Freezer Stocks
From 2006.igem.org
(Difference between revisions)
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'''GROUP (CAP COLOR)'''<br> | '''GROUP (CAP COLOR)'''<br> | ||
- | '''CONSTRUCT''' -- '''PART NO.''' -- '''SOURCE COLONY/ PLATE''' -- '''DATE FROZEN'''<br><br> | + | '''CONSTRUCT''' -- '''VECTOR''' -- '''PART NO.''' -- '''SOURCE COLONY/ PLATE''' -- '''DATE FROZEN'''<br><br> |
'''Basic Parts''' (<font style="background-color: lightgreen">green</font>) | '''Basic Parts''' (<font style="background-color: lightgreen">green</font>) | ||
- | + | *pSB1A7 insulated cloning vector -- N/A -- <partinfo>pSB1A7</partinfo> -- IGEM 2006 Dav. Amp Vectors -- 1/29/07 | |
- | *pSB1A7 insulated cloning vector -- <partinfo>pSB1A7</partinfo> -- IGEM 2006 Dav. Amp Vectors -- 1/29/07 | + | *RFP reverse -- 1A3 -- <partinfo>BBa_J31008</partinfo> -- streak clone #1 (KH 020407) -- 2/19/07 |
- | *RFP reverse -- <partinfo>BBa_J31008</partinfo> -- | + | *Hin-100 -- 1A2 -- <partinfo>BBa_J31000</partinfo> -- 6 (EZ Basic 080306) -- 8/17/06 |
- | *Hin-100 -- <partinfo>BBa_J31000</partinfo> -- 6 (EZ Basic 080306) -- 8/17/06 | + | *Hin LVA -- 1A2 -- <partinfo>BBa_J31001</partinfo> -- 10 (EZ Basic 080306) -- 8/17/06 |
- | *Hin LVA -- <partinfo>BBa_J31001</partinfo> -- 10 (EZ Basic 080306) -- 8/17/06 | + | *RE -- 1A2 -- <partinfo>BBa_J3101</partinfo> -- 3 (EZ Basic 080306) -- 8/17/06 |
- | *RE -- <partinfo>BBa_J3101</partinfo> -- 3 (EZ Basic 080306) -- 8/17/06 | + | *Hix C -- 1A2 -- <partinfo>BBa_J44000</partinfo> -- 4 (EZ Basic 080306) -- 8/17/06 |
- | *Hix C -- <partinfo>BBa_J44000</partinfo> -- 4 (EZ Basic 080306) -- 8/17/06 | + | *pLac promoter -- 1A2 -- <partinfo>BBa_R0010</partinfo> -- SR plate 071206 -- 8/17/06 |
- | *pLac promoter -- <partinfo>BBa_R0010</partinfo> -- SR plate 071206 -- 8/17/06 | + | *pBad promoter -- 1A3 -- <partinfo>BBa_I13453</partinfo> -- SR plate 071206 -- 8/17/06 |
- | *pBad promoter -- <partinfo>BBa_I13453</partinfo> -- SR plate 071206 -- 8/17/06 | + | *RBS forward -- 1A2 -- <partinfo>BBa_B0030</partinfo> -- SR plate 071206 -- 8/17/06 |
- | *RBS forward -- <partinfo>BBa_B0030</partinfo> -- SR plate 071206 -- 8/17/06 | + | *RBS backward -- 1A2 -- <partinfo>BBa_J44001</partinfo> -- Eckdahl streak -- 8/17/06 |
- | *RBS backward -- <partinfo>BBa_J44001</partinfo> -- Eckdahl streak -- 8/17/06 | + | *TT forward -- 1AK3 -- <partinfo>BBa_B0015</partinfo> -- AMC streak 071806 -- 8/17/06 |
- | *TT forward -- <partinfo>BBa_B0015</partinfo> -- AMC streak 071806 -- 8/17/06 | + | *Tet forward -- 1A2 -- <partinfo>BBa_J31007</partinfo> -- 11 (EZ Basic 080306) -- 8/17/06 |
- | *Tet forward -- <partinfo>BBa_J31007</partinfo> -- 11 (EZ Basic 080306) -- 8/17/06 | + | *Tet backward -- 1A2 -- <partinfo>BBa_J31006</partinfo> -- 12 (EZ Basic 080306) -- 8/17/06 |
- | *Tet backward -- <partinfo>BBa_J31006</partinfo> -- 12 (EZ Basic 080306) -- 8/17/06 | + | *CmR forward -- 1A2 -- <partinfo>BBa_J31005</partinfo> -- 13 (EZ Basic 080306) -- 8/17/06 |
- | *CmR forward -- <partinfo>BBa_J31005</partinfo> -- 13 (EZ Basic 080306) -- 8/17/06 | + | *CmR backward -- 1A2 -- <partinfo>BBa_J31004</partinfo> -- 15 (EZ Basic 080306) -- 8/17/06 |
- | *CmR backward -- <partinfo>BBa_J31004</partinfo> -- 15 (EZ Basic 080306) -- 8/17/06 | + | *Kan forward -- 1A2 -- <partinfo>BBa_J31003</partinfo> -- 14 (EZ Basic 080306) -- 8/17/06 |
- | *Kan forward -- <partinfo>BBa_J31003</partinfo> -- 14 (EZ Basic 080306) -- 8/17/06 | + | *Kan backward -- ? -- <partinfo>BBa_J31002</partinfo> -- IGEM 2006 Dav. Amp Vectors -- 1/29/07 |
---- | ---- | ||
'''Intermediates''' (<font style="background-color: yellow">yellow</font>) | '''Intermediates''' (<font style="background-color: yellow">yellow</font>) | ||
- | *RFPrev-RBSrev -- <partinfo>BBa_S03644</partinfo> -- | + | *RFPrev-RBSrev -- <partinfo>BBa_S03644</partinfo> -- streak (KH 021507) -- 2/19/07 |
*RFPrev-RBSrev -- <partinfo>BBa_J31011</partinfo> -- IGEM 2006 Dav. Amp Vectors spot 3 -- 1/29/07 | *RFPrev-RBSrev -- <partinfo>BBa_J31011</partinfo> -- IGEM 2006 Dav. Amp Vectors spot 3 -- 1/29/07 | ||
*"AraC-PC, Hin" -- <partinfo>BBa_J3107</partinfo> -- IGEM 2006 Dav. Kan Vectors spot 4 -- 1/29/07 | *"AraC-PC, Hin" -- <partinfo>BBa_J3107</partinfo> -- IGEM 2006 Dav. Kan Vectors spot 4 -- 1/29/07 | ||
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*TB-Hix-TT-RE -- <partinfo>BBa_S03526</partinfo> -- 12 (SR 080106) -- 8/17/06 | *TB-Hix-TT-RE -- <partinfo>BBa_S03526</partinfo> -- 12 (SR 080106) -- 8/17/06 | ||
*Hix-TB-Hix-RE -- <partinfo>BBa_S03528</partinfo> -- 13 (SR 080106) -- 8/17/06 | *Hix-TB-Hix-RE -- <partinfo>BBa_S03528</partinfo> -- 13 (SR 080106) -- 8/17/06 | ||
- | *TB-RBS -- <partinfo>BBa_S03532</partinfo> -- 15 (SR 080106) -- 8/17/06 | + | *TB-RBS -- ? -- <partinfo>BBa_S03532</partinfo> -- 15 (SR 080106) -- 8/17/06 |
- | *pBad-Hix-TB-RBS -- <partinfo>BBa_S03533</partinfo> -- 16 (SR 080106) -- 8/17/0 | + | *pBad-Hix-TB-RBS -- ? -- <partinfo>BBa_S03533</partinfo> -- 16 (SR 080106) -- 8/17/0 |
- | *TB-RBS-Hix-TT-RE -- <partinfo>BBa_S03534</partinfo> -- 1 (EZ tet+ 080306) -- 8/17/06 | + | *TB-RBS-Hix-TT-RE -- ? -- <partinfo>BBa_S03534</partinfo> -- 1 (EZ tet+ 080306) -- 8/17/06 |
- | *pLac-Hix -- <partinfo>BBa_S03645</partinfo>-- | + | *pLac-Hix -- 1A2 -- <partinfo>BBa_S03645</partinfo>-- clone #1 (KH 021707) -- 3/19/07 |
*RFPrev-RBSrev-hix -- <partinfo>BBa_S03648</partinfo>-- RFPrevRBSrev-hix (1A2) clone #1 K.H. 2/17/07 -- 3/19/07 | *RFPrev-RBSrev-hix -- <partinfo>BBa_S03648</partinfo>-- RFPrevRBSrev-hix (1A2) clone #1 K.H. 2/17/07 -- 3/19/07 | ||
*RFPrev-RBSrev-hix-RBS-TetF -- <partinfo>BBa_S03649</partinfo> -- RFPrevRBSrev-hix-pLac-hix-RBS-TetF in pSB1a& (#2) K.H. 2/28/07-- 3/19/07 | *RFPrev-RBSrev-hix-RBS-TetF -- <partinfo>BBa_S03649</partinfo> -- RFPrevRBSrev-hix-pLac-hix-RBS-TetF in pSB1a& (#2) K.H. 2/28/07-- 3/19/07 |
Revision as of 00:00, 8 May 2007
GROUP (CAP COLOR)
CONSTRUCT -- VECTOR -- PART NO. -- SOURCE COLONY/ PLATE -- DATE FROZEN
Basic Parts (green)
- pSB1A7 insulated cloning vector -- N/A -- -- IGEM 2006 Dav. Amp Vectors -- 1/29/07
- RFP reverse -- 1A3 -- -- streak clone #1 (KH 020407) -- 2/19/07
- Hin-100 -- 1A2 -- -- 6 (EZ Basic 080306) -- 8/17/06
- Hin LVA -- 1A2 -- -- 10 (EZ Basic 080306) -- 8/17/06
- RE -- 1A2 -- -- 3 (EZ Basic 080306) -- 8/17/06
- Hix C -- 1A2 -- -- 4 (EZ Basic 080306) -- 8/17/06
- pLac promoter -- 1A2 -- -- SR plate 071206 -- 8/17/06
- pBad promoter -- 1A3 -- -- SR plate 071206 -- 8/17/06
- RBS forward -- 1A2 -- -- SR plate 071206 -- 8/17/06
- RBS backward -- 1A2 -- -- Eckdahl streak -- 8/17/06
- TT forward -- 1AK3 -- -- AMC streak 071806 -- 8/17/06
- Tet forward -- 1A2 -- -- 11 (EZ Basic 080306) -- 8/17/06
- Tet backward -- 1A2 -- -- 12 (EZ Basic 080306) -- 8/17/06
- CmR forward -- 1A2 -- -- 13 (EZ Basic 080306) -- 8/17/06
- CmR backward -- 1A2 -- -- 15 (EZ Basic 080306) -- 8/17/06
- Kan forward -- 1A2 -- -- 14 (EZ Basic 080306) -- 8/17/06
- Kan backward -- ? -- -- IGEM 2006 Dav. Amp Vectors -- 1/29/07
Intermediates (yellow)
- RFPrev-RBSrev -- -- streak (KH 021507) -- 2/19/07
- RFPrev-RBSrev -- -- IGEM 2006 Dav. Amp Vectors spot 3 -- 1/29/07
- "AraC-PC, Hin" -- -- IGEM 2006 Dav. Kan Vectors spot 4 -- 1/29/07
- "AraC-PC, HinLVA" -- -- IGEM 2006 Dav. Kan Vectors spot 5 -- 1/29/07
- AraCPCrev-TT -- -- IGEM 2006 Dav. Kan Vectors spot 6 -- 1/29/07
- TT-RBS-TetF -- -- IGEM 2006 Dav. Amp Vectors spot 7 -- 1/29/07
- KanB-RBSrev -- -- IGEM 2006 Dav. Amp Vectors spot 8 -- 1/29/07
- RBS-TF-HixC-TT-RE -- -- IGEM 2006 Dav. Amp Vectors spot 9 -- 1/29/07
- TB-RBSrev-hix -- -- IGEM 2006 Dav. Amp Vectors spot 11 -- 1/29/07
- Hix-pBadrev-Hix -- -- IGEM 2006 Dav. Amp Vectors spot 12 -- 1/29/07
- Hix-pBadrev-Hix-RBS-TF -- -- IGEM 2006 Dav. Amp Vectors spot 13 -- 1/29/07
- HixC-TetB-RBSrev-HixC -- -- KH 12/11/06 -- 1/29/07
- pBad-RBS -- -- 1 (SR 080106) -- 8/17/06
- pBad-Hix -- -- Eckdahl streak -- 8/17/06
- pLac-RBS -- -- 14 (SR 080106) -- 8/17/06
- pBad-Hix-RBS -- -- 3 (SR 080106) -- 8/17/06
- TT-RE -- -- 4 (SR 080106) -- 8/17/06
- HinLVA-TT -- -- 5 (SR 080106) --8/17/06
- Hin-TT -- -- 6 (SR 080106) -- 8/17/06
- Hix-TT-RE -- -- 7 (SR 080106) -- 8/17/06
- TF-Hix -- -- 8 (SR 080106) -- 8/17/06
- TF-Hix-TT-RE -- -- 9 (SR 080106) -- 8/17/06
- Hix-TF-Hix-TT-RE -- -- 10 (SR 080106) -- 8/17/06
- TB-Hix -- -- 11 (SR 080106) -- 8/17/06
- TB-Hix-TT-RE -- -- 12 (SR 080106) -- 8/17/06
- Hix-TB-Hix-RE -- -- 13 (SR 080106) -- 8/17/06
- TB-RBS -- ? -- -- 15 (SR 080106) -- 8/17/06
- pBad-Hix-TB-RBS -- ? -- -- 16 (SR 080106) -- 8/17/0
- TB-RBS-Hix-TT-RE -- ? -- -- 1 (EZ tet+ 080306) -- 8/17/06
- pLac-Hix -- 1A2 -- -- clone #1 (KH 021707) -- 3/19/07
- RFPrev-RBSrev-hix -- -- RFPrevRBSrev-hix (1A2) clone #1 K.H. 2/17/07 -- 3/19/07
- RFPrev-RBSrev-hix-RBS-TetF -- -- RFPrevRBSrev-hix-pLac-hix-RBS-TetF in pSB1a& (#2) K.H. 2/28/07-- 3/19/07
- pLac-Hix-RBS-TF -- -- pLac-hix-RBS-TetF in 1A7 K.H. 2/21/07 -- 3/19/07
- RBS-TetF -- N/A -- RBS-TetF in pSB1A7 K.H. 3/15/07 -- 3/19/07
Pancake Stacks (red)
- pBad-Hix-RBS-TF-Hix-TT-RE -- -- 6 (EZ tet+ 080306)-- 8/17/06
- pBad-Hix-TB-RBS-Hix-TT-RE -- -- 2 (EZ tet+ 080306)-- 8/17/06
- pBad-RBS-Hix-TF-Hix-TT-RE -- -- 8 (EZ tet- 080306)-- 8/17/06
- TT-(1,2) in 1A3 -- -- N/A --2/19/07
- TT-(1,2) in 3K3 -- -- N/A -- 2/21/07
- TT-(1,2) in 4A3 -- N/A -- N/A -- not yet made
- HixC-pBad-HixC-RBS-TetF-HixC-TT-RE -- -- IGEM 2006 Dav. Amp Vectors spot 10 -- 1/29/07
- HixC-pBadrev-HixC-TB-RBSrev-HixC -- -- IGEM 2006 Dav. Amp Vectors spot 14 -- 1/29/07
- (-2,-1) HixC-TetB-RBSrev-HixC-pBadrev-HixC -- -- KH 12/14/06 or KH 12/16/06 depending on vector -- 1/29/07