Freezer Stocks

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{| width=900 border="1"
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'''GROUP (CAP COLOR)'''<br>
-
|-
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'''CONSTRUCT''' -- '''VECTOR''' -- '''PART NO.''' -- '''SOURCE COLONY/ PLATE''' -- '''DATE FROZEN'''<br><br>
-
| '''GROUP (CAP COLOR)''' || '''CONSTRUCT''' || '''PART NO.''' || '''SOURCE COLONY/ PLATE''' || '''DATE FROZEN'''
+
 
-
|-
+
'''Basic Parts''' (<font style="background-color: lightgreen">green</font>)
-
|Basic Parts (green) || Kan backward || <partinfo>BBa_J31002</partinfo> ||IGEM 2006 Dav. Amp Vectors || 1/29/07
+
*pSB1A7 insulated cloning vector -- N/A -- <partinfo>pSB1A7</partinfo> -- IGEM 2006 Dav. Amp  Vectors -- 1/29/07
-
|-
+
*RFP reverse -- 1A3 -- <partinfo>BBa_J31008</partinfo> -- streak clone #1 (KH 020407) -- 2/19/07
-
|Basic Parts (green) || pSB1A7 insulated cloning vector || <partinfo>pSB1A7</partinfo> || IGEM 2006 Dav. Amp  Vectors || 1/29/07
+
*Hin-100 -- 1A2 -- <partinfo>BBa_J31000</partinfo> -- 6 (EZ Basic 080306) -- 8/17/06
-
|-
+
*Hin LVA -- 1A2 -- <partinfo>BBa_J31001</partinfo> -- 10 (EZ Basic 080306) -- 8/17/06
-
|Basic Parts (green) || RFP reverse || <partinfo>BBa_J31008</partinfo> ||3 colony RFP reverse||2/19/07
+
*RE -- 1A2 -- <partinfo>BBa_J3101</partinfo> -- 3 (EZ Basic 080306) -- 8/17/06
-
|-
+
*HixC -- 1A2 -- <partinfo>BBa_J44000</partinfo> -- 4 (EZ Basic 080306) -- 8/17/06
-
|Basic Parts (green) || Hin-100 || <partinfo>BBa_J31000</partinfo> || 6 (EZ Basic 080306) || 8/17/06
+
*pLac promoter -- 1A2 -- <partinfo>BBa_R0010</partinfo> -- SR plate 071206 -- 8/17/06
-
|-
+
*pBad promoter -- 1A3 -- <partinfo>BBa_I13453</partinfo> -- SR plate 071206 -- 8/17/06
-
|Basic Parts (green) || Hin LVA || <partinfo>BBa_J31001</partinfo> || 10 (EZ Basic 080306) || 8/17/06
+
*RBS forward -- 1A2 -- <partinfo>BBa_B0030</partinfo> -- SR plate 071206 -- 8/17/06
-
|-
+
*RBS backward -- 1A2 -- <partinfo>BBa_J44001</partinfo> -- Eckdahl streak -- 8/17/06
-
|Basic Parts (green) || RE || <partinfo>BBa_J3101</partinfo>|| 3 (EZ Basic 080306) || 8/17/06
+
*TT forward -- 1AK3 -- <partinfo>BBa_B0015</partinfo> -- AMC streak 071806 -- 8/17/06
-
|-
+
*Tet forward -- 1A2 -- <partinfo>BBa_J31007</partinfo> -- 11 (EZ Basic 080306) -- 8/17/06
-
|Basic Parts (green) || Hix C || <partinfo>BBa_J44000</partinfo> || 4 (EZ Basic 080306) || 8/17/06
+
*Tet backward -- 1A2 -- <partinfo>BBa_J31006</partinfo> -- 12 (EZ Basic 080306) -- 8/17/06
-
|-
+
*CmR forward -- 1A2 -- <partinfo>BBa_J31005</partinfo> -- 13 (EZ Basic 080306) -- 8/17/06
-
|Basic Parts (green) || pLac promoter || <partinfo>BBa_R0010</partinfo> || SR plate 071206 || 8/17/06
+
*CmR backward -- 1A2 -- <partinfo>BBa_J31004</partinfo> -- 15 (EZ Basic 080306) -- 8/17/06
-
|-
+
*Kan forward -- 1A2 -- <partinfo>BBa_J31003</partinfo> -- 14 (EZ Basic 080306) -- 8/17/06
-
|Basic Parts (green) || pBad promoter || <partinfo>BBa_I13453</partinfo> || SR plate 071206 || 8/17/06
+
*Kan backward -- 1A2? -- <partinfo>BBa_J31002</partinfo> -- IGEM 2006 Dav. Amp Vectors -- 1/29/07
-
|-
+
*pLacrev -- 1A3 -- <partinfo>BBa_J31013</partinfo> -- 1 (KH 040207) -- 5/08/07
-
|Basic Parts (green) || RBS forward || <partinfo>BBa_B0030</partinfo> || SR plate 071206 || 8/17/06
+
----
-
|-
+
'''Intermediates''' (<font style="background-color: yellow">yellow</font>)
-
|Basic Parts (green) || RBS backward || <partinfo>BBa_J44001</partinfo> || Eckdahl streak || 8/17/06
+
*RFPrev-RBSrev -- 1A2 -- <partinfo>BBa_S03644</partinfo> -- streak (KH 021507) -- 2/19/07 (used in subsequent assemblies)
-
|-
+
*RFPrev-RBSrev -- 1A2 -- <partinfo>BBa_J31011</partinfo> -- IGEM 2006 Dav. Amp Vectors spot 3 -- 1/29/07 (not used in subsequent assemblies)
-
|Basic Parts (green) || TT forward || <partinfo>BBa_B0015</partinfo> || AMC streak 071806 || 8/17/06
+
*"AraC-PC, Hin" -- 1K3 -- <partinfo>BBa_J3107</partinfo> -- IGEM 2006 Dav. Kan Vectors spot 4 -- 1/29/07
-
|-
+
*"AraC-PC, HinLVA" -- 1K3 -- <partinfo>BBa_J3108</partinfo> -- IGEM 2006 Dav. Kan Vectors spot 5 -- 1/29/07
-
|Basic Parts (green) || Tet forward || <partinfo>BBa_J31007</partinfo> || 11 (EZ Basic 080306) || 8/17/06
+
*AraCPCrev-TT -- 3K3 -- <partinfo>BBa_S03591</partinfo> -- IGEM 2006 Dav. Kan Vectors spot 6 -- 1/29/07; 5/15/07
-
|-
+
*TT-RBS-TetF -- ? -- <partinfo>BBa_S03595</partinfo> -- IGEM 2006 Dav. Amp Vectors spot 7 -- 1/29/07
-
|Basic Parts (green) || Tet backward || <partinfo>BBa_J31006</partinfo> || 12 (EZ Basic 080306) || 8/17/06
+
*KanB-RBSrev -- 1A3 -- <partinfo>BBa_S03633</partinfo> -- IGEM 2006 Dav. Amp Vectors spot 8 -- 1/29/07
-
|-
+
*RBS-TF-HixC-TT-RE -- ? -- <partinfo>BBa_S03634</partinfo> -- IGEM 2006 Dav. Amp Vectors spot 9 -- 1/29/07
-
|Basic Parts (green) || CmR forward || <partinfo>BBa_J31005</partinfo> || 13 (EZ Basic 080306) || 8/17/06
+
*TB-RBSrev-hix -- ? -- <partinfo>BBa_S03636</partinfo> -- IGEM 2006 Dav. Amp Vectors spot 11 -- 1/29/07
-
|-
+
*Hix-pBadrev-Hix -- ? -- <partinfo>BBa_S03637</partinfo> -- IGEM 2006 Dav. Amp Vectors spot 12 -- 1/29/07
-
|Basic Parts (green) || CmR backward || <partinfo>BBa_J31004</partinfo> || 15 (EZ Basic 080306) || 8/17/06
+
*Hix-pBadrev-Hix-RBS-TF -- ? -- <partinfo>BBa_S03638</partinfo> -- IGEM 2006 Dav. Amp Vectors spot 13 -- 1/29/07
-
|-
+
*Hix-TetB-RBSrev-Hix -- 1A2 -- <partinfo>BBa_S03641</partinfo> -- (KH 121106) -- 1/29/07; 5/08/07
-
|Basic Parts (green) || Kan forward || <partinfo>BBa_J31003</partinfo> || 14 (EZ Basic 080306) || 8/17/06
+
*pBad-RBS -- 1A2 -- <partinfo>BBa_J31002</partinfo> -- 1 (SR 080106) -- 8/17/06
-
|-
+
*pBad-Hix -- 1A2 -- <partinfo>BBa_S03529</partinfo> -- Eckdahl streak -- 8/17/06
-
|Intermediates (yellow) || RFPrev-RBSrev || <partinfo>BBa_S03644</partinfo>||3 colony RFPrev-RBSrev|| 2/19/07
+
*pLac-RBS -- 1A2 -- <partinfo>BBa_S03511</partinfo> -- 14 (SR 080106) -- 8/17/06
-
|-
+
*pBad-Hix-RBS -- 1A2 -- <partinfo>BBa_S03530</partinfo> -- 3 (SR 080106) -- 8/17/06
-
|Intermediates (yellow) || RFPrev-RBSrev || <partinfo>BBa_J31011</partinfo> || IGEM 2006 Dav. Amp Vectors spot 3 || 1/29/07
+
*TT-RE -- 1A2 -- <partinfo>BBa_S03516</partinfo> -- 4 (SR 080106) -- 8/17/06
-
|-
+
*HinLVA-TT -- 1A3 -- <partinfo>BBa_S03514</partinfo> -- 5 (SR 080106) --8/17/06
-
|Intermediates (yellow) || "AraC-PC, Hin" || <partinfo>BBa_J3107</partinfo> || IGEM 2006 Dav. Kan Vectors spot 4 || 1/29/07
+
*Hin-TT -- 1A3 -- <partinfo>BBa_S03515</partinfo> -- 6 (SR 080106) -- 8/17/06
-
|-
+
*Hix-TT-RE -- 1A2 -- <partinfo>BBa_S03522</partinfo> -- 7 (SR 080106) -- 8/17/06
-
|Intermediates (yellow) || "AraC-PC, HinLVA" || <partinfo>BBa_J3108</partinfo>|| IGEM 2006 Dav. Kan Vectors spot 5 || 1/29/07
+
*TF-Hix -- 1A2 -- <partinfo>BBa_S03523</partinfo> -- 8 (SR 080106) -- 8/17/06
-
|-
+
*TF-Hix-TT-RE -- 1A2 -- <partinfo>BBa_S03524</partinfo> -- 9 (SR 080106) -- 8/17/06
-
|Intermediates (yellow) || AraCPCrev-TT || <partinfo>BBa_S03591</partinfo> || IGEM 2006 Dav. Kan Vectors spot 6|| 1/29/07
+
*Hix-TF-Hix-TT-RE -- 1A2 -- <partinfo>BBa_S03527</partinfo> -- 10 (SR 080106) -- 8/17/06
-
|-
+
*TB-Hix -- 1A2 -- <partinfo>BBa_S03525</partinfo> -- 11 (SR 080106) -- 8/17/06
-
|Intermediates (yellow) || TT-RBS-TetF ||<partinfo>BBa_S03595</partinfo> ||IGEM 2006 Dav. Amp Vectors spot 7 || 1/29/07
+
*TB-Hix-TT-RE -- 1A2 -- <partinfo>BBa_S03526</partinfo> -- 12 (SR 080106) -- 8/17/06
-
|-
+
*Hix-TB-Hix-RE -- 1A2 -- <partinfo>BBa_S03528</partinfo> -- 13 (SR 080106) -- 8/17/06
-
|Intermediates (yellow) || KanB-RBSrev|| <partinfo>BBa_S03633</partinfo> || IGEM 2006 Dav. Amp Vectors spot 8 ||1/29/07
+
*TB-RBS -- 1A3 -- <partinfo>BBa_S03532</partinfo> -- 15 (SR 080106) -- 8/17/06
-
|-
+
*pBad-Hix-TB-RBS -- 1A2 -- <partinfo>BBa_S03533</partinfo> -- 16 (SR 080106) -- 8/17/0
-
|Intermediates (yellow) || RBS-TF-HixC-TT-RE ||<partinfo>BBa_S03634</partinfo> || IGEM 2006 Dav. Amp Vectors spot 9 || 1/29/07
+
*TB-RBS-Hix-TT-RE -- 1A2 -- <partinfo>BBa_S03534</partinfo> -- 1 (EZ tet+ 080306) -- 8/17/06
-
|-
+
*pLac-Hix -- 1A2 -- <partinfo>BBa_S03645</partinfo>-- clone #1 (KH 021707) -- 3/19/07
-
|Intermediates (yellow) || TB-RBSrev-hix || <partinfo>BBa_S03636</partinfo> || IGEM 2006 Dav. Amp Vectors spot 11 || 1/29/07
+
*RFPrev-RBSrev-hix -- 1A2 -- <partinfo>BBa_S03648</partinfo> -- clone #1 (KH 021707) -- 3/19/07
-
|-
+
*RFPrev-RBSrev-hix-RBS-TetF -- 1A7 -- <partinfo>BBa_S03649</partinfo> -- clone #2 (KH 022807) -- 3/19/07
-
|Intermediates (yellow) || Hix-pBadrev-Hix || <partinfo>BBa_S03637</partinfo> || IGEM 2006 Dav. Amp Vectors spot 12 || 1/29/07
+
*pLac-Hix-RBS-TF -- 1A7 -- <partinfo>BBa_S03647</partinfo> -- streak (KH 022107) -- 3/19/07
-
|-
+
*RBS-TetF -- 1A7 -- N/A -- streak (KH 031507) -- 3/19/07
-
|Intermediates (yellow) || Hix-pBadrev-Hix-RBS-TF || <partinfo>BBa_S03638</partinfo> || IGEM 2006 Dav. Amp Vectors spot 13 || 1/29/07
+
*pLac-HixC-TetB-RBSrev -- 1A3 -- <partinfo>BBaS03672</partinfo> -- streak (KH 032207) -- 5/08/07
-
|-
+
*RBS-TetF-HixC -- 1A2 -- <partinfo>BBa_S03643</partinfo> -- 1 (KH 013007) -- 5/08/07
-
|Intermediates (yellow) || HixC-TetB-RBSrev-HixC ||<partinfo>BBa_S03641</partinfo> || KH 12/11/06 (plate technically says RBS-TB) || 1/29/07
+
*(-1,2) -- 1A7 -- <partinfo>BBa_S03674</partinfo> -- 1 (KH 020707) -- 5/08/07
-
|-
+
*RFPrev-RBSrev-HixC-pLac-HixC -- 1A7 -- <partinfo>BBa_S03683</partinfo> -- 1 (KH 050107) -- 5/08/07
-
|Intermediates (yellow) || pBad-RBS || <partinfo>BBa_J31002</partinfo> || 1 (SR 080106) || 8/17/06
+
*RFPrev-RBSrev-HixC-RBS-TetF-HixC -- 1A7 -- <partinfo>BBa_S03675</partinfo> -- streak (KH 041107) -- 5/08/07
-
|-
+
*RFPrev-RBSrev-HixC-TetB-RBSrev-HixC -- 1A7 -- <partinfo>BBa_S03676</partinfo> -- streak (KH 041107) -- 5/08/07
-
|Intermediates (yellow) || pBad-Hix || <partinfo>BBa_S03529</partinfo> || Eckdahl streak || 8/17/06
+
*RFPrev-RBSrev-HixC -- 1A7 -- <partinfo>BBa_S03648</partinfo> -- 2 (KH031507) -- 5/08/07
-
|-
+
*pLacrev-HixC -- 1A2 -- <partinfo>BBa_S03678</partinfo> -- 2 (KH 042307) -- 5/08/07
-
|Intermediates (yellow) || pLac-RBS || <partinfo>BBa_S03511</partinfo> || 14 (SR 080106) || 8/17/06
+
*"HinLVAexp" pLac-RBS-HinLVA-TT -- 3K3 -- <partinfo>BBa_S03536</partinfo> -- retransformation (KH 051507) -- 5/17/07
-
|-
+
----
-
|Intermediates (yellow) || pBad-Hix-RBS || <partinfo>BBa_S03530</partinfo> || 3 (SR 080106) || 8/17/06
+
'''Pancake Stacks''' (<font style="background-color: red">red</font>)
-
|-
+
*pBad-Hix-RBS-TF-Hix-TT-RE -- <partinfo>BBa_J3103</partinfo> -- 6 (EZ tet+ 080306)-- 8/17/06
-
|Intermediates (yellow) || TT-RE || <partinfo>BBa_S03516</partinfo> || 4 (SR 080106) || 8/17/06
+
*pBad-Hix-TB-RBS-Hix-TT-RE -- <partinfo>BBa_J3106</partinfo>-- 2 (EZ tet+ 080306)-- 8/17/06
-
|-
+
*pBad-RBS-Hix-TF-Hix-TT-RE -- <partinfo>BBa_J3104</partinfo> -- 8 (EZ tet- 080306)-- 8/17/06
-
|Intermediates (yellow) || HinLVA-TT || <partinfo>BBa_S03514</partinfo> || 5 (SR 080106) ||8/17/06
+
*TT-(1,2) -- 1A3 -- <partinfo>BBa_J31012</partinfo> -- N/A --2/19/07
-
|-
+
*TT-(1,2) -- 3K3 -- <partinfo>BBa_J31012</partinfo> -- N/A -- 2/21/07
-
|Intermediates (yellow) || Hin-TT || <partinfo>BBa_S03515</partinfo> || 6 (SR 080106) || 8/17/06
+
*TT-(1,2) -- 4A3 -- <partinfo>BBa_J31012</partinfo> -- retransformation (KH 022807) -- 5/08/07
-
|-
+
*(1,2) HixC-pBad-HixC-RBS-TetF-HixC-TT-RE -- 1A2 -- <partinfo>BBa_S03635</partinfo> -- IGEM 2006 Dav. Amp Vectors spot 10 -- 1/29/07
-
|Intermediates (yellow) || Hix-TT-RE || <partinfo>BBa_S03522</partinfo> || 7 (SR 080106) || 8/17/06
+
*(-1,-2) HixC-pBadrev-HixC-TB-RBSrev-HixC -- 1A2 -- <partinfo>BBa_S03639</partinfo> -- IGEM 2006 Dav. Amp Vectors spot 14 -- 1/29/07
-
|-
+
*(-1,-2) HixC-pBadrev-HixC-TB-RBSrev-HixC -- 1A7 -- <partinfo>BBa_S03639</partinfo> -- retransformation (KH 020407) -- 5/08/07
-
|Intermediates (yellow) || TF-Hix || <partinfo>BBa_S03523</partinfo> || 8 (SR 080106) || 8/17/06
+
*(-2,-1) HixC-TetB-RBSrev-HixC-pBadrev-HixC -- 1A2-- <partinfo>BBa_S03642</partinfo> -- KH 12/14/06 or KH 12/16/06 depending on vector -- 1/29/07
-
|-
+
* (1)2 RFPrev-RBSrev-HixC-pLac-HixC-RBS-TetF -- 1A7 -- <partinfo>BBa_S03649</partinfo> -- (KH 022807) -- 5/08/07
-
|Intermediates (yellow) || TF-Hix-TT-RE || <partinfo>BBa_S03524</partinfo> || 9 (SR 080106) || 8/17/06
+
* (-1)2 RFPrev-RBSrev-HixC-pLacflipped-HixC-RBS-TetF -- 1A7 -- N/A -- red 1 (KH 031207) -- 5/08/07
-
|-
+
* (1)-2 RFPrev-RBSrev-HixC-pLac-HixC-TetB-RBSrev -- 1A7 -- <partinfo>BBa_S03673</partinfo> -- 2 (KH 032507) -- 5/08/07
-
|Intermediates (yellow) || Hix-TF-Hix-TT-RE || <partinfo>BBa_S03527</partinfo> || 10 (SR 080106) || 8/17/06
+
* (-1)-2 RFPrev-RBSrev-HixC-pLacflipped-HixC-TetB-RBSrev -- 1A7 -- N/A -- (KH 032807) -- 5/08/07
-
|-
+
* RR(2,1) RFPrev-RBSrev-HixC-RBS-TetF-HixC-pLac-HixC -- 1A7 -- <partinfo>BBa_S03677</partinfo> -- (KH 043007) -- 5/08/07
-
|Intermediates (yellow) || TB-Hix || <partinfo>BBa_S03525</partinfo> || 11 (SR 080106) || 8/17/06
+
* RR(2,-1) RFPrev-RBSrev-HixC-RBS-TetF-HixC-pLacrev-HixC -- 1A7 -- <partinfo>BBa_S03679</partinfo> -- (KH 043007) -- 5/08/07
-
|-
+
* RR(-2,1) RFPrev-RBSrev-HixC-TetB-RBSrev-HixC-pLac-HixC -- 1A7 -- <partinfo>BBa_S03680</partinfo> -- (KH 043007) -- 5/08/07
-
|Intermediates (yellow) || TB-Hix-TT-RE || <partinfo>BBa_S03526</partinfo> || 12 (SR 080106) || 8/17/06
+
* RR(-2,-1) RFPrev-RBSrev-HixC-TetB-RBSrev-HixC-pLacrev-HixC -- 1A7 -- <partinfo>BBa_S03681</partinfo> -- (KH 043007) -- 5/08/07
-
|-
+
* RR(1,2) RFPrev-RBSrev-HixC-pLac-HixC-RBS-TetF-HixC -- 1A7 -- <partinfo>BBa_S03684</partinfo> -- (KH 050807) -- 5/08/07
-
|Intermediates (yellow) || Hix-TB-Hix-RE || <partinfo>BBa_S03528</partinfo> || 13 (SR 080106) || 8/17/06
+
* RR(1,-2) RFPrev-RBSrev-HixC-pLac-HixC-TetB-RBSrev-HixC -- 1A7 -- <partinfo>BBa_S03685</partinfo> -- (KH 050807) -- 5/08/07
-
|-
+
* RR(-1,2) RFPrev-RBSrev-HixC-pLacrev-HixC-RBS-TetF-HixC -- 1A7 -- <partinfo>BBa_S03687</partinfo> -- (KH 040607) -- 5/08/07
-
|Intermediates (yellow) || TB-RBS || <partinfo>BBa_S03532</partinfo> || 15 (SR 080106) || 8/17/06
+
* RR(-1,-2) RFPrev-RBSrev-HixC-pLacrev-HixC-TetB-RBSrev-HixC -- 1A7 -- <partinfo>BBa_S03688</partinfo> -- (KH 040607) -- 5/08/07
-
|-
+
-
|Intermediates (yellow) || pBad-Hix-TB-RBS || <partinfo>BBa_S03533</partinfo> || 16 (SR 080106) || 8/17/0
+
-
|-
+
-
|Intermediates (yellow) || TB-RBS-Hix-TT-RE || <partinfo>BBa_S03534</partinfo> || 1 (EZ tet+ 080306) || 8/17/06
+
-
|-
+
-
|Pancake Stacks (red) || pBad-Hix-RBS-TF-Hix-TT-RE || <partinfo>BBa_J3103</partinfo> || 6 (EZ tet+ 080306)|| 8/17/06
+
-
|-
+
-
|Pancake Stacks (red) || pBad-Hix-TB-RBS-Hix-TT-RE || <partinfo>BBa_J3106</partinfo>|| 2 (EZ tet+ 080306)|| 8/17/06
+
-
|-
+
-
|Pancake Stacks (red) || pBad-RBS-Hix-TF-Hix-TT-RE || <partinfo>BBa_J3104</partinfo> || 8 (EZ tet- 080306)|| 8/17/06
+
-
|-
+
-
|Pancake Stacks (red) || TT-(1,2) in 1A3 || N/A || N/A ||2/19/07
+
-
|-
+
-
|Pancake Stacks (red) || TT-(1,2) in 3K3 || N/A || N/A || not yet made
+
-
|-
+
-
|Pancake Stacks (red) || TT-(1,2) in 4A3 || N/A || N/A || not yet made
+
-
|-
+
-
|Pancake Stacks (red) || HixC-pBad-HixC-RBS-TetF-HixC-TT-RE || <partinfo>BBa_S03635</partinfo> || IGEM 2006 Dav. Amp Vectors spot 10 ||1/29/07
+
-
|-
+
-
|Pancake Stacks (red) || HixC-pBadrev-HixC-TB-RBSrev-HixC || <partinfo>BBa_S03639</partinfo> || IGEM 2006 Dav. Amp Vectors spot 14 || 1/29/07
+
-
|-
+
-
|Pancake Stacks (red) || (-2,-1) HixC-TetB-RBSrev-HixC-pBadrev-HixC || <partinfo>BBa_S03642</partinfo> || KH 12/14/06 or KH 12/16/06 depending on vector || 1/29/07
+
-
|}
+

Latest revision as of 19:29, 6 July 2007

GROUP (CAP COLOR)
CONSTRUCT -- VECTOR -- PART NO. -- SOURCE COLONY/ PLATE -- DATE FROZEN

Basic Parts (green)

  • pSB1A7 insulated cloning vector -- N/A -- -- IGEM 2006 Dav. Amp Vectors -- 1/29/07
  • RFP reverse -- 1A3 -- -- streak clone #1 (KH 020407) -- 2/19/07
  • Hin-100 -- 1A2 -- -- 6 (EZ Basic 080306) -- 8/17/06
  • Hin LVA -- 1A2 -- -- 10 (EZ Basic 080306) -- 8/17/06
  • RE -- 1A2 -- -- 3 (EZ Basic 080306) -- 8/17/06
  • HixC -- 1A2 -- -- 4 (EZ Basic 080306) -- 8/17/06
  • pLac promoter -- 1A2 -- -- SR plate 071206 -- 8/17/06
  • pBad promoter -- 1A3 -- -- SR plate 071206 -- 8/17/06
  • RBS forward -- 1A2 -- -- SR plate 071206 -- 8/17/06
  • RBS backward -- 1A2 -- -- Eckdahl streak -- 8/17/06
  • TT forward -- 1AK3 -- -- AMC streak 071806 -- 8/17/06
  • Tet forward -- 1A2 -- -- 11 (EZ Basic 080306) -- 8/17/06
  • Tet backward -- 1A2 -- -- 12 (EZ Basic 080306) -- 8/17/06
  • CmR forward -- 1A2 -- -- 13 (EZ Basic 080306) -- 8/17/06
  • CmR backward -- 1A2 -- -- 15 (EZ Basic 080306) -- 8/17/06
  • Kan forward -- 1A2 -- -- 14 (EZ Basic 080306) -- 8/17/06
  • Kan backward -- 1A2? -- -- IGEM 2006 Dav. Amp Vectors -- 1/29/07
  • pLacrev -- 1A3 -- -- 1 (KH 040207) -- 5/08/07

Intermediates (yellow)

  • RFPrev-RBSrev -- 1A2 -- -- streak (KH 021507) -- 2/19/07 (used in subsequent assemblies)
  • RFPrev-RBSrev -- 1A2 -- -- IGEM 2006 Dav. Amp Vectors spot 3 -- 1/29/07 (not used in subsequent assemblies)
  • "AraC-PC, Hin" -- 1K3 -- -- IGEM 2006 Dav. Kan Vectors spot 4 -- 1/29/07
  • "AraC-PC, HinLVA" -- 1K3 -- -- IGEM 2006 Dav. Kan Vectors spot 5 -- 1/29/07
  • AraCPCrev-TT -- 3K3 -- -- IGEM 2006 Dav. Kan Vectors spot 6 -- 1/29/07; 5/15/07
  • TT-RBS-TetF -- ? -- -- IGEM 2006 Dav. Amp Vectors spot 7 -- 1/29/07
  • KanB-RBSrev -- 1A3 -- -- IGEM 2006 Dav. Amp Vectors spot 8 -- 1/29/07
  • RBS-TF-HixC-TT-RE -- ? -- -- IGEM 2006 Dav. Amp Vectors spot 9 -- 1/29/07
  • TB-RBSrev-hix -- ? -- -- IGEM 2006 Dav. Amp Vectors spot 11 -- 1/29/07
  • Hix-pBadrev-Hix -- ? -- -- IGEM 2006 Dav. Amp Vectors spot 12 -- 1/29/07
  • Hix-pBadrev-Hix-RBS-TF -- ? -- -- IGEM 2006 Dav. Amp Vectors spot 13 -- 1/29/07
  • Hix-TetB-RBSrev-Hix -- 1A2 -- -- (KH 121106) -- 1/29/07; 5/08/07
  • pBad-RBS -- 1A2 -- -- 1 (SR 080106) -- 8/17/06
  • pBad-Hix -- 1A2 -- -- Eckdahl streak -- 8/17/06
  • pLac-RBS -- 1A2 -- -- 14 (SR 080106) -- 8/17/06
  • pBad-Hix-RBS -- 1A2 -- -- 3 (SR 080106) -- 8/17/06
  • TT-RE -- 1A2 -- -- 4 (SR 080106) -- 8/17/06
  • HinLVA-TT -- 1A3 -- -- 5 (SR 080106) --8/17/06
  • Hin-TT -- 1A3 -- -- 6 (SR 080106) -- 8/17/06
  • Hix-TT-RE -- 1A2 -- -- 7 (SR 080106) -- 8/17/06
  • TF-Hix -- 1A2 -- -- 8 (SR 080106) -- 8/17/06
  • TF-Hix-TT-RE -- 1A2 -- -- 9 (SR 080106) -- 8/17/06
  • Hix-TF-Hix-TT-RE -- 1A2 -- -- 10 (SR 080106) -- 8/17/06
  • TB-Hix -- 1A2 -- -- 11 (SR 080106) -- 8/17/06
  • TB-Hix-TT-RE -- 1A2 -- -- 12 (SR 080106) -- 8/17/06
  • Hix-TB-Hix-RE -- 1A2 -- -- 13 (SR 080106) -- 8/17/06
  • TB-RBS -- 1A3 -- -- 15 (SR 080106) -- 8/17/06
  • pBad-Hix-TB-RBS -- 1A2 -- -- 16 (SR 080106) -- 8/17/0
  • TB-RBS-Hix-TT-RE -- 1A2 -- -- 1 (EZ tet+ 080306) -- 8/17/06
  • pLac-Hix -- 1A2 -- -- clone #1 (KH 021707) -- 3/19/07
  • RFPrev-RBSrev-hix -- 1A2 -- -- clone #1 (KH 021707) -- 3/19/07
  • RFPrev-RBSrev-hix-RBS-TetF -- 1A7 -- -- clone #2 (KH 022807) -- 3/19/07
  • pLac-Hix-RBS-TF -- 1A7 -- -- streak (KH 022107) -- 3/19/07
  • RBS-TetF -- 1A7 -- N/A -- streak (KH 031507) -- 3/19/07
  • pLac-HixC-TetB-RBSrev -- 1A3 -- -- streak (KH 032207) -- 5/08/07
  • RBS-TetF-HixC -- 1A2 -- -- 1 (KH 013007) -- 5/08/07
  • (-1,2) -- 1A7 -- -- 1 (KH 020707) -- 5/08/07
  • RFPrev-RBSrev-HixC-pLac-HixC -- 1A7 -- -- 1 (KH 050107) -- 5/08/07
  • RFPrev-RBSrev-HixC-RBS-TetF-HixC -- 1A7 -- -- streak (KH 041107) -- 5/08/07
  • RFPrev-RBSrev-HixC-TetB-RBSrev-HixC -- 1A7 -- -- streak (KH 041107) -- 5/08/07
  • RFPrev-RBSrev-HixC -- 1A7 -- -- 2 (KH031507) -- 5/08/07
  • pLacrev-HixC -- 1A2 -- -- 2 (KH 042307) -- 5/08/07
  • "HinLVAexp" pLac-RBS-HinLVA-TT -- 3K3 -- -- retransformation (KH 051507) -- 5/17/07

Pancake Stacks (red)

  • pBad-Hix-RBS-TF-Hix-TT-RE -- -- 6 (EZ tet+ 080306)-- 8/17/06
  • pBad-Hix-TB-RBS-Hix-TT-RE -- -- 2 (EZ tet+ 080306)-- 8/17/06
  • pBad-RBS-Hix-TF-Hix-TT-RE -- -- 8 (EZ tet- 080306)-- 8/17/06
  • TT-(1,2) -- 1A3 -- -- N/A --2/19/07
  • TT-(1,2) -- 3K3 -- -- N/A -- 2/21/07
  • TT-(1,2) -- 4A3 -- -- retransformation (KH 022807) -- 5/08/07
  • (1,2) HixC-pBad-HixC-RBS-TetF-HixC-TT-RE -- 1A2 -- -- IGEM 2006 Dav. Amp Vectors spot 10 -- 1/29/07
  • (-1,-2) HixC-pBadrev-HixC-TB-RBSrev-HixC -- 1A2 -- -- IGEM 2006 Dav. Amp Vectors spot 14 -- 1/29/07
  • (-1,-2) HixC-pBadrev-HixC-TB-RBSrev-HixC -- 1A7 -- -- retransformation (KH 020407) -- 5/08/07
  • (-2,-1) HixC-TetB-RBSrev-HixC-pBadrev-HixC -- 1A2-- -- KH 12/14/06 or KH 12/16/06 depending on vector -- 1/29/07
  • (1)2 RFPrev-RBSrev-HixC-pLac-HixC-RBS-TetF -- 1A7 -- -- (KH 022807) -- 5/08/07
  • (-1)2 RFPrev-RBSrev-HixC-pLacflipped-HixC-RBS-TetF -- 1A7 -- N/A -- red 1 (KH 031207) -- 5/08/07
  • (1)-2 RFPrev-RBSrev-HixC-pLac-HixC-TetB-RBSrev -- 1A7 -- -- 2 (KH 032507) -- 5/08/07
  • (-1)-2 RFPrev-RBSrev-HixC-pLacflipped-HixC-TetB-RBSrev -- 1A7 -- N/A -- (KH 032807) -- 5/08/07
  • RR(2,1) RFPrev-RBSrev-HixC-RBS-TetF-HixC-pLac-HixC -- 1A7 -- -- (KH 043007) -- 5/08/07
  • RR(2,-1) RFPrev-RBSrev-HixC-RBS-TetF-HixC-pLacrev-HixC -- 1A7 -- -- (KH 043007) -- 5/08/07
  • RR(-2,1) RFPrev-RBSrev-HixC-TetB-RBSrev-HixC-pLac-HixC -- 1A7 -- -- (KH 043007) -- 5/08/07
  • RR(-2,-1) RFPrev-RBSrev-HixC-TetB-RBSrev-HixC-pLacrev-HixC -- 1A7 -- -- (KH 043007) -- 5/08/07
  • RR(1,2) RFPrev-RBSrev-HixC-pLac-HixC-RBS-TetF-HixC -- 1A7 -- -- (KH 050807) -- 5/08/07
  • RR(1,-2) RFPrev-RBSrev-HixC-pLac-HixC-TetB-RBSrev-HixC -- 1A7 -- -- (KH 050807) -- 5/08/07
  • RR(-1,2) RFPrev-RBSrev-HixC-pLacrev-HixC-RBS-TetF-HixC -- 1A7 -- -- (KH 040607) -- 5/08/07
  • RR(-1,-2) RFPrev-RBSrev-HixC-pLacrev-HixC-TetB-RBSrev-HixC -- 1A7 -- -- (KH 040607) -- 5/08/07
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