The What's and How's

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Revision as of 17:43, 19 June 2007

• What is the list of DNA parts that we will need for the first stage, second stage, entire project?
  
• ERIN and SABRIYA Which DNA parts exist in the registry?Answer1More Parts
  
• ERIN and SABRIYAWhich DNA parts will have to be designed? Will they be synthesized or produced by PCR?
  

loxP sites Hin/Hix CRE gene Degradation Tags

1. [http://www.bio.davidson.edu/courses/Molbio/Protocols/reagents.html Common molecular reagents]
2. [http://partsregistry.org/Assembly:Standard_assembly Standard Assembly]
3. [http://www.bio.davidson.edu/courses/Molbio/Protocols/anneal_oligos.html Building dsDNA with Oligos]
4. [http://www.bio.davidson.edu/courses/Molbio/Protocols/pcr.html Setting up PCR mixtures]
5. [http://www.bio.davidson.edu/courses/Molbio/Protocols/magnesium.html PCR and Mg²⁺ concentration]
6. [http://www.bio.davidson.edu/courses/Molbio/Protocols/Clean_Concentrate.html Clean and Concentrate DNA (after PCR, before digestion)]
7. [http://www.bio.davidson.edu/courses/Molbio/Protocols/pourgel.html Pouring an agarose gel]
8. [http://www.bio.davidson.edu/courses/Molbio/Protocols/molwt.html Calculate MWs]
9. [http://www.bio.davidson.edu/courses/Molbio/Protocols/digestion.html Digest DNA with restriction enzymes]
10. Double Digest Guide
11. [http://www.bio.davidson.edu/courses/Molbio/Protocols/clean_short.html Ethanol Precipitate DNA (short protocol)]
12. [http://www.bio.davidson.edu/courses/Molbio/Protocols/gels2002/1kbladder.pdf 1kb MW markers]
13. [http://www.bio.davidson.edu/courses/Molbio/Protocols/SAP.html Shrimp Alkaline Phosphatase]
14. [http://www.bio.davidson.edu/courses/Molbio/Protocols/Qiagen_gelpure.html Qiagen QIAquick Gel Purification]
15. [http://www.bio.davidson.edu/courses/Molbio/Protocols/QIAQuick_recycle.html Qiagen QIAquick Column Regeneration Protocol]
16. [http://www.bio.davidson.edu/courses/Molbio/Protocols/gelpure.html ElectroElute Gel Purification]
17. [http://www.bio.davidson.edu/courses/Molbio/Protocols/ligation.html Ligation Protocol]
18. [http://www.bio.davidson.edu/courses/Molbio/Protocols/Promegacompcells.pdf Heat Shock Transformation] OR [http://www.bio.davidson.edu/courses/Molbio/Protocols/transformation.html Short version of Heat Shock]
19. [http://www.bio.davidson.edu/courses/Molbio/Protocols/Zippy_Transformation.html Zippy Transformation]
20. [http://www.bio.davidson.edu/courses/Molbio/Protocols/ColonyPCR_Screening.html Colony PCR to Screen for Successful Ligations]
21. [http://www.bio.davidson.edu/courses/Molbio/Protocols/miniprepPrmega.html Promega miniprep]
22. [http://www.bio.davidson.edu/courses/Molbio/Protocols/Tranformation_list.html Choices for Transformation: Heat Shock vs. Zyppy]
23. [http://www.bio.davidson.edu/courses/Molbio/Protocols/MiniPrep_list.html Choices for Mini-Preps: Promega vs. Zyppy]