Brown:Summer workshops
From 2006.igem.org
(Difference between revisions)
| (23 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
| - | '''Monday June 5, 2006''' | + | [[Brown:iGEM portal 2006 |Home]]<Br> |
| + | [[Calendar:Summer_Workshops|Calendar page]]<br> | ||
| + | '''Location: Our new lab''' | ||
| + | <Br> | ||
| + | <Br> | ||
| + | '''Monday June 5, 2006''' (10am-12pm) | ||
---- | ---- | ||
Lab Safety <Br> | Lab Safety <Br> | ||
| + | *clothing/protection | ||
| + | *common sense | ||
| + | *waste | ||
| + | *storage | ||
| + | |||
Overview of research basics <Br> | Overview of research basics <Br> | ||
| + | *lab notebook | ||
| + | *resources | ||
| + | *why am I doing this experiment? | ||
| + | |||
Making stock solutions <Br> | Making stock solutions <Br> | ||
| - | '''Led by:''' | + | *pipetting |
| + | *weighing | ||
| + | *graduated cylinders | ||
| + | *volumetric flasks | ||
| + | *% solutions (vol/vol, g/vol) | ||
| + | *molar vs. normal solutions | ||
| + | *buffers | ||
| + | |||
| + | HOMEWORK!!! (this will make my workshop shorter) | ||
| + | *1. You need to make 3% [http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/A2153 BSA] in 10mM [http://www.vwrsp.com/catalog/product/index.cgi?catalog_number=VW0125-3&inE=1&highlight=VW0125-3 Acedic Acid]. Do all the necessary calculations. (If you don't know where to start, don't be discouraged - we'll show you on Monday June 5th). | ||
| + | *2. You need to make 2 liters of the following solution (all are liquids except for the salt): 10% glycerol, 0.9% NaCl, 0.01% Tween 20, 0.01% Tween 80. Show calculations. | ||
| + | |||
| + | '''Led by:''' Ana (10am-12pm) | ||
<Br> ''Lab safety training with engineering group from 1pm - 5pm | <Br> ''Lab safety training with engineering group from 1pm - 5pm | ||
'' Please email Karen to join group. All students must have completed lab safety training to work in the lab. <Br> <Br> | '' Please email Karen to join group. All students must have completed lab safety training to work in the lab. <Br> <Br> | ||
| - | '''Tuesday June 6, 2006''' | + | '''Tuesday June 6, 2006''' (10am-12pm) |
---- | ---- | ||
Restriction digest <Br> | Restriction digest <Br> | ||
| Line 15: | Line 41: | ||
'''Led by:''' Azeem and Jason <Br> <Br> | '''Led by:''' Azeem and Jason <Br> <Br> | ||
| - | ''' | + | '''Thursday June 8, 2006''' (1:00pm - 2:30pm) |
| - | - | + | |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
---- | ---- | ||
| - | Bacteria | + | Bacteria <Br> |
| - | + | A tentative list of topics:<Br> | |
| - | + | 1. bacterial strains<Br> | |
| + | 2. how to grow bacteria (solid/liquid culture) | ||
| + | <Br>3. how to avoid contamination | ||
| + | <Br>4. how to make competent cells | ||
| + | <Br>5. how to grow plasmids within bacteria | ||
| + | <Br>6. antibiotics resistance | ||
| + | <Br>7. blue/white selection | ||
| + | <Br>8. iGEM specific information <Br> | ||
'''Led by:''' Jamie Gagnon <Br> <Br> | '''Led by:''' Jamie Gagnon <Br> <Br> | ||
| - | '''Friday June 9, 2006''' | + | '''Friday June 9, 2006''' (10am-12pm) |
---- | ---- | ||
| - | + | APE <Br> | |
'''Led by:''' Azeem and Jason | '''Led by:''' Azeem and Jason | ||
Latest revision as of 16:38, 8 June 2006
Home
Calendar page
Location: Our new lab
Monday June 5, 2006 (10am-12pm)
Lab Safety
- clothing/protection
- common sense
- waste
- storage
Overview of research basics
- lab notebook
- resources
- why am I doing this experiment?
Making stock solutions
- pipetting
- weighing
- graduated cylinders
- volumetric flasks
- % solutions (vol/vol, g/vol)
- molar vs. normal solutions
- buffers
HOMEWORK!!! (this will make my workshop shorter)
- 1. You need to make 3% [http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/A2153 BSA] in 10mM [http://www.vwrsp.com/catalog/product/index.cgi?catalog_number=VW0125-3&inE=1&highlight=VW0125-3 Acedic Acid]. Do all the necessary calculations. (If you don't know where to start, don't be discouraged - we'll show you on Monday June 5th).
- 2. You need to make 2 liters of the following solution (all are liquids except for the salt): 10% glycerol, 0.9% NaCl, 0.01% Tween 20, 0.01% Tween 80. Show calculations.
Led by: Ana (10am-12pm)
Lab safety training with engineering group from 1pm - 5pm
Please email Karen to join group. All students must have completed lab safety training to work in the lab.
Tuesday June 6, 2006 (10am-12pm)
Restriction digest
Gel electrophoresis
Led by: Azeem and Jason
Thursday June 8, 2006 (1:00pm - 2:30pm)
Bacteria
A tentative list of topics:
1. bacterial strains
2. how to grow bacteria (solid/liquid culture)
3. how to avoid contamination
4. how to make competent cells
5. how to grow plasmids within bacteria
6. antibiotics resistance
7. blue/white selection
8. iGEM specific information
Led by: Jamie Gagnon
Friday June 9, 2006 (10am-12pm)
APE
Led by: Azeem and Jason
