Balloon Bacteria : Chiba 2006
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[[Image:rodAmut.jpg]] | [[Image:rodAmut.jpg]] | ||
| - | ...show more.[[ | + | ...show more.[[rodA''mut'']] |
'''2.CI inverter''' | '''2.CI inverter''' | ||
Revision as of 10:26, 30 October 2006
Contents |
Concept
The light in the morning leaking from a gap between the curtains... this morning lights calls and awakes the E. coli.
Suprise!!
The E. coli become bigger and bigger like BALLOON!!!
→World first bacteria that swells by lights.!!!
When growing...
The movement of the E. coli can be obvered with the naked eyes !
"Big" E. coli is easily sorted by FACS !
Make much more proteins/cell !
Design
First
The E. coli doesn't have photoreceptor. But...
We have Cph8(utwxas/uCSF;iGEM2005)in our hand
Second
The E. coli divides the cells by forming "partition". So...
'Expression of the enzyme in septem formation is knocked-down.
Third
E. coli rather "extend" due to the action of RodA. So...
We make inducible RodAmut.
Experiments
- We made inducible rodAmut.
- We made CI inverter and compared it.
Results
1.rodAmut
...show more.[[rodAmut]]
2.CI inverter
...show more.CI inverter
Member
Chiba 2006 http://chem.tf.chiba-u.jp/igem/ iGEM Chiba
『je』team
