Toronto

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'''Welcome to the Blue Water Wiki'''
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== '''Welcome to the Blue Water Wiki''' ==
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http://individual.utoronto.ca/nazareth/iGEM2005/Team.gif <br>
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http://individual.utoronto.ca/nazareth/iGEM2005/BlueWater.gif
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== Project Overview ==
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Good news, there is an E coli strain availabe that contains Tn10 inserted into LacZ, meaning it is lacZ- and Tetracycline sensitive.
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[http://individual.utoronto.ca/nazareth/iGEM2005/wiki.html iGEM Project Overview]
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Here’s an overview of the project.  Currently the write up is an external link to an html, eventually it will be converted to wiki form to allow easier modification.
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about the Tet operon and Tn10
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[http://individual.utoronto.ca/nazareth/iGEM2005/Model.pdf Cell-See-Us Thermometer Model]
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http://www.biomedcentral.com/1471-2180/5/1
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Here’s a model of the Cell-See-Us Thermometer, including the governing system of differential equations.
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-Hannah
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[http://individual.utoronto.ca/nazareth/iGEM2005/ModelResults.pdf Cell-See-Us Thermometer Model Results]
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And here are the results of the model, after values for the constants were plugged in.
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[http://www.math.uwaterloo.ca/~mscott/BBa_J11014.jpg Temperature sensor],
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[http://www.math.uwaterloo.ca/~mscott/BBa_J11018.jpg Temperature sensor with positive feedback]
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The original designs for the temperature sensor, parts BBa_J11014 and BBa_J11018 are available above, along with modeling data. (These files are jpegs, and considerably smaller than the pdfs above).
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UPdate ~ 12 Oct 2005
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Note that the design has since been modified to accommodate minimal parts, called BBa_J11021 and BBa_J11022. The model results for these new designs will be posted shortly.
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== iGEM 2005 Jamboree ==
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15:54, 3 Oct 2005 (EDT)15:54, 3 Oct 2005 (EDT)~~
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[http://individual.utoronto.ca/nazareth/iGEM2005/iGEM.ppt Team Blue Water's iGEM 2005 Presentation]
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Hi everyone,
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Here are the slides we used in our presentation.
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I made a biobrick version of my system, http://parts2.mit.edu/r/parts/partsdb/view.cgi?part_id=6153
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It is part # BBa_J11030
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I hope all the parts are available...
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Also very important NOTE that this system MUST MUST MUST be in LacZ- bugs!
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So lets try and get a hold of those if we want to make my system
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Hannah
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[http://2006.igem.org/wiki/index.php/Jamboree iGEM 2005 Jamboree]
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Here is the wiki for the iGEM 2005 Jamboree that was held November 5 & 6 2005.  The Jamboree was attended by four U of T/Waterloo group members: Prof. Stephen Davies, Matt Scott, Hannah Fong and Emanuel Nazareth.
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[http://www.nature.com Check E, "Synthetic biology: Designs on life," Nature. Nov 24 2005. 438:417-418]
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Here is a feature in Nature about the iGEM 2005 Jamboree.
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[http://pubs.acs.org/cgi-bin/archive.cgi/chreay/2000/100/i07/pdf/cr990115p.pdf Genetically engineered whole-cell sensing systems: coupling biological recognition with reporter genes.]
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== Team Members ==
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Chem Rev. 2000 Jul 12;100(7):2705-38.
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'''Undergraduate Students'''<br>
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Daunert S, Barrett G, Feliciano JS, Shetty RS, Shrestha S, Smith-Spencer W.
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Aman Arya, Biology (Waterloo)<br>
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Joseph Chan, Molecular Genetics (Toronto)<br>
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Hannah Fong, Immunology / Chemistry (Toronto)<br>
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Lutz Kummer, visiting Biology (Waterloo)<br>
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Emanuel Nazareth, Biomedical Engineering (Toronto)<br>
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Martina Spranger, visiting Biology (Waterloo)<br>
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Max Wong, Biomedical Engineering (Toronto)<br>
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Discusses important biosensing systems, mainly in E. coli.
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'''Graduate Students'''<br>
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Seema Nagaraj, IBBME and Dept. of Electrical Engineering (Toronto)<br>
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Matt Scott, Dept. of Applied Mathematics (Waterloo)<br>
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Cindy Ying Yin Yip, IBBME (Toronto)<br>
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On another note, currently if anyone wants to post files (Powerpoint presentations, papers, etc.) the file must be available online somewhere and linked to from this Wiki.  If you need a file to be posted online, feel free to email it to me at emanuelnazareth@hotmail.com and I'll get back to you with how to link to it.
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'''Technical Support'''<br>
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Andrew Hessel, Lab Manager, Mak Lab, University Health Network<br>
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~ Emanuel
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'''Faculty Advisors'''<br>
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Prof. Stephen Davies, IBBME and Dept. of Electrical Engineering (Toronto)<br>
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Prof. Brian Ingalls, Dept. of Applied Mathematics (Waterloo)<br>

Latest revision as of 07:51, 25 November 2005

Contents

Welcome to the Blue Water Wiki

http://individual.utoronto.ca/nazareth/iGEM2005/Team.gif
http://individual.utoronto.ca/nazareth/iGEM2005/BlueWater.gif

Project Overview

[http://individual.utoronto.ca/nazareth/iGEM2005/wiki.html iGEM Project Overview] Here’s an overview of the project. Currently the write up is an external link to an html, eventually it will be converted to wiki form to allow easier modification.

[http://individual.utoronto.ca/nazareth/iGEM2005/Model.pdf Cell-See-Us Thermometer Model] Here’s a model of the Cell-See-Us Thermometer, including the governing system of differential equations.

[http://individual.utoronto.ca/nazareth/iGEM2005/ModelResults.pdf Cell-See-Us Thermometer Model Results] And here are the results of the model, after values for the constants were plugged in.

[http://www.math.uwaterloo.ca/~mscott/BBa_J11014.jpg Temperature sensor], [http://www.math.uwaterloo.ca/~mscott/BBa_J11018.jpg Temperature sensor with positive feedback] The original designs for the temperature sensor, parts BBa_J11014 and BBa_J11018 are available above, along with modeling data. (These files are jpegs, and considerably smaller than the pdfs above).

Note that the design has since been modified to accommodate minimal parts, called BBa_J11021 and BBa_J11022. The model results for these new designs will be posted shortly.

iGEM 2005 Jamboree

[http://individual.utoronto.ca/nazareth/iGEM2005/iGEM.ppt Team Blue Water's iGEM 2005 Presentation] Here are the slides we used in our presentation.

[http://2006.igem.org/wiki/index.php/Jamboree iGEM 2005 Jamboree] Here is the wiki for the iGEM 2005 Jamboree that was held November 5 & 6 2005. The Jamboree was attended by four U of T/Waterloo group members: Prof. Stephen Davies, Matt Scott, Hannah Fong and Emanuel Nazareth.

[http://www.nature.com Check E, "Synthetic biology: Designs on life," Nature. Nov 24 2005. 438:417-418] Here is a feature in Nature about the iGEM 2005 Jamboree.

Team Members

Undergraduate Students
Aman Arya, Biology (Waterloo)
Joseph Chan, Molecular Genetics (Toronto)
Hannah Fong, Immunology / Chemistry (Toronto)
Lutz Kummer, visiting Biology (Waterloo)
Emanuel Nazareth, Biomedical Engineering (Toronto)
Martina Spranger, visiting Biology (Waterloo)
Max Wong, Biomedical Engineering (Toronto)

Graduate Students
Seema Nagaraj, IBBME and Dept. of Electrical Engineering (Toronto)
Matt Scott, Dept. of Applied Mathematics (Waterloo)
Cindy Ying Yin Yip, IBBME (Toronto)

Technical Support
Andrew Hessel, Lab Manager, Mak Lab, University Health Network

Faculty Advisors
Prof. Stephen Davies, IBBME and Dept. of Electrical Engineering (Toronto)
Prof. Brian Ingalls, Dept. of Applied Mathematics (Waterloo)

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