Synchronization of Cell Cycles
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==Synchronization of Cell Cycles== | ==Synchronization of Cell Cycles== | ||
| - | + | ==Group II== | |
| - | We are interested in synthetically synchronizing cell cycles. In our genetic construct, we have used Pcya (adenylate cyclase promoter-that is known to oscillate in cell cycle dependant manner) as a core oscillator.LuxI tagged with cfp(+LVA) is expressed from this promoter.LuxI oscillations will result in AI(autoinducer) oscillations and this would result in oscillations of AI dependant promoter. YFP is expressed from such a promoter. | + | ===Aim=== |
| + | |||
| + | We are interested in synthetically synchronizing cell cycles. | ||
| + | |||
| + | ===Approach=== | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | ===Constructs=== | ||
| + | In our genetic construct, we have used Pcya (adenylate cyclase promoter-that is known to oscillate in cell cycle dependant manner) as a core oscillator. | ||
| + | |||
| + | |||
| + | ===Testing oscillations=== | ||
| + | LuxI tagged with cfp(+LVA) is expressed from this promoter.LuxI oscillations will result in AI(autoinducer) oscillations and this would result in oscillations of AI dependant promoter. YFP is expressed from such a promoter. | ||
CFP expression is readout for the core oscillator-Pcya and YFP is readout of the expression of the quorum sensing promoter-Plux. | CFP expression is readout for the core oscillator-Pcya and YFP is readout of the expression of the quorum sensing promoter-Plux. | ||
Revision as of 11:10, 30 October 2006
Contents |
Synchronization of Cell Cycles
Group II
Aim
We are interested in synthetically synchronizing cell cycles.
Approach
Constructs
In our genetic construct, we have used Pcya (adenylate cyclase promoter-that is known to oscillate in cell cycle dependant manner) as a core oscillator.
Testing oscillations
LuxI tagged with cfp(+LVA) is expressed from this promoter.LuxI oscillations will result in AI(autoinducer) oscillations and this would result in oscillations of AI dependant promoter. YFP is expressed from such a promoter. CFP expression is readout for the core oscillator-Pcya and YFP is readout of the expression of the quorum sensing promoter-Plux.
Y axis is the mean cfp fluorescence of a cell. X-axis is a correlate of cell cycle phase.Here, we have used cell length as a crude correlate for cell cycle phase.The same data is platted twice for visual representation of oscillation. RFP expression is control.
Y axis is the mean yfp fluorescence of a cell. X-axis is major axis length of a cell which is a correlate of cell cycle phase.
--Sugat 06:49, 8 July 2006 (EDT)
