McGill University 2006

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<h3><ul><li>''' Team 2: Repressilator'''</ul></h3>
<h3><ul><li>''' Team 2: Repressilator'''</ul></h3>
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Our project concerns the development of a repressilator coupled to quorum sensing as described by Jordi Garcia-Ojalvo, Michael B. Elowitz and Steven H. Strogatz in PNAS. The Elowitz Repressilator attempts to decrease the loss of standard oscillations that previous repressilators faced by utilizing quorum sensing as a means of synchronizing and maintaining standard oscillations. We expanded on this theory by adding YFP and CFP to allow a visual confirmation of the oscillation, and a TetR promoter in front of the LuxR gene and cI after the LuxI gene.  Our hopes were that this would assist in standardizing the oscillation of the bacteria.
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Our project is based on the repressilator system coupled to quorum sensing as described by Jordi Garcia-Ojalvo, Michael B. Elowitz and Steven H. Strogatz in "Modeling a synthetic multicellular clock: Repressilators coupled by quorum sensing" (PNAS). We attempt to visualize the synchronization of the oscillatory phase between cells by the addition of the CFP reporter gene. We expand on this theory by placing cI under the control of pTet, hoping that this would assist in synchronizing the oscillations.
[[Theory Behind the Oscillator|Theory Behind the Oscillator]]
[[Theory Behind the Oscillator|Theory Behind the Oscillator]]

Revision as of 19:20, 30 October 2006

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Welcome to the McGill Wiki! Basically, we're a team of 12 undergrads, 1 grad student, and 1 professor who like to have fun and clone things in our spare time. McGill University is located in Montreal, Quebec, which has given us wonderful opportunities to balance lab work with festivals and general craziness. iGEM has been a great opportunity to get lab experience while having the freedom to be creative, and we look forward to meeting the other teams at the jamboree!

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Projects

  • Team 1: Split YFP

This project on fluorescence complementation involves the joining of two proteins, Jun and Fos, each fused to a half terminus of YFP. Both of these chimeric proteins were fused to a beta gene that codes for a membrane protein. Then, two cell populations - one expressing Jun-beta-YFPN and the other Fos-beta-YFPC - were combined, ideally resulting in the fusion of the Jun and Fos proteins on the cell membrane. The two halves of the YFP protein would bind as well, giving rise to fluorescence.

Background

Methods and Materials

Results

  • Team 2: Repressilator

Our project is based on the repressilator system coupled to quorum sensing as described by Jordi Garcia-Ojalvo, Michael B. Elowitz and Steven H. Strogatz in "Modeling a synthetic multicellular clock: Repressilators coupled by quorum sensing" (PNAS). We attempt to visualize the synchronization of the oscillatory phase between cells by the addition of the CFP reporter gene. We expand on this theory by placing cI under the control of pTet, hoping that this would assist in synchronizing the oscillations.

Theory Behind the Oscillator

Methods and Materials

Results

Lab Procedures

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Club

Just for Fun

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