Brown:Summer workshops
From 2006.igem.org
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| + | [[Brown:iGEM portal 2006 |Home]]<Br> | ||
| + | [[Calendar:Summer_Workshops|Calendar page]]<br> | ||
| + | '''Location: Our new lab''' | ||
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| + | <Br> | ||
'''Monday June 5, 2006''' (10am-12pm) | '''Monday June 5, 2006''' (10am-12pm) | ||
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'' Please email Karen to join group. All students must have completed lab safety training to work in the lab. <Br> <Br> | '' Please email Karen to join group. All students must have completed lab safety training to work in the lab. <Br> <Br> | ||
| - | '''Tuesday June 6, 2006''' | + | '''Tuesday June 6, 2006''' (10am-12pm) |
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Restriction digest <Br> | Restriction digest <Br> | ||
Gel electrophoresis <Br> | Gel electrophoresis <Br> | ||
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'''Led by:''' Azeem and Jason <Br> <Br> | '''Led by:''' Azeem and Jason <Br> <Br> | ||
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2. how to grow bacteria (solid/liquid culture) | 2. how to grow bacteria (solid/liquid culture) | ||
<Br>3. how to avoid contamination | <Br>3. how to avoid contamination | ||
| - | <Br>4. how to grow plasmids within bacteria | + | <Br>4. how to make competent cells |
| - | <Br> | + | <Br>5. how to grow plasmids within bacteria |
| - | <Br> | + | <Br>6. antibiotics resistance |
| - | <Br> | + | <Br>7. blue/white selection |
| + | <Br>8. iGEM specific information <Br> | ||
'''Led by:''' Jamie Gagnon <Br> <Br> | '''Led by:''' Jamie Gagnon <Br> <Br> | ||
| - | '''Friday June 9, 2006''' | + | '''Friday June 9, 2006''' (10am-12pm) |
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| - | + | APE <Br> | |
'''Led by:''' Azeem and Jason | '''Led by:''' Azeem and Jason | ||
Latest revision as of 16:38, 8 June 2006
Home
Calendar page
Location: Our new lab
Monday June 5, 2006 (10am-12pm)
Lab Safety
- clothing/protection
- common sense
- waste
- storage
Overview of research basics
- lab notebook
- resources
- why am I doing this experiment?
Making stock solutions
- pipetting
- weighing
- graduated cylinders
- volumetric flasks
- % solutions (vol/vol, g/vol)
- molar vs. normal solutions
- buffers
HOMEWORK!!! (this will make my workshop shorter)
- 1. You need to make 3% [http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/A2153 BSA] in 10mM [http://www.vwrsp.com/catalog/product/index.cgi?catalog_number=VW0125-3&inE=1&highlight=VW0125-3 Acedic Acid]. Do all the necessary calculations. (If you don't know where to start, don't be discouraged - we'll show you on Monday June 5th).
- 2. You need to make 2 liters of the following solution (all are liquids except for the salt): 10% glycerol, 0.9% NaCl, 0.01% Tween 20, 0.01% Tween 80. Show calculations.
Led by: Ana (10am-12pm)
Lab safety training with engineering group from 1pm - 5pm
Please email Karen to join group. All students must have completed lab safety training to work in the lab.
Tuesday June 6, 2006 (10am-12pm)
Restriction digest
Gel electrophoresis
Led by: Azeem and Jason
Thursday June 8, 2006 (1:00pm - 2:30pm)
Bacteria
A tentative list of topics:
1. bacterial strains
2. how to grow bacteria (solid/liquid culture)
3. how to avoid contamination
4. how to make competent cells
5. how to grow plasmids within bacteria
6. antibiotics resistance
7. blue/white selection
8. iGEM specific information
Led by: Jamie Gagnon
Friday June 9, 2006 (10am-12pm)
APE
Led by: Azeem and Jason
