Plasmid Prep
From 2006.igem.org
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| - | Do plasmid prep on pellets in three tubes labelled "GFP TE" in bottom of left fridge. They are from overnight culture of plasmid I13522 (pTet-GFP). | + | Do plasmid prep on pellets in three tubes labelled "GFP TE" in bottom of left fridge. They are from overnight culture of plasmid I13522 (pTet-GFP). |
Combine the eluted plasmid from all the preps into one tube and measure the yield as follows: | Combine the eluted plasmid from all the preps into one tube and measure the yield as follows: | ||
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# measure A260 of 4 ul plasmid + 76 ul H2O | # measure A260 of 4 ul plasmid + 76 ul H2O | ||
# this measurement gives the concentration in ug/ul, multiply by 1000 to give ng/ul | # this measurement gives the concentration in ug/ul, multiply by 1000 to give ng/ul | ||
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| + | Plasmid prep was done by Brad and Eric (Followed plasmid prep protocol), and I (Kelly) jumped in to help with the A260 readings. We obtained 71 ng/uL. | ||
Latest revision as of 18:55, 19 June 2006
Do plasmid prep on pellets in three tubes labelled "GFP TE" in bottom of left fridge. They are from overnight culture of plasmid I13522 (pTet-GFP).
Combine the eluted plasmid from all the preps into one tube and measure the yield as follows:
- turn on UV spec and blank with H2O
- measure A260 of 4 ul plasmid + 76 ul H2O
- this measurement gives the concentration in ug/ul, multiply by 1000 to give ng/ul
Plasmid prep was done by Brad and Eric (Followed plasmid prep protocol), and I (Kelly) jumped in to help with the A260 readings. We obtained 71 ng/uL.
