Lab Work

From 2006.igem.org

(Difference between revisions)
Jump to: navigation, search
(→10th July 2006)
 
(109 intermediate revisions not shown)
Line 1: Line 1:
-
===10th July 2006===
+
[[Image:encabezado3.jpg]]
-
The colonies transformed on the 6th made it this time, and we isolated the plasmid DNA from three individual colonies for each biobrick. We also transformed some E. coli with
+
-
{| border="1"
+
-
!width="40"|
+
-
!width="100"|
+
-
!width="100"|
+
-
!width="70"|
+
-
|-
+
-
| 23E || pSB1A3 || Plasmid || Plate 1 || AmpR
+
-
|}
+
-
To serve as an empty plasmid for the new LacZ and arsenic promoter/repressor parts which we will create.
+
-
===7th July 2006===
+
'''Lab Work'''
-
[[Image:DSCN0576.JPG|256px|thumb|left|Acid Production with LacZ and Lactose]]
+
-
When bacteria with the lacZ gene inserted are present in a medium containing lactose, the pH does drop significantly.
+
 +
[[Image:lab work 3.jpg]]
-
 
+
[[Lab work Schedule]]
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
===6th July 2006===
+
-
 
+
-
Unfortunately the colonies we plated on the 4th did not survive due to a problem with the competent cells we used, so today we repeated transforming and plating colonies containing the following parts:
+
-
 
+
-
{| border="1"
+
-
!width="40"|
+
-
!width="100"|
+
-
!width="100"|
+
-
!width="70"|
+
-
|-
+
-
| 9E || BBa_E0033 || LacZ alpha || Plate 2 || KanR
+
-
|-
+
-
| 1I || BBa_B0015 || Terminator || Plate 1 || AmpR
+
-
|-
+
-
| 7K || BBa_R0010 || IPTG responsive promoter || Plate 1 || AmpR
+
-
|-
+
-
| 3O || BBa_B0034 || RBS || Plate 1 || AmpR
+
-
|}
+
-
 
+
-
===4th July 2006===
+
-
 
+
-
We plated colonies containing plasmids with the following parts:
+
-
 
+
-
{| border="1"
+
-
!width="40"|
+
-
!width="100"|
+
-
!width="100"|
+
-
!width="70"|
+
-
|-
+
-
| 9E || BBa_E0033 || LacZ alpha || Plate 2 || KanR
+
-
|-
+
-
| 3P || BBa_0010 || Terminator || Plate 2 || AmpR
+
-
|-
+
-
| 7K || BBa_R0010 || IPTG responsive promoter || Plate 1 || AmpR
+
-
|}
+
-
 
+
-
 
+
-
[http://2006.igem.org/Standard_Protocols Standard Protocols]
+
-
 
+
-
 
+
-
[http://2006.igem.org/University_of_Edinburgh_2006 Main page]
+
-
__NOTOC__
+

Latest revision as of 04:17, 26 October 2006

Encabezado3.jpg

Lab Work

Lab work 3.jpg

Lab work Schedule

Retrieved from "http://2006.igem.org/wiki/index.php/Lab_Work"
Personal tools
Past/present/future years