Penn State University 2006

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<font color="#FFFFFF" size="2">The bacterial relay race takes advantage of an ability to control cellular motility using inducible promoters such as those involved in nutrient catabolism or quorum sensing. “Receiver” bacteria move in response to small-molecule signals either added to the system or originating from motile, “sender” strains. The most significant challenges relating to this project stem from difficulties of tightly controlling the target motility gene motB. Low levels of motB expression result in system failure (constitutive motility), and resolving this issue is essential to developing reliable modular systems that are the hallmark of synthetic biology.</font>
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<center><h3 style="color: white">Team members</h3></center>
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<font color="#B0C8AE" size="6">>People</font>
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*[[user:jbad|<font color="#99cccc">Jon Bad</font>]] 
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<h2 style="color: #C5D1D7">'''Undergraduate Team'''</h2>
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*[[user:cbuck|<font color="#99cccc">Chris Buck</font>]]
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*[[user:jgrim|<font color="#99cccc">Joel Grim</font>]] 
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*[[user:RSand|<font color="#99cccc">Ralphy</font>]]
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*[[user:ttasc|<font color="#99cccc">Tony</font>]] 
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*[[user:ljw|<font color="#99cccc">LJ</font>]]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Jon Badalamenti]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Lucien Weiss]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Chris Buckno]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Joel Q. Grim</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Ralph Sandoval</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Barbara Andersen</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Jen Dunning</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Natalie Shewski</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">John Bigus</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Aleksandr Lewicki</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Elizabeth Morin</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Yan Shipelskiy</font>]
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<h2 style="color: #C5D1D7">'''Affiliated Grad Students'''</h2>
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Steve Walker</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Deepti Tanjore</font>]
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<center><h3 style="color: white">Faculty</h3></center>
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<h2 style="color: #C5D1D7">'''Faculty'''</h2>
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Tom Richard</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Pat Cirino</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Will Hancock</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Darryl Farber</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Ming Tien</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Paul Weiss</font>]
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*[[user:Pcir|<font color="#99cccc">Pat Cirino</font>]] 
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<h2 style="color: #C5D1D7">'''Graduated Team Members'''</h2>
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*[[user:Whan|<font color="#99cccc">Will Hancock</font>]]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Lane J. Weaver</font>]
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*[[user:TRich|<font color="#99cccc">Tom Richard</font>]] 
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Tony Tascone</font>]
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*[[user:Dfarb|<font color="#99cccc">Darryl Farber</font>]]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Steve Neuman</font>]
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*[[user:MTien|<font color="#99cccc">Ming Tien</font>]] 
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*[[user:PW|<font color="#99cccc">Paul Weiss</font>]]|
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<h2 style="color: #C5D1D7">'''Photos'''</h2>
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Lab Photos</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#FFFFFF">Team Member Photos</font>] 
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<center><h3 style="color: white">Project</h3></center>
 
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*[http://2006.igem.org/wiki/index.php/Penn_State_2005 Penn State iGEM 2005]
 
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*[[Penn_State_University_2006:PSUprotocols|<font color="#99cccc">Protocols</font>]]
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**[[Penn_State_University_2006:Cloning|Cloning]]
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***[[Penn_State_University_2006:Preparing chemically competent cells|Preparing chemically competent cells]] 
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***[[Penn_State_University_2006:Restriction|Restriction]]
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***[[Penn_State_University_2006:Ligation|Ligation]]
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***[[Penn_State_University_2006:Transformation|Transformation]]
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**[[Penn_State_University_2006:Plate-Reading|Plate-Reading]]
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**[[Penn_State_University_2006:Microscopy|Microscopy]]
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**[[Penn_State_University_2006:Motility-assays|Motility-assays]]
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**[[Penn_State_University_2006:Microchannel|Microchannel]]
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**[[Penn_State_University_2006:Strain Construction|Strain Construction]] 
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*[[Penn_State_University_2006:PSUinventory|<font color="#99cccc">Inventory</font>]] 
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<center><h3 style="color: white">Schedule/Progress</h3></center>
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<font color="#646B86" size="6">>Project</font>
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<center>[[Image:Psulogo.JPG]]</center>
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<h2 style="color: #D16349">'''Publications'''</h2>
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#000000">Synthetic Sports: A Bacterial Relay Race</font>]
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<h2 style="color: #D16349">'''Protocols'''</h2>
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#000000">Cloning</font>]
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**[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#000000">Preparing chemically competent cells</font>]
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**[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#000000">Growing Parts</font>]
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**[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#000000">Restriction</font>]
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**[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#000000">Ligation</font>]
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**[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#000000">Transformation</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#000000">Plate-reading</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#000000">Motility-assays</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#000000">Microchannel</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#000000">Strain Construction</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#000000">Inventory</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#000000">DNA Gel</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#000000">Growth Media</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#000000">LB Plates</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#000000">Eiken Agar Plates</font>]
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<h2 style="color:#D16349">'''Progress'''</h2>
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#000000">Submitted Registry Parts</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#000000">Strain-Construction Progress</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#000000">Microchannels results</font>]
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*[http://openwetware.org/wiki/IGEM:PennState/2006<font color="#000000">Motility results</font>]
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<h2 style="color: #D16349">'''Background'''</h2>
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<center><h3 style="color: white">Background/Links</h3></center>
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*[[http://openwetware.org/wiki/IGEM:PennState/2006|<font color="#000000">Development of the Ecoli Relay Race</font>]]
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<center>[[Image:Psulogo.JPG]]</center>
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*[http://en.wikipedia.org/wiki/Relay_race Wikipedia: Relay Race]
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<h2 style="color: #D16349">'''Links'''</h2>
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*[[iGEM|iGEM on OpenWetWare]]
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*[http://partsregistry.org Parts registry]
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*[http://2007.igem.org iGEM wiki]
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*[http://2006.igem.org/wiki/index.php/Penn_State_2005 Penn State iGEM 2005]
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*[http://2006.igem.org/wiki/index.php/Penn_State_University_2006 Penn State iGEM 2006]

Latest revision as of 20:07, 9 March 2007

PSU banner-logo1.jpg Oldmain3.jpg

The bacterial relay race takes advantage of an ability to control cellular motility using inducible promoters such as those involved in nutrient catabolism or quorum sensing. “Receiver” bacteria move in response to small-molecule signals either added to the system or originating from motile, “sender” strains. The most significant challenges relating to this project stem from difficulties of tightly controlling the target motility gene motB. Low levels of motB expression result in system failure (constitutive motility), and resolving this issue is essential to developing reliable modular systems that are the hallmark of synthetic biology.

>People

Undergraduate Team

  • [http://openwetware.org/wiki/IGEM:PennState/2006Jon Badalamenti]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Lucien Weiss]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Chris Buckno]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Joel Q. Grim]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Ralph Sandoval]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Barbara Andersen]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Jen Dunning]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Natalie Shewski]
  • [http://openwetware.org/wiki/IGEM:PennState/2006John Bigus]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Aleksandr Lewicki]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Elizabeth Morin]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Yan Shipelskiy]

Affiliated Grad Students

  • [http://openwetware.org/wiki/IGEM:PennState/2006Steve Walker]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Deepti Tanjore]

Faculty

  • [http://openwetware.org/wiki/IGEM:PennState/2006Tom Richard]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Pat Cirino]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Will Hancock]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Darryl Farber]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Ming Tien]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Paul Weiss]

Graduated Team Members

  • [http://openwetware.org/wiki/IGEM:PennState/2006Lane J. Weaver]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Tony Tascone]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Steve Neuman]

Photos

  • [http://openwetware.org/wiki/IGEM:PennState/2006Lab Photos]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Team Member Photos]



>Project

Publications

  • [http://openwetware.org/wiki/IGEM:PennState/2006Synthetic Sports: A Bacterial Relay Race]

Protocols

  • [http://openwetware.org/wiki/IGEM:PennState/2006Cloning]
    • [http://openwetware.org/wiki/IGEM:PennState/2006Preparing chemically competent cells]
    • [http://openwetware.org/wiki/IGEM:PennState/2006Growing Parts]
    • [http://openwetware.org/wiki/IGEM:PennState/2006Restriction]
    • [http://openwetware.org/wiki/IGEM:PennState/2006Ligation]
    • [http://openwetware.org/wiki/IGEM:PennState/2006Transformation]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Plate-reading]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Motility-assays]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Microchannel]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Strain Construction]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Inventory]
  • [http://openwetware.org/wiki/IGEM:PennState/2006DNA Gel]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Growth Media]
  • [http://openwetware.org/wiki/IGEM:PennState/2006LB Plates]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Eiken Agar Plates]

Progress

  • [http://openwetware.org/wiki/IGEM:PennState/2006Submitted Registry Parts]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Strain-Construction Progress]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Microchannels results]
  • [http://openwetware.org/wiki/IGEM:PennState/2006Motility results]

Background

Links

  • iGEM on OpenWetWare
  • [http://partsregistry.org Parts registry]
  • [http://2007.igem.org iGEM wiki]
  • [http://2006.igem.org/wiki/index.php/Penn_State_2005 Penn State iGEM 2005]
  • [http://2006.igem.org/wiki/index.php/Penn_State_University_2006 Penn State iGEM 2006]

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