Mississippi State University 2006
From 2006.igem.org
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* We built the following two constructs:<br> | * We built the following two constructs:<br> | ||
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<h3>Results</h3> | <h3>Results</h3> | ||
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+ | * Part BBa_J43000 | ||
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+ | * No Hydrogen, No tetR = fluorescence | ||
+ | * No Hydrogen, with tetR = Repressed Transcription at R0040 tetR, no fluorescence (except for some leakage). | ||
+ | * Hydrogen + tetR = start trqanscription at hybB, produce tetR, increased repression of operon at R0040 tetR. no fluorescence. | ||
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+ | * Part BBa_J43001 | ||
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+ | * No Hydrogen, No inducer = no fluorescence (except for a bit of leakage possibly). | ||
+ | * No Hydrogen, Inducer = Start Transcription at lacZ+PL, fluoresce. | ||
+ | * Hydrogen + Inducer = start transcription at hybB, produce lacI, competitive inhibition of operon at lacZ+PL. Less fluorescence transcribed. | ||
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<h3>Discussion</h3> | <h3>Discussion</h3> | ||
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+ | * Complications: | ||
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+ | * BBa_J43000 was a failed part, we learned during completion of the construction and testing that there was a flaw in the design of the composite part. Although the hybB promoter does appear to detect hydrogen, the YFP is inhibited with the addition of tetR. Therefore, we cannot detect a change in fluorescence when hydrogen is added. | ||
+ | * For the BBa_J43001, calculated error provides a possible explaination for why 100% hydrogen concentration fluoresced more than 75% hydrogen concentration. More testing required to determine the 100% hydrogen result. | ||
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+ | * Achievements: | ||
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+ | * The results of our test agree with possible success for part BBa_J43001. More testing must be performed to further prove our part as a hydrogen detector. | ||
+ | * Our machine confirms the success of hybB, BBa_Q04121, and BBa_E0430 as a composite part | ||
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Latest revision as of 19:33, 30 October 2006
The TeamFaculty Members:
Students:
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Introduction
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H2 Reporter
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Results
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Discussion
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Accomplishments
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