Sorting Bacteria
From 2006.igem.org
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+ | Back to [[ETH Zurich]] main page. | ||
+ | =Intro= | ||
+ | Emergent sorting. | ||
+ | =Principle= | ||
+ | Two populations of bacteria with different clustering behavior and colors. | ||
+ | |||
+ | =The Aggregation approach= | ||
+ | ==Basic Idea== | ||
The idea would be to have two types of E. coli : | The idea would be to have two types of E. coli : | ||
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This would produce a sorted phenotype (green bacteria surrounded by red bacteria) | This would produce a sorted phenotype (green bacteria surrounded by red bacteria) | ||
+ | |||
+ | |||
+ | ==possible Extensions== | ||
+ | |||
+ | =Discussion= | ||
+ | >> for comments, questions and temporary remarks go to the [[Talk:Sorting_Bacteria]] | ||
+ | |||
+ | Back to [[ETH Zurich]] main page. |
Revision as of 10:23, 4 August 2005
Back to ETH Zurich main page.
Contents |
Intro
Emergent sorting.
Principle
Two populations of bacteria with different clustering behavior and colors.
The Aggregation approach
Basic Idea
The idea would be to have two types of E. coli :
One type would express GFP (they look green)
The other type would express RFP (they look red)
The starting material is an unorganized mixture of red and green bacteria.
The GFP expressing bacteria would start, under induction, to express Antigen 43 (Ag43)
which is an aggregation factor working on stable media culture.
The "green" bacteria would start to gather making green dots on the plates.
The "red" bacteria express, with a certain delay(long enough to let the green ones to
cluster first), the aggregation factor (Ag43).
This would produce a sorted phenotype (green bacteria surrounded by red bacteria)
possible Extensions
Discussion
>> for comments, questions and temporary remarks go to the Talk:Sorting_Bacteria
Back to ETH Zurich main page.