Lab Work

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===11th July 2006===
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[[Image:encabezado3.jpg]]
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Today we tested the timed pH response in 2 cell cultures:  1 with the LacZ gene, which was activated by IPTG, and one that had this gene absent.
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'''Lab Work'''
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===10th July 2006===
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[[Image:lab work 3.jpg]]
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The colonies transformed on the 6th made it this time, and we isolated the plasmid DNA from three individual colonies for each biobrick. We also transformed some E. coli with
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| 23E || pSB1A3 || Plasmid || Plate 1 || AmpR
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to serve as an empty plasmid for the new LacZ and arsenic promoter/repressor parts which we will create.
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===7th July 2006===
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[[Lab work Schedule]]
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[[Image:DSCN0576.JPG|256px|thumb|left|Acid Production with LacZ and Lactose]]
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When bacteria with the lacZ gene inserted are present in a medium containing lactose, the pH does drop significantly.
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===6th July 2006===
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Unfortunately the colonies we plated on the 4th did not survive due to a problem with the competent cells we used, so today we repeated transforming and plating colonies containing the following parts:
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| 9E || BBa_E0033 || LacZ alpha || Plate 2 || KanR
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| 1I || BBa_B0015 || Terminator || Plate 1 || AmpR
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| 7K || BBa_R0010 || IPTG responsive promoter || Plate 1 || AmpR
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| 3O || BBa_B0034 || RBS || Plate 1 || AmpR
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===4th July 2006===
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We plated colonies containing plasmids with the following parts:
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|-
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| 9E || BBa_E0033 || LacZ alpha || Plate 2 || KanR
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|-
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| 3P || BBa_0010 || Terminator || Plate 2 || AmpR
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| 7K || BBa_R0010 || IPTG responsive promoter || Plate 1 || AmpR
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|}
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[http://2006.igem.org/Standard_Protocols Standard Protocols]
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[http://2006.igem.org/University_of_Edinburgh_2006 Main page]
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__NOTOC__
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Latest revision as of 04:17, 26 October 2006

Encabezado3.jpg

Lab Work

Lab work 3.jpg

Lab work Schedule

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