Prairie View A&M University 2006

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== Students ==
== Students ==
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[[Image:PVAMUIGEM.jpg]]
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[[Image:IGEM-Team2k6-sml.jpg ]]
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More Group Pictures
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[[More Group Pictures]]
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[[Image:Group2K6.jpg]]
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[[Image:PVIGEM2K6.jpg]]
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== Advisors ==
== Advisors ==
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www.raulcuerobiotech.com
www.raulcuerobiotech.com
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'''Dr Dierdre Vaden'''
 
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[[Image:DrVaden.jpg]]
 
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Dr Vaden's is  a Genetics Proffeor her work is mainly in yeast genetics and phospholipids metabolism.
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'''Dr Harriet Howard-Lee Block'''
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[[Image:Lee.jpg]]
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'''Dr Harriet Howard-Lee Block'''
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[[Dr Block]]
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[[Image:Lee.jpg]]
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'''Dr Dierdre Vaden'''
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Department Head of Biology
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[[Image:DrVaden.jpg]]
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Area of Interest: Molecular Biology
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[[Dr Vaden]] 
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'''Dr Huajan Fan'''
'''Dr Huajan Fan'''
[[Image:DrFan.jpg]]
[[Image:DrFan.jpg]]
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Assistant Professor in Chemistry.
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[[Dr Fan]]
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== Student Profiles ==
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[[Laina]] | [[Leslie]] | [[Christian]] | [[George]] | [[Oludayo]] | [[Jessica]] | [[Terry]]|[[Fredrick]] | [[Victor]]
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Face File [[https://static.igem.org/mediawiki/2006/2/20/Pictures.ppt]]
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== Project ==
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== Project==
 
(We have changed the title of our project so many times)
(We have changed the title of our project so many times)
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'''Tri-Metallic Biosensor'''[[Link title]]
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'''Tri-Metallic Biosensor'''- PVAMU [[https://static.igem.org/mediawiki/2006/b/b9/Slide_Show2a.ppt]]
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'''Background'''
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There exists two essential needs which enabled us to design the trimetallic probe. The need for alternative forms of oil, in fact their methods and the bioremediation of metals ions from the environment.
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Micrococcus luteus strain (ATCC 4698) was transformed by plasmid pUC57-S-3M, in which Fe (II), Ni (II) and V (II) were fused to the fluorescence proteins (ECFP, EYFP, and mRFP respectively and to a fluorescent protein coding region [C0061(lux I)] ligated with a signaling sequence BBa_I13207.  
Micrococcus luteus strain (ATCC 4698) was transformed by plasmid pUC57-S-3M, in which Fe (II), Ni (II) and V (II) were fused to the fluorescence proteins (ECFP, EYFP, and mRFP respectively and to a fluorescent protein coding region [C0061(lux I)] ligated with a signaling sequence BBa_I13207.  
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Favorite Biobrick Map(Upcoming)
The pUC57-S-3M was standardized for its specific sensoribility response to three metal ions and to sulfur (S), in order to detect metal contamination and/or hydrocarbons associated to these metals. Single and combination of the tree metals were used at different concentrations (0.2, 2, 50 ppm). The pUC57-S-3M was grown in the presence and absence of oxygen and/or hydrocarbon (Thiophenol). Only results from combined metals are reported. The biosensoribility was determined by the response of the pUC57-S-3M to the different concentrations of the metals. This response was measured by bioluminescence, fluorescence, DNA concentration, bacterial growth. These parameters were related to sensoribility of the pUC57-S-3M.
The pUC57-S-3M was standardized for its specific sensoribility response to three metal ions and to sulfur (S), in order to detect metal contamination and/or hydrocarbons associated to these metals. Single and combination of the tree metals were used at different concentrations (0.2, 2, 50 ppm). The pUC57-S-3M was grown in the presence and absence of oxygen and/or hydrocarbon (Thiophenol). Only results from combined metals are reported. The biosensoribility was determined by the response of the pUC57-S-3M to the different concentrations of the metals. This response was measured by bioluminescence, fluorescence, DNA concentration, bacterial growth. These parameters were related to sensoribility of the pUC57-S-3M.
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== Project Planning==
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'''Parts used'''
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<partinfo>BBa_S01984</partinfo>
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<partinfo>BBa_I13908</partinfo>/
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<partinfo>BBa_I13519</partinfo>/
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<partinfo>BBa_I4204</partinfo>/
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<partinfo>BBa_C0061</partinfo>/
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<partinfo>BBa_E0422</partinfo>/
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<partinfo>BBa_E0430</partinfo>/
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<partinfo>BBa_I13207</partinfo>/
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<partinfo>BBa_I13507</partinfo>/
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'''Favorite Parts/ A promoter Biosenser <partinfo>BBa_J48108</partinfo>
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<partinfo>BBa_J48108 specifiedcomponents</partinfo>
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== Project Planning Diary ==
'''Calender'''
'''Calender'''
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'''June'''
'''June'''
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No entry
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'''July'''  
'''July'''  
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'''September'''
'''September'''
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-So far we have tried to isolate several plasmids but results have not been good.  So far we have successfully amplified and isolated  4 plasmids.  
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-So far we have tried to isolate several plasmids but results have not been good.  So far we have successfully amplified and isolated  4 plasmids. Well no one will believe how much we got done in such a small amout of time.  [Laina's Diary]
'''October'''
'''October'''
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Determined that we will base our sensor on the concentrations of three metals ions, Vanadium, Iron and Nickel,and including elemental sulfur. Our focus is on the DNA expression, determined by luminesence and flourescence.
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Ou
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== '''References''' ==
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== Student Profiles ==
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Laina
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[[Image:Laina.jpg]]
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Laina P. Magaya undergraduate Biology Major at Prairie View A&M University.Currently working on Microbial Biotechnology applied to the environment at Prairie View A&M University under the Principal Inevstigator Dr R. Cuero. I have also worked in another project where I learnt '''microbiology techniques''' , determing the effects of arabinose and glucose on growth of ''E. coli'' to determine the expression of ulcer causing agent, ''Helicobacter pylori'' at Texas Woman's University. These experiences will nake me suitable candidate for the synthetic biology project with the Prairie View 2006 iGEM team.
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Lesli
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[[Image:Leslie Pic.jpg]]
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Lesli Caulfield undergraduate Chemical Engineering senior at Prairie View A&M University in Prairie View, TX. I have carried out research in Biosensors and Cell Adhesion at the University of Oklahoma ( summer of 2003, under Dr. David Schmidtke). I have done research in Characterization of Chiral Sulfoxidation by HRP at the University of Kansas REU (CEBC, summer 20005, under Dr. Aaron Scurto). Currently, I am working on Solid State and Submerged Fermentation Systems at Prairie View A&M-CEBC under Dr. Michael Gyamerah with four of my peers. As one can see, I have a diverse and unique research background.  I am interested in Dr. Cuero’s research because it will allow me to not only enhance my research opportunities a bit further, but it will allow the creative side of me to be nurtured and encouraged.  Being that he is one of the elite researchers’, I know I will learn a lot and excel in the iGEM team.
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Christian
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[[Image:Chris2.jpg]]
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My name is Christian Cruz, I am a graduate student of chemical engineering at Prairie View A&M university. I have been working with Dr. Raul Cuero for the last two years,carrying out experiments related to electrochemistry and now using molecular biology techniques. Now with iGEM 2006 I see a chance for myself to put in practice all of my engineering knowledge and molecular experiences.
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Oludayo
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[[Image:olu.jpg]]
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Olu is a Senior Biology Major and the SGA president of Prairie View A&M.
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Fredrick
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[[Image:Fred.jpg]]
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My name is Fredrick, a Senior Architecture student at Prairie View A & M University.  I had the opportunity to travel overseas and apply all that I've learned at Prairie View.  It broaden my views in pursuing other avenues that complimented architecture.  I'm looking forward to getting opportunity to venture off after finishing college.
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George
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[[Image:george.jpg]]
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George D. LaBlanche, a junior Animal Science major at Prairie View A & M University, is a native Texan of Houston and is now most privileged and occasioned, to investigate an alternative treatment and cure with International Distinguished Professor Dr. Raul G. Cuero Ph.D. We intend to investigate the treatment and cure with regards to Heartworms and other internal parasites; with probable human applications. The potential effect of internal organ regeneration is also also to be studied.
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Terry Kommany
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1.Cuero, R, Oullet  T. Metal ions modulate gene expression and accumulation of the mycotoxins aflatoxin and zearalenone.J Appl Microbiol. 2005;98(3):598-605. �
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[[Image:terry.jpg]]
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2.Lovley, DR (2003). "Cleaning up with genomics: applying molecular biology to bioremediation". NATURE REVIEWS. MICROBIOLOGY. 1 (1): 35–44. PMID 15040178.
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Holmes et al 1993b. Holmes D.S et al, 1993. Development of Biosensor to Measure Metal Ions
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Victor Cuero
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3. Bioavailability in mining and metal waste.pp659-666. In Biohydrometallurgical Technologies. Vol. II. A.E Torma, M.L Apel and C.L Brierle,TMS, Warrendale, PA,USA.Eds.
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[[Image:victor.jpg]]
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4.Holmes, D.S. Dubey, U.K and Gongolli, 1993. Development of Biosensor for the detection of Mercury andCopper Ions.Environ, Geochem. Health 16: 229-233.
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Victor is a sophomore in Computer Science Major at Prairie A&M university.
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5.Leonard G. Davis, Mark D. Dibner, James F Battey. Basic Methods in Molecular Biology. Elsevier Science Publishers.1986, New York, Amsterdam and London.

Latest revision as of 11:37, 4 November 2006

Contents

Students

IGEM-Team2k6-sml.jpg

More Group Pictures

Advisors

Dr Raul Cuero,(PhD)

DRcuero.jpg

Dr. Raul Cuero, (PhD), Microbiologist, Distinguished Professor.Research Leader, Scientist and Contact Person for Prairie View A&M University iGEM 2006 group. Dr Cuero holds 8 scientific inventions and more than 90 scientific publications.Dr Cuero is currently working on fungal toxins at the molecular level, and on biogenesis. He is also the author of the book "Triumph and Survival".

Email: olimpa@aol.com

www.raulcuerobiotech.com


Dr Harriet Howard-Lee Block

Lee.jpg

Dr Block


Dr Dierdre Vaden

DrVaden.jpg

Dr Vaden


Dr Huajan Fan

DrFan.jpg

Dr Fan

Student Profiles

Laina | Leslie | Christian | George | Oludayo | Jessica | Terry|Fredrick | Victor

Face File [[1]]

Project

(We have changed the title of our project so many times)

Tri-Metallic Biosensor- PVAMU [[2]]

Background

There exists two essential needs which enabled us to design the trimetallic probe. The need for alternative forms of oil, in fact their methods and the bioremediation of metals ions from the environment.


Micrococcus luteus strain (ATCC 4698) was transformed by plasmid pUC57-S-3M, in which Fe (II), Ni (II) and V (II) were fused to the fluorescence proteins (ECFP, EYFP, and mRFP respectively and to a fluorescent protein coding region [C0061(lux I)] ligated with a signaling sequence BBa_I13207.

Favorite Biobrick Map(Upcoming)

The pUC57-S-3M was standardized for its specific sensoribility response to three metal ions and to sulfur (S), in order to detect metal contamination and/or hydrocarbons associated to these metals. Single and combination of the tree metals were used at different concentrations (0.2, 2, 50 ppm). The pUC57-S-3M was grown in the presence and absence of oxygen and/or hydrocarbon (Thiophenol). Only results from combined metals are reported. The biosensoribility was determined by the response of the pUC57-S-3M to the different concentrations of the metals. This response was measured by bioluminescence, fluorescence, DNA concentration, bacterial growth. These parameters were related to sensoribility of the pUC57-S-3M.

Parts used


/ / / / / / / /

Favorite Parts/ A promoter Biosenser


Project Planning Diary

Calender

May

-Brainstorming and recruiting.

June No entry

July

'August ' -We have been able to isolate a few colonies of transformants of E.coli containing the plasmid 19H (pSB1A2 or BBa I13543). We have been bent on picking up sequences containig lux genes.

September

-So far we have tried to isolate several plasmids but results have not been good. So far we have successfully amplified and isolated 4 plasmids. Well no one will believe how much we got done in such a small amout of time. [Laina's Diary]

October Determined that we will base our sensor on the concentrations of three metals ions, Vanadium, Iron and Nickel,and including elemental sulfur. Our focus is on the DNA expression, determined by luminesence and flourescence.

References

1.Cuero, R, Oullet T. Metal ions modulate gene expression and accumulation of the mycotoxins aflatoxin and zearalenone.J Appl Microbiol. 2005;98(3):598-605. �

2.Lovley, DR (2003). "Cleaning up with genomics: applying molecular biology to bioremediation". NATURE REVIEWS. MICROBIOLOGY. 1 (1): 35–44. PMID 15040178. Holmes et al 1993b. Holmes D.S et al, 1993. Development of Biosensor to Measure Metal Ions

3. Bioavailability in mining and metal waste.pp659-666. In Biohydrometallurgical Technologies. Vol. II. A.E Torma, M.L Apel and C.L Brierle,TMS, Warrendale, PA,USA.Eds.

4.Holmes, D.S. Dubey, U.K and Gongolli, 1993. Development of Biosensor for the detection of Mercury andCopper Ions.Environ, Geochem. Health 16: 229-233.

5.Leonard G. Davis, Mark D. Dibner, James F Battey. Basic Methods in Molecular Biology. Elsevier Science Publishers.1986, New York, Amsterdam and London.

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