Brown:Summer workshops

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Lab Safety
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[[Brown:iGEM portal 2006 |Home]]<Br>
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[[Calendar:Summer_Workshops|Calendar page]]<br>
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'''Location: Our new lab'''
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<Br>
 +
<Br>
 +
'''Monday June 5, 2006''' (10am-12pm)
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 +
----
 +
Lab Safety <Br>
 +
*clothing/protection
 +
*common sense
 +
*waste
 +
*storage
-
PCR
+
Overview of research basics <Br>
 +
*lab notebook
 +
*resources
 +
*why am I doing this experiment?
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Cloning
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Making stock solutions <Br>
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*pipetting
 +
*weighing
 +
*graduated cylinders
 +
*volumetric flasks
 +
*% solutions (vol/vol, g/vol)
 +
*molar vs. normal solutions
 +
*buffers
 +
 
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HOMEWORK!!! (this will make my workshop shorter)
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*1. You need to make 3% [http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/A2153 BSA] in 10mM [http://www.vwrsp.com/catalog/product/index.cgi?catalog_number=VW0125-3&inE=1&highlight=VW0125-3 Acedic Acid].  Do all the necessary calculations. (If you don't know where to start, don't be discouraged - we'll show you on Monday June 5th).
 +
*2. You need to make 2 liters of the following solution (all are liquids except for the salt): 10% glycerol, 0.9% NaCl, 0.01% Tween 20, 0.01% Tween 80. Show calculations.
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'''Led by:''' Ana (10am-12pm)
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<Br> ''Lab safety training with engineering group from 1pm - 5pm
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'' Please email Karen to join group. All students must have completed lab safety training to work in the lab. <Br> <Br>
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'''Tuesday June 6, 2006''' (10am-12pm)
 +
----
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Restriction digest <Br>
 +
Gel electrophoresis <Br>
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'''Led by:''' Azeem and Jason <Br> <Br>
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'''Thursday June 8, 2006''' (1:00pm - 2:30pm)
 +
----
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Bacteria <Br>
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A tentative list of topics:<Br>
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1. bacterial strains<Br>
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2. how to grow bacteria (solid/liquid culture)
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<Br>3. how to avoid contamination
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<Br>4. how to make competent cells
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<Br>5. how to grow plasmids within bacteria
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<Br>6. antibiotics resistance
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<Br>7. blue/white selection
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<Br>8. iGEM specific information <Br>
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'''Led by:''' Jamie Gagnon <Br> <Br>
 +
 
 +
 
 +
'''Friday June 9, 2006''' (10am-12pm)
 +
 
 +
----
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APE <Br>
 +
'''Led by:''' Azeem and Jason

Latest revision as of 16:38, 8 June 2006

Home
Calendar page
Location: Our new lab

Monday June 5, 2006 (10am-12pm)


Lab Safety

  • clothing/protection
  • common sense
  • waste
  • storage

Overview of research basics

  • lab notebook
  • resources
  • why am I doing this experiment?

Making stock solutions

  • pipetting
  • weighing
  • graduated cylinders
  • volumetric flasks
  • % solutions (vol/vol, g/vol)
  • molar vs. normal solutions
  • buffers

HOMEWORK!!! (this will make my workshop shorter)

  • 1. You need to make 3% [http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/A2153 BSA] in 10mM [http://www.vwrsp.com/catalog/product/index.cgi?catalog_number=VW0125-3&inE=1&highlight=VW0125-3 Acedic Acid]. Do all the necessary calculations. (If you don't know where to start, don't be discouraged - we'll show you on Monday June 5th).
  • 2. You need to make 2 liters of the following solution (all are liquids except for the salt): 10% glycerol, 0.9% NaCl, 0.01% Tween 20, 0.01% Tween 80. Show calculations.

Led by: Ana (10am-12pm)
Lab safety training with engineering group from 1pm - 5pm Please email Karen to join group. All students must have completed lab safety training to work in the lab.

Tuesday June 6, 2006 (10am-12pm)


Restriction digest
Gel electrophoresis
Led by: Azeem and Jason

Thursday June 8, 2006 (1:00pm - 2:30pm)


Bacteria
A tentative list of topics:
1. bacterial strains
2. how to grow bacteria (solid/liquid culture)
3. how to avoid contamination
4. how to make competent cells
5. how to grow plasmids within bacteria
6. antibiotics resistance
7. blue/white selection
8. iGEM specific information
Led by: Jamie Gagnon


Friday June 9, 2006 (10am-12pm)


APE
Led by: Azeem and Jason

Personal tools
Past/present/future years