Freezer Stocks
From 2006.igem.org
(Difference between revisions)
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*CmR backward -- 1A2 -- <partinfo>BBa_J31004</partinfo> -- 15 (EZ Basic 080306) -- 8/17/06 | *CmR backward -- 1A2 -- <partinfo>BBa_J31004</partinfo> -- 15 (EZ Basic 080306) -- 8/17/06 | ||
*Kan forward -- 1A2 -- <partinfo>BBa_J31003</partinfo> -- 14 (EZ Basic 080306) -- 8/17/06 | *Kan forward -- 1A2 -- <partinfo>BBa_J31003</partinfo> -- 14 (EZ Basic 080306) -- 8/17/06 | ||
- | *Kan backward -- ? -- <partinfo>BBa_J31002</partinfo> -- IGEM 2006 Dav. Amp Vectors -- 1/29/07 | + | *Kan backward -- 1A2? -- <partinfo>BBa_J31002</partinfo> -- IGEM 2006 Dav. Amp Vectors -- 1/29/07 |
+ | *pLac<sub>rev</sub> -- 1A3 -- <partinfo>BBa_J31013</partinfo> -- 1 (KH 040207) -- 5/08/07 | ||
---- | ---- | ||
'''Intermediates''' (<font style="background-color: yellow">yellow</font>) | '''Intermediates''' (<font style="background-color: yellow">yellow</font>) | ||
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*TT-(1,2) -- 3K3 -- <partinfo>BBa_J31012</partinfo> -- N/A -- 2/21/07 | *TT-(1,2) -- 3K3 -- <partinfo>BBa_J31012</partinfo> -- N/A -- 2/21/07 | ||
*TT-(1,2) -- 4A3 -- N/A -- N/A -- not yet made | *TT-(1,2) -- 4A3 -- N/A -- N/A -- not yet made | ||
- | *(1,2) HixC-pBad-HixC-RBS-TetF-HixC-TT-RE -- <partinfo>BBa_S03635</partinfo> -- IGEM 2006 Dav. Amp Vectors spot 10 -- 1/29/07 | + | *(1,2) HixC-pBad-HixC-RBS-TetF-HixC-TT-RE -- 1A2 -- <partinfo>BBa_S03635</partinfo> -- IGEM 2006 Dav. Amp Vectors spot 10 -- 1/29/07 |
- | *(-1,-2) HixC-pBadrev-HixC-TB-RBSrev-HixC -- <partinfo>BBa_S03639</partinfo> -- IGEM 2006 Dav. Amp Vectors spot 14 -- 1/29/07 | + | *(-1,-2) HixC-pBadrev-HixC-TB-RBSrev-HixC -- 1A2 -- <partinfo>BBa_S03639</partinfo> -- IGEM 2006 Dav. Amp Vectors spot 14 -- 1/29/07 |
- | *(-2,-1) HixC-TetB-RBSrev-HixC-pBadrev-HixC -- <partinfo>BBa_S03642</partinfo> -- KH 12/14/06 or KH 12/16/06 depending on vector -- 1/29/07 | + | *(-2,-1) HixC-TetB-RBSrev-HixC-pBadrev-HixC -- 1A2-- <partinfo>BBa_S03642</partinfo> -- KH 12/14/06 or KH 12/16/06 depending on vector -- 1/29/07 |
+ | * (1)2 RFP<sub>rev</sub>-RBS<sub>rev</sub>-HixC-pLac-HixC-RBS-TetF -- 1A7 -- <partinfo>BBa_S03649</partinfo> -- (KH 022807) -- 5/08/07 | ||
+ | * (1)-2 RFP<sub>rev</sub>-RBS<sub>rev</sub>-HixC-pLac-HixC-TetB-RBS<sub>rev</sub> -- 1A7 -- <partinfo>BBa_S03673</partinfo> -- 2 (KH 032507) -- 5/08/07 |
Revision as of 01:59, 17 May 2007
GROUP (CAP COLOR)
CONSTRUCT -- VECTOR -- PART NO. -- SOURCE COLONY/ PLATE -- DATE FROZEN
Basic Parts (green)
- pSB1A7 insulated cloning vector -- N/A -- -- IGEM 2006 Dav. Amp Vectors -- 1/29/07
- RFP reverse -- 1A3 -- -- streak clone #1 (KH 020407) -- 2/19/07
- Hin-100 -- 1A2 -- -- 6 (EZ Basic 080306) -- 8/17/06
- Hin LVA -- 1A2 -- -- 10 (EZ Basic 080306) -- 8/17/06
- RE -- 1A2 -- -- 3 (EZ Basic 080306) -- 8/17/06
- Hix C -- 1A2 -- -- 4 (EZ Basic 080306) -- 8/17/06
- pLac promoter -- 1A2 -- -- SR plate 071206 -- 8/17/06
- pBad promoter -- 1A3 -- -- SR plate 071206 -- 8/17/06
- RBS forward -- 1A2 -- -- SR plate 071206 -- 8/17/06
- RBS backward -- 1A2 -- -- Eckdahl streak -- 8/17/06
- TT forward -- 1AK3 -- -- AMC streak 071806 -- 8/17/06
- Tet forward -- 1A2 -- -- 11 (EZ Basic 080306) -- 8/17/06
- Tet backward -- 1A2 -- -- 12 (EZ Basic 080306) -- 8/17/06
- CmR forward -- 1A2 -- -- 13 (EZ Basic 080306) -- 8/17/06
- CmR backward -- 1A2 -- -- 15 (EZ Basic 080306) -- 8/17/06
- Kan forward -- 1A2 -- -- 14 (EZ Basic 080306) -- 8/17/06
- Kan backward -- 1A2? -- -- IGEM 2006 Dav. Amp Vectors -- 1/29/07
- pLacrev -- 1A3 -- -- 1 (KH 040207) -- 5/08/07
Intermediates (yellow)
- RFPrev-RBSrev -- 1A2 -- -- streak (KH 021507) -- 2/19/07 (used in subsequent assemblies)
- RFPrev-RBSrev -- 1A2 -- -- IGEM 2006 Dav. Amp Vectors spot 3 -- 1/29/07 (not used in subsequent assemblies)
- "AraC-PC, Hin" -- ? -- -- IGEM 2006 Dav. Kan Vectors spot 4 -- 1/29/07
- "AraC-PC, HinLVA" -- ? -- -- IGEM 2006 Dav. Kan Vectors spot 5 -- 1/29/07
- AraCPCrev-TT -- ? -- -- IGEM 2006 Dav. Kan Vectors spot 6 -- 1/29/07
- TT-RBS-TetF -- ? -- -- IGEM 2006 Dav. Amp Vectors spot 7 -- 1/29/07
- KanB-RBSrev -- 1A3 -- -- IGEM 2006 Dav. Amp Vectors spot 8 -- 1/29/07
- RBS-TF-HixC-TT-RE -- -- IGEM 2006 Dav. Amp Vectors spot 9 -- 1/29/07
- TB-RBSrev-hix -- -- IGEM 2006 Dav. Amp Vectors spot 11 -- 1/29/07
- Hix-pBadrev-Hix -- -- IGEM 2006 Dav. Amp Vectors spot 12 -- 1/29/07
- Hix-pBadrev-Hix-RBS-TF -- ? -- -- IGEM 2006 Dav. Amp Vectors spot 13 -- 1/29/07
- Hix-TetB-RBSrev-Hix -- ? -- -- (KH 121106) -- 1/29/07
- pBad-RBS -- 1A2 -- -- 1 (SR 080106) -- 8/17/06
- pBad-Hix -- 1A2 -- -- Eckdahl streak -- 8/17/06
- pLac-RBS -- 1A2 -- -- 14 (SR 080106) -- 8/17/06
- pBad-Hix-RBS -- 1A2 -- -- 3 (SR 080106) -- 8/17/06
- TT-RE -- 1A2 -- -- 4 (SR 080106) -- 8/17/06
- HinLVA-TT -- 1A3 -- -- 5 (SR 080106) --8/17/06
- Hin-TT -- 1A3 -- -- 6 (SR 080106) -- 8/17/06
- Hix-TT-RE -- 1A2 -- -- 7 (SR 080106) -- 8/17/06
- TF-Hix -- 1A2 -- -- 8 (SR 080106) -- 8/17/06
- TF-Hix-TT-RE -- 1A2 -- -- 9 (SR 080106) -- 8/17/06
- Hix-TF-Hix-TT-RE -- 1A2 -- -- 10 (SR 080106) -- 8/17/06
- TB-Hix -- 1A2 -- -- 11 (SR 080106) -- 8/17/06
- TB-Hix-TT-RE -- 1A2 -- -- 12 (SR 080106) -- 8/17/06
- Hix-TB-Hix-RE -- 1A2 -- -- 13 (SR 080106) -- 8/17/06
- TB-RBS -- 1A3 -- -- 15 (SR 080106) -- 8/17/06
- pBad-Hix-TB-RBS -- 1A2 -- -- 16 (SR 080106) -- 8/17/0
- TB-RBS-Hix-TT-RE -- 1A2 -- -- 1 (EZ tet+ 080306) -- 8/17/06
- pLac-Hix -- 1A2 -- -- clone #1 (KH 021707) -- 3/19/07
- RFPrev-RBSrev-hix -- 1A2 -- -- clone #1 (KH 021707) -- 3/19/07
- RFPrev-RBSrev-hix-RBS-TetF -- 1A7 -- -- clone #2 (KH 022807) -- 3/19/07
- pLac-Hix-RBS-TF -- 1A7 -- -- streak (KH 022107) -- 3/19/07
- RBS-TetF -- 1A7 -- N/A -- streak (KH 031507) -- 3/19/07
Pancake Stacks (red)
- pBad-Hix-RBS-TF-Hix-TT-RE -- -- 6 (EZ tet+ 080306)-- 8/17/06
- pBad-Hix-TB-RBS-Hix-TT-RE -- -- 2 (EZ tet+ 080306)-- 8/17/06
- pBad-RBS-Hix-TF-Hix-TT-RE -- -- 8 (EZ tet- 080306)-- 8/17/06
- TT-(1,2) -- 1A3 -- -- N/A --2/19/07
- TT-(1,2) -- 3K3 -- -- N/A -- 2/21/07
- TT-(1,2) -- 4A3 -- N/A -- N/A -- not yet made
- (1,2) HixC-pBad-HixC-RBS-TetF-HixC-TT-RE -- 1A2 -- -- IGEM 2006 Dav. Amp Vectors spot 10 -- 1/29/07
- (-1,-2) HixC-pBadrev-HixC-TB-RBSrev-HixC -- 1A2 -- -- IGEM 2006 Dav. Amp Vectors spot 14 -- 1/29/07
- (-2,-1) HixC-TetB-RBSrev-HixC-pBadrev-HixC -- 1A2-- -- KH 12/14/06 or KH 12/16/06 depending on vector -- 1/29/07
- (1)2 RFPrev-RBSrev-HixC-pLac-HixC-RBS-TetF -- 1A7 -- -- (KH 022807) -- 5/08/07
- (1)-2 RFPrev-RBSrev-HixC-pLac-HixC-TetB-RBSrev -- 1A7 -- -- 2 (KH 032507) -- 5/08/07