +
*Used culture #3
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'''TetR Forwards-Hix- Double Forward Terminator-RE''': BBa_S03524 [http://partsregistry.org/Part:BBa_S03524]
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'''TetR Backwards-RBS(backwards)'''<font color='blue'>(TB-RBS<sub>rev</sub>)</font color> : BBa_S03532 [http://partsregistry.org/Part:BBa_S03532]
 +
*Used culture #2
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'''Hix-TetR-Forwards-Hix-Double Forward Terminator-RE''': BBa_S03527 [http://partsregistry.org/Part:BBa_S03527]
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'''pBad-Hix C- TetR Backwards-RBS (backwards)'''<font color='blue'>(P-H-TB-RBS<sub>rev</sub>)</font color>  : BBa_S03533 [http://partsregistry.org/Part:BBa_S03533]
 +
*Used culture #1
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'''TetR Backwards-Hix''' : BBa_S03525 [http://partsregistry.org/Part:BBa_S03525]
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''' TetR Backwards- RBS Backwards-HixC- Double Forwards Terminator-RE'''<font color='blue'>(TB-RBS<sub>rev</sub>-H-TT-RE)</font color> BBa_S03534 [http://partsregistry.org/Part:BBa_S03534]
 +
* We chose not to use this part for ligation.
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'''TetR Backwards-Hix- Double Forward Terminator-RE''': BBa_S03526 [http://partsregistry.org/Part:BBa_S03526]
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'''Double Terminator Forwards-RBS-TetR Forwards'''<font color='blue'>(TT<sub>fwd</sub>-RBS-TF)</font color> BBa_S03595 [http://partsregistry.org/Part:BBa_S03595]
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* Constructed in plasmid pSB1AK3, then put into plasmid pSB1A2. Used culture #'s 1, 2 and 3 (combined)

Latest revision as of 21:41, 14 September 2006

Nicknames for the parts are in blue

pBad Promoter- RBS BBa_J3102 [http://partsregistry.org/Part:BBa_J3102]

  • Used culture #3

pBad Promoter-Hix : BBa_S03529 [http://partsregistry.org/Part:BBa_S03529]

  • Used the Midi-prepped DNA that we recieved from the Mammoths

pLac Promoter-RBS : BBa_S03511 [http://partsregistry.org/Part:BBa_S03511]

  • Used culture #4

pBad Promotor-Hix-RBS (P-H-R) : BBa_S03530 [http://partsregistry.org/Part:BBa_S03530]

  • Used culture #3

Recombinational Enhancer- Double Forward terminator(RE-TT): BBa_S03512 [http://partsregistry.org/Part:BBa_S03512]

  • This part has a twin, S03513, that was made accidentally
  • Both parts were created on the registry by accident. It was never made in the laboratory.

Double Forward Terminator- Recombinational Enhancer (TT-RE) : BBa_S03516 [http://partsregistry.org/Part:BBa_S03516]

  • Used culture #1

HinLVA-Double Forward Terminator(HLVA-TT): BBa_S03514 [http://partsregistry.org/Part:BBa_S03514]

  • Used culture #1

Hin-Double Forward Terminator(H-TT): BBa_S03515 [http://partsregistry.org/Part:BBa_S03515]

  • Used culutre #3

Hix-Double Forward Terminator-RE(H-TT-RE) : BBa_S03522 [http://partsregistry.org/Part:BBa_S03522]

  • Used culture #1

TetR Forwards-HixC(TF-H): BBa_S03523 [http://partsregistry.org/Part:BBa_S03523]

  • Used culutre #2

TetR Forwards-Hix- Double Forward Terminator-RE(TF-H-TT-RE): BBa_S03524 [http://partsregistry.org/Part:BBa_S03524]

  • Used culture #2

Hix-TetR Forwards-Hix-Double Forward Terminator-RE(H-TF-H-TT-RE) : BBa_S03527 [http://partsregistry.org/Part:BBa_S03527]

  • Used culture # 2

TetR Backwards-Hix(TB-H) : BBa_S03525 [http://partsregistry.org/Part:BBa_S03525]

  • Used culture #4

TetR Backwards-Hix- Double Forward Terminator-RE(TB-H-TT-RE) : BBa_S03526 [http://partsregistry.org/Part:BBa_S03526]

  • Used culture # 3

Hix-TetR Backwards-Hix-Double Forward Terminator-RE(H-TB-H-TT-RE): BBa_S03528 [http://partsregistry.org/Part:BBa_S03528]

  • Used culture #3

TetR Backwards-RBS(backwards)(TB-RBSrev) : BBa_S03532 [http://partsregistry.org/Part:BBa_S03532]

  • Used culture #2

pBad-Hix C- TetR Backwards-RBS (backwards)(P-H-TB-RBSrev)  : BBa_S03533 [http://partsregistry.org/Part:BBa_S03533]

  • Used culture #1

TetR Backwards- RBS Backwards-HixC- Double Forwards Terminator-RE(TB-RBSrev-H-TT-RE) BBa_S03534 [http://partsregistry.org/Part:BBa_S03534]

  • We chose not to use this part for ligation.

Double Terminator Forwards-RBS-TetR Forwards(TTfwd-RBS-TF) BBa_S03595 [http://partsregistry.org/Part:BBa_S03595]

  • Constructed in plasmid pSB1AK3, then put into plasmid pSB1A2. Used culture #'s 1, 2 and 3 (combined)
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