Balloon Bacteria : Chiba 2006
From 2006.igem.org
(→Experiments) |
|||
Line 9: | Line 9: | ||
'''→World first bacteria that swells by lights.!!!''' | '''→World first bacteria that swells by lights.!!!''' | ||
+ | |||
+ | '''When growing...''' | ||
+ | |||
+ | The movement of the E. coli can be obvered with the naked eyes! | ||
+ | |||
+ | "Big" E. coli is easily sorted by FACS! | ||
+ | |||
+ | Make much more proteins/cell! | ||
+ | |||
Line 32: | Line 41: | ||
[[Image:design3.jpg]] | [[Image:design3.jpg]] | ||
+ | |||
Line 38: | Line 48: | ||
#We made inducible rodA''mut''. | #We made inducible rodA''mut''. | ||
- | #We made CI inverter and | + | #We made CI inverter and compared it. |
+ | |||
+ | |||
== Results == | == Results == |
Revision as of 10:00, 30 October 2006
Contents |
Concept
The light in the morning leaking from a gap between the curtains... this morning lights calls and awakes the E. coli.
Suprise!!
The E. coli become bigger and bigger like BALLOON!!!
→World first bacteria that swells by lights.!!!
When growing...
The movement of the E. coli can be obvered with the naked eyes!
"Big" E. coli is easily sorted by FACS!
Make much more proteins/cell!
Design
First
The E. coli doesn't have photoreceptor. But...
We have Cph8(utwxas/uCSF;iGEM2005)in our hand
Second
The E. coli divides the cells by forming "partition". So...
'Expression of the enzyme in septem formation is knocked-down.
Third
E. coli rather "extend" due to the action of RodA. So...
We make inducible RodAmut.
Experiments
- We made inducible rodAmut.
- We made CI inverter and compared it.
Results
Member
Chiba 2006 http://chem.tf.chiba-u.jp/igem/ iGEM Chiba
『je』team