|
|
| Line 1: |
Line 1: |
| | {{Brown navigation bar}} | | {{Brown navigation bar}} |
| | | | |
| - | == Bacterial Freeze Tag ==
| |
| - | == [[Brown:2006/Magnetotactic Bacteria project]] ==
| |
| | | | |
| - | ==[[Overview]]== | + | {| cellspacing="2px" cellpadding="0" border="0" style="padding: 0; width: 750px; color: #000000; background-color: #ffffff;" |
| - | This project involves programming bacteria to be able to play a game of freeze tag. Bacteria will be engineered to swim around a microfluidics device until they reach a certain proximity to the 'IT' cell and then they will lose their ability to move. This loss of motility will be combined with a change in color from Green to Blue. When another bacterium, which is moving (not the 'IT' cell), reaches a certain proximity to the 'frozen' bacteria it will again regain its ability to move and turn from Blue to Yellow.
| + | | width=717px style="padding: 5px; background-color: #ffffff; border: 2px solid #993300;" | |
| | | | |
| - | ==[[Sender Cell]]==
| + | <h3>Intro </h3> |
| | + | intro |
| | | | |
| - | TetR promoted with LuxI downstream. LuxI is an enzyme that produces AHL and will produce the red fluorescent protein (RFP). The AHL produced is exported from the cell where it then forms a complex with the LuxR protein that is produced by the AHL sensor within the Receiver cell.
| + | {| cellspacing="2px" cellpadding="0" border="0" style="padding: 0px; width: 750px; color: #000000; background-color: #ffffff;" |
| | + | |-valign="top" |
| | + | |width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #993300;" | |
| | | | |
| - | [[Image:SenderCell.png|frame|AHL Sender Cell]]
| + | <h3>Freeze Tag</h3> |
| | | | |
| - | ==[[AHL Sensor]]== | + | *blah |
| | + | *blah |
| | + | |width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #993300;" | |
| | | | |
| - | The AHL sensor is TetR promoted and forms the LuxR protein which then forms a complex with AHL. This LuxR and AHL complex then activates the pLuxR promoter. Downstream of the pLuxR promoter is the LacI protein. LacI inhibits the pLac promoter on the "Freeze Machine".
| + | <h3>[[Brown:Tri-Stable toggle switch|Tri-Stable toggle switch]]</h3> |
| - | [[Image:AHLSensor.jpg|frame|AHL Sensor]] | + | |
| | | | |
| - | ==[['Freeze Machine']]== | + | |width=179.25px style="padding: 5px; background-color: #ffffff; border: 2px solid #993300;" | |
| - | A promoter that is regulated by LacI will promote the production of LasI, MotB, and cI. This will subsequently inhibit the production of CFP and LasR. In the presence of LacI, however, MotB, LasI, and cI will not be produced. CFP will therefore be produced along with LasR and LacI. This results in the "freezing" of the cell.
| + | |
| - | [[Image:FreezeMachine.png|frame|Freeze Machine]]
| + | |
| | | | |
| - | ==[["Dr. Unfreeze"]]==
| + | <h3>[[Brown:2006/Magnetotactic Bacteria project]] </h3> |
| - | "Dr. Unfreeze" is responsible for short circuiting the freeze machine. When it is activated by AI-1 and LasR it begins producing cI, which will inhibit pcI and allow the cell to begin producing MotB.
| + | <h3>Team logistics</h3> |
| - | [[Image:unfreeze.png|frame|Dr. Unfreeze]]
| + | |
| | + | *blah |
| | + | *Blah |
| | + | |
| | + | |
| | + | |
| | + | __NOTOC__ |
